Team:MIT/Projects

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Project 1: Metabolic Engineering of PCB Synthesis in Yeast

Brief Description

Phycocyanobilin (PCB) is a chromophore necessary for PhyB-PIF3 based synthetic devices. Our goal is to engineer a yeast strain capable of synthesizing PCB, so that no exogenous PCB would be needed for PhyB-PIF3 to function in yeast. We thus cloned the genes encoding enzymes in the PCB biosynthetic pathway for expression in yeast
Details about Project 1

Project 2: Rapid & Reversible Protein Localization using PhyB-PIF3 System

Brief Description

Our goal is to engineer a system that adopts the PhyB-PIF3 switch to control protein localization within the cell. In our design, either PhyB or PIF3 is constitutively anchored to one of the four target locations. The other will then be bound to our protein of interest and diffuse within the cell. Here, we use CFP and YFP to track the location of PhyB and PIF3, respectively. Reversable, light-dependent association of PhyB and PIF3 can then be monitored by fluorescence microscopy.
Details about Project 2

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 __NOTOC__
+[[Image:Bilibuddies_project.png]]
+<center>[[Team:MIT|Home]] | [[Team:MIT/Projects|Projects]] | [[Team:MIT/Protocols|Protocols]] | [http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2009&group=MIT Parts for Registry] | [[Team:MIT/References|References]]</center>
+<hr>
-<center>[[Team:MIT|Home]]|[[Team:MIT/Projects|Projects]]|[[Team:MIT/Protocols|Protocols]]|[[Team:MIT/PartsReg|Parts for Registry]]</center>
 ===Project 1: Metabolic Engineering of PCB Synthesis in Yeast===
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 {| cellspacing="5"
-|width="60%" class="green3" |
+|width="65%" |
-<!-- Project Description HEADER with edit link-->
+[[Image:PCB_factory.jpg|center]]
-<h3>IMAGE</h3>
-<hr/>
-<!-- END RESOURCES HEADER-->
-|width="40%" class="green3" |
-<!-- Project Description HEADER with edit link-->
+|width="35%" |
 <h3>Brief Description</h3>
 <hr/>
-<!-- END RESOURCES HEADER-->
-DESCRIPTION ........................................<br>
+Phycocyanobilin (PCB) is a chromophore necessary for PhyB-PIF3 based
-[[Team:MIT/Projects/Project1|Link to more details about Project 1]]
+synthetic devices. Our goal is to engineer a yeast strain capable of
+synthesizing PCB, so that no exogenous PCB would be needed for
+PhyB-PIF3 to function in yeast. We thus cloned the genes encoding
+enzymes in the PCB biosynthetic pathway for expression in yeast
+<br>
+[[Team:MIT/Projects/Project1|Details about Project 1]]
 |}
 ===Project 2: Rapid & Reversible Protein Localization using PhyB-PIF3 System===
 <hr class=divider>
 {| cellspacing="5"
-|width="60%" class="green3" |
+|width="65%" |
-<!-- Project Description HEADER with edit link-->
+[[Image:localization_system.jpg|center]]
-<h3>IMAGE</h3>
-<hr/>
-<!-- END RESOURCES HEADER-->
-|width="40%" class="green3" |
+|width="65%" |
-<!-- Project Description HEADER with edit link-->
 <h3>Brief Description</h3>
 <hr/>
-<!-- END RESOURCES HEADER-->
-DESCRIPTION ........................................<br>
+Our goal is to engineer a system that adopts the PhyB-PIF3 switch to
-[[Team:MIT/Projects/Project2|Link to more details about Project 2]]
+control protein localization within the cell. In our design, either
+PhyB or PIF3 is constitutively anchored to one of the four target
+locations. The other will then be bound to our protein of interest and
+diffuse within the cell. Here, we use CFP and YFP to track the
+location of PhyB and PIF3, respectively. Reversable, light-dependent
+association of PhyB and PIF3 can then be monitored by fluorescence
+microscopy.<br>
+[[Team:MIT/Projects/Project2|Details about Project 2]]
+|}