From 2009.igem.org
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| - | '''Ex 7:results:''' Ran .7% Gel for verification of DNA, good | + | '''Ex 7 results:''' Ran .7% Gel for verification of DNA, good |
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| | [[Image:gel 0605.jpg]] | | [[Image:gel 0605.jpg]] |
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| | 3. Brian’s RFS1010 derived Plasmid - rMMBRraA | | 3. Brian’s RFS1010 derived Plasmid - rMMBRraA |
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| | + | - transformed competent DH10B cells with pRL1383a and pSB1A3 (successful) - NP |
Latest revision as of 06:32, 20 October 2009
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| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/1_April_2009&action=edit 1]
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| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/3_April_2009&action=edit 3]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/4_April_2009&action=edit 4]
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| [http://2009.igem.org/Wisconsin-Madison/7_April_2009 7]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/8_April_2009&action=edit 8]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/9_April_2009&action=edit 9]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/10_April_2009&action=edit 10]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/11_April_2009&action=edit 11]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/12_April_2009&action=edit 12]
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| [http://2009.igem.org/Wisconsin-Madison/14_April_2009 14]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/15_April_2009&action=edit 15]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/16_April_2009&action=edit 16]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/17_April_2009&action=edit 17]
| [http://2009.igem.org/Wisconsin-Madison/18_April_2009 18]
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| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/21_April_2009&action=edit 21]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/22_April_2009&action=edit 22]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/23_April_2009&action=edit 23]
| [http://2009.igem.org/Wisconsin-Madison/24_April_2009 24]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/25_April_2009&action=edit 25]
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| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/1_May_2009&action=edit 1]
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| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/3_May_2009&action=edit 3]
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| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/4_May_2009&action=edit 4]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/5_May_2009&action=edit 5]
| [http://2009.igem.org/Wisconsin-Madison/6_May_2009 6]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/7_May_2009&action=edit 7]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/8_May_2009&action=edit 8]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/9_May_2009&action=edit 9]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/10_May_2009&action=edit 10]
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| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/11_May_2009&action=edit 11]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/12_May_2009&action=edit 12]
| [http://2009.igem.org/Wisconsin-Madison/13_May_2009 13]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/14_May_2009&action=edit 14]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/15_May_2009&action=edit 15]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/16_May_2009&action=edit 16]
| [http://2009.igem.org/Wisconsin-Madison/17_May_2009 17]
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| [http://2009.igem.org/Wisconsin-Madison/18_May_2009 18]
| [http://2009.igem.org/Wisconsin-Madison/19_May_2009 19]
| [http://2009.igem.org/Wisconsin-Madison/20_May_2009 20]
| [http://2009.igem.org/Wisconsin-Madison/21_May_2009 21]
| [http://2009.igem.org/Wisconsin-Madison/22_May_2009 22]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/23_May_2009&action=edit 23]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/24_May_2009&action=edit 24]
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| [http://2009.igem.org/Wisconsin-Madison/25_May_2009 25]
| [http://2009.igem.org/Wisconsin-Madison/26_May_2009 26]
| [http://2009.igem.org/Wisconsin-Madison/27_May_2009 27]
| [http://2009.igem.org/Wisconsin-Madison/28_May_2009 28]
| [http://2009.igem.org/Wisconsin-Madison/29_May_2009 29]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/30_May_2009&action=edit 30]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/31_May_2009&action=edit 31]
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| June
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| [http://2009.igem.org/Wisconsin-Madison/1_June_2009 1]
| [http://2009.igem.org/Wisconsin-Madison/2_June_2009 2]
| [http://2009.igem.org/Wisconsin-Madison/3_June_2009 3]
| [http://2009.igem.org/Wisconsin-Madison/4_June_2009 4]
| [http://2009.igem.org/Wisconsin-Madison/5_June_2009 5]
| [http://2009.igem.org/Wisconsin-Madison/6_June_2009 6]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/7_June_2009&action=edit 7]
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| [http://2009.igem.org/Wisconsin-Madison/8_June_2009 8]
| [http://2009.igem.org/Wisconsin-Madison/9_June_2009 9]
