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Virginia Commonwealth/23 June 2009
From 2009.igem.org
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==Tuesday 23 June 2009== | ==Tuesday 23 June 2009== | ||
===Results=== | ===Results=== | ||
| - | * | + | * n/a |
| - | + | ||
---- | ---- | ||
| + | |||
===Tasks=== | ===Tasks=== | ||
| + | * Perform Digestion | ||
| + | * Run a Gel Electrophoresis on Digests | ||
| + | * Perform Ligation | ||
---- | ---- | ||
| - | + | ||
| - | + | ||
====Wetlab==== | ====Wetlab==== | ||
*Digested these parts: BBa_J23102, pSB3K3 (1), E0240 (1) | *Digested these parts: BBa_J23102, pSB3K3 (1), E0240 (1) | ||
| - | *Gel | + | * Gel Electrophoresis |
| - | + | ** Steps for making the gel: | |
| - | **The digestion on the backbone did not work. The DNA had degraded, indicated by a sharp decrease in the concentration of the DNA sample that it was taken from. The cell stock of the biobrick is being grown overnight so that more plasmid DNA can be purified. | + | *** 0.50 g Agarose |
| + | *** 50 mL 1x TAE Buffer | ||
| + | *** 0.5 uL cyber safe stain. | ||
| + | *** Heat in microwave. Pour in plate. Rake out bubbles and place well mold. | ||
| + | |||
| + | ''Gel Results'' | ||
| + | [[Image:2009-06-23 digest01.jpg|left|caption|400px]] | ||
| + | {| cellpadding="10" border="1" padding="5" | ||
| + | |+ | ||
| + | |- | ||
| + | !Gel (from left to right)!! | ||
| + | |- | ||
| + | | kB Ladder || | ||
| + | |- | ||
| + | | J23102 || | ||
| + | |- | ||
| + | | pSB3K3 || | ||
| + | |- | ||
| + | | E0240 || | ||
| + | |- | ||
| + | | 100bp Ladder || | ||
| + | |} | ||
| + | * The ladders: 2uL were used with 4 uL of water | ||
| + | * The DNA samples: 4 uL of dye were used with 20uL of sample | ||
| + | |||
| + | *The digestion on the backbone did not work. The DNA had degraded, indicated by a sharp decrease in the concentration of the DNA sample that it was taken from. The cell stock of the biobrick is being grown overnight so that more plasmid DNA can be purified. | ||
*Ligation | *Ligation | ||
**Since the backbone piece was not present on the gel electrophoresis, the ligation was frozen and not transformed. Another ligation will be done tomorrow morning as well as purification of the backbone. | **Since the backbone piece was not present on the gel electrophoresis, the ligation was frozen and not transformed. Another ligation will be done tomorrow morning as well as purification of the backbone. | ||
Latest revision as of 18:47, 5 August 2009
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Contents |
Tuesday 23 June 2009
Results
- n/a
Tasks
- Perform Digestion
- Run a Gel Electrophoresis on Digests
- Perform Ligation
Wetlab
- Digested these parts: BBa_J23102, pSB3K3 (1), E0240 (1)
- Gel Electrophoresis
- Steps for making the gel:
- 0.50 g Agarose
- 50 mL 1x TAE Buffer
- 0.5 uL cyber safe stain.
- Heat in microwave. Pour in plate. Rake out bubbles and place well mold.
- Steps for making the gel:
Gel Results
| Gel (from left to right) | |
|---|---|
| kB Ladder | |
| J23102 | |
| pSB3K3 | |
| E0240 | |