| [http://2009.igem.org/Wisconsin-Madison/10_June_2009 10]
| [http://2009.igem.org/Wisconsin-Madison/11_June_2009 11]
| [http://2009.igem.org/Wisconsin-Madison/12_June_2009 12]
| [http://2009.igem.org/Wisconsin-Madison/13_June_2009 13]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/14_June_2009&action=edit 14]
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| [http://2009.igem.org/Wisconsin-Madison/15_June_2009 15]
| [http://2009.igem.org/Wisconsin-Madison/16_June_2009 16]
| [http://2009.igem.org/Wisconsin-Madison/17_June_2009 17]
| [http://2009.igem.org/Wisconsin-Madison/18_June_2009 18]
| [http://2009.igem.org/Wisconsin-Madison/19_June_2009 19]
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| [http://2009.igem.org/Wisconsin-Madison/24_June_2009 24]
| [http://2009.igem.org/Wisconsin-Madison/25_June_2009 25]
| [http://2009.igem.org/Wisconsin-Madison/26_June_2009 26]
| [http://2009.igem.org/Wisconsin-Madison/27_June_2009 27]
| [http://2009.igem.org/Wisconsin-Madison/28_June_2009 28]
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| July
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| [http://2009.igem.org/Wisconsin-Madison/1_July_2009 1]
| [http://2009.igem.org/Wisconsin-Madison/2_July_2009 2]
| [http://2009.igem.org/Wisconsin-Madison/3_July_2009 3]
| [http://2009.igem.org/Wisconsin-Madison/4_July_2009 4]
| [http://2009.igem.org/Wisconsin-Madison/5_July_2009 5]
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| [http://2009.igem.org/Wisconsin-Madison/6_July_2009 6]
| [http://2009.igem.org/Wisconsin-Madison/7_July_2009 7]
| [http://2009.igem.org/Wisconsin-Madison/8_July_2009 8]
| [http://2009.igem.org/Wisconsin-Madison/9_July_2009 9]
| [http://2009.igem.org/Wisconsin-Madison/10_July_2009 10]
| [http://2009.igem.org/Wisconsin-Madison/11_July_2009 11]
| [http://2009.igem.org/Wisconsin-Madison/12_July_2009 12]
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| [http://2009.igem.org/Wisconsin-Madison/14_July_2009 14]
| [http://2009.igem.org/Wisconsin-Madison/15_July_2009 15]
| [http://2009.igem.org/Wisconsin-Madison/16_July_2009 16]
| [http://2009.igem.org/Wisconsin-Madison/17_July_2009 17]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/18_July_2009&action=edit 18]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/19_July_2009&action=edit 19]
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| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/21_July_2009&action=edit 21]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/22_July_2009&action=edit 22]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/23_July_2009&action=edit 23]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/24_July_2009&action=edit 24]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/25_July_2009&action=edit 25]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/26_July_2009&action=edit 26]
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| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/27_July_2009&action=edit 27]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/28_July_2009&action=edit 28]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/29_July_2009&action=edit 29]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/30_July_2009&action=edit 30]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/31_July_2009&action=edit 31]
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| [http://2009.igem.org/Wisconsin-Madison/1_August_2009 1]
| [http://2009.igem.org/Wisconsin-Madison/2_August_2009 2]
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| [http://2009.igem.org/Wisconsin-Madison/3_August_2009 3]
| [http://2009.igem.org/Wisconsin-Madison/4_August_2009 4]
| [http://2009.igem.org/Wisconsin-Madison/5_August_2009 5]
| [http://2009.igem.org/Wisconsin-Madison/6_August_2009 6]
| [http://2009.igem.org/Wisconsin-Madison/7_August_2009 7]
| [http://2009.igem.org/Wisconsin-Madison/8_August_2009 8]
| [http://2009.igem.org/Wisconsin-Madison/9_August_2009 9]
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| [http://2009.igem.org/Wisconsin-Madison/10_August_2009 10]
| [http://2009.igem.org/Wisconsin-Madison/11_August_2009 11]
| [http://2009.igem.org/Wisconsin-Madison/12_August_2009 12]
| [http://2009.igem.org/Wisconsin-Madison/13_August_2009 13]
| [http://2009.igem.org/Wisconsin-Madison/14_August_2009 14]
| [http://2009.igem.org/Wisconsin-Madison/15_August_2009 15]
| [http://2009.igem.org/Wisconsin-Madison/16_August_2009 16]
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| [http://2009.igem.org/Wisconsin-Madison/17_August_2009 17]
| [http://2009.igem.org/Wisconsin-Madison/18_August_2009 18]
| [http://2009.igem.org/Wisconsin-Madison/19_August_2009 19]
| [http://2009.igem.org/Wisconsin-Madison/20_August_2009 20]
| [http://2009.igem.org/Wisconsin-Madison/21_August_2009 21]
| [http://2009.igem.org/Wisconsin-Madison/22_August_2009 22]
| [http://2009.igem.org/Wisconsin-Madison/23_August_2009 23]
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| [http://2009.igem.org/Wisconsin-Madison/24_August_2009 24]
| [http://2009.igem.org/Wisconsin-Madison/25_August_2009 25]
| [http://2009.igem.org/Wisconsin-Madison/26_August_2009 26]
| [http://2009.igem.org/Wisconsin-Madison/27_August_2009 27]
| [http://2009.igem.org/Wisconsin-Madison/28_August_2009 28]
| [http://2009.igem.org/Wisconsin-Madison/29_August_2009 29]
| [http://2009.igem.org/Wisconsin-Madison/30_August_2009 30]
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| [http://2009.igem.org/Wisconsin-Madison/1_September_2009 1]
| [http://2009.igem.org/Wisconsin-Madison/2_September_2009 2]
| [http://2009.igem.org/Wisconsin-Madison/3_September_2009 3]
| [http://2009.igem.org/Wisconsin-Madison/4_September_2009 4]
| [http://2009.igem.org/Wisconsin-Madison/5_September_2009 5]
| [http://2009.igem.org/Wisconsin-Madison/6_September_2009 6]
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| [http://2009.igem.org/Wisconsin-Madison/7_September_2009 7]
| [http://2009.igem.org/Wisconsin-Madison/8_September_2009 8]
| [http://2009.igem.org/Wisconsin-Madison/9_September_2009 9]
| [http://2009.igem.org/Wisconsin-Madison/10_September_2009 10]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/11_September_2009&action=edit 11]
| [http://2009.igem.org/Wisconsin-Madison/12_September_2009 12]
| [http://2009.igem.org/Wisconsin-Madison/13_September_2009 13]
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| [http://2009.igem.org/Wisconsin-Madison/14_September_2009 14]
| [http://2009.igem.org/Wisconsin-Madison/15_September_2009 15]
| [http://2009.igem.org/Wisconsin-Madison/16_September_2009 16]
| [http://2009.igem.org/Wisconsin-Madison/17_September_2009 17]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/18_September_2009&action=edit 18]
| [http://2009.igem.org/Wisconsin-Madison/19_September_2009 19]
| [http://2009.igem.org/Wisconsin-Madison/20_September_2009 20]
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| [http://2009.igem.org/Wisconsin-Madison/22_September_2009 22]
| [http://2009.igem.org/Wisconsin-Madison/23_September_2009 23]
| [http://2009.igem.org/Wisconsin-Madison/24_September_2009 24]
| [http://2009.igem.org/Wisconsin-Madison/25_September_2009 25]
| [http://2009.igem.org/Wisconsin-Madison/26_September_2009 26]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/27_September_2009&action=edit 27]
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| [http://2009.igem.org/Wisconsin-Madison/28_September_2009 28]
| [http://2009.igem.org/Wisconsin-Madison/29_September_2009 29]
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| [http://2009.igem.org/Wisconsin-Madison/1_October_2009 1]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/2_October_2009&action=edit 2]
| [http://2009.igem.org/Wisconsin-Madison/3_October_2009 3]
| [http://2009.igem.org/Wisconsin-Madison/4_October_2009 4]
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| [http://2009.igem.org/Wisconsin-Madison/5_October_2009 5]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/6_October_2009&action=edit 6]
| [http://2009.igem.org/Wisconsin-Madison/7_October_2009 7]
| [http://2009.igem.org/Wisconsin-Madison/8_October_2009 8]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/9_October_2009&action=edit 9]
| [http://2009.igem.org/Wisconsin-Madison/10_October_2009 10]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/11_October_2009&action=edit 11]
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| [http://2009.igem.org/Wisconsin-Madison/13_October_2009 13]
| [http://2009.igem.org/Wisconsin-Madison/14_October_2009 14]
| [http://2009.igem.org/Wisconsin-Madison/15_October_2009 15]
| [http://2009.igem.org/Wisconsin-Madison/16_October_2009 16]
| [http://2009.igem.org/Wisconsin-Madison/17_October_2009 17]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/18_October_2009&action=edit 18]
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| [http://2009.igem.org/Wisconsin-Madison/20_October_2009 20]
| [http://2009.igem.org/Wisconsin-Madison/21_October_2009 21]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/22_October_2009&action=edit 22]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/23_October_2009&action=edit 23]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/24_October_2009&action=edit 24]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/25_October_2009&action=edit 25]
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| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/27_October_2009&action=edit 27]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/28_October_2009&action=edit 28]
| [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/29_October_2009&action=edit 29]
| [http://2009.igem.org/Wisconsin-Madison/30_October_2009 30]
| [http://2009.igem.org/Wisconsin-Madison/31_October_2009 31]
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June 5, 2009
Ex 5: Results Triple Freezer Test is good
Ex 6: Sample 1: Results: Nothing from visible testing with match (video)
Isopentenol is flammable and we expect a small poof or flame from the sample
Sample 2: Placed 2.5 ml of sample to into fresh 100ml LB, 50uL Amp, 73.5uL Cm, 100uL Tet
OD 600
1. 0.078
2. 0.067
3. 0.077
4. 0.112
5. 0.146
6. 0.208 Induced with 1.02g Arabinose
7. 0.406 Induced with 100uL IPTG
LET SIT OVER WEEKEND
Ex 7 results: Ran .7% Gel for verification of DNA, good
Primer: Spinach cDNA Primer Design
Other: research
Ex 8: Obtaining Additional Promotors: pSB1A3-1, BBa_J33210, rMMBRraA
1. Plated High copy BioBrick assembly plasmid
http://partsregistry.org/Part:pSB1A3
pSB1A3-1 is a high copy number plasmid carrying ampicillin resistance.
Well K1 of 2009 Spring Kit Plate 1 --- Plasmid pSB1A3 with part BBa_J04450
2. Broad host range BioBrick plasmid
http://partsregistry.org/wiki/index.php/Part:BBa_K125000
BBa_J33210 - low to medium copy number, derived from pRL1383a, Streptomycin
Spring 2009 Distribution, 2009 Kit Plate 3, Well 17B
3. Brian’s RFS1010 derived Plasmid - rMMBRraA
- transformed competent DH10B cells with pRL1383a and pSB1A3 (successful) - NP
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"
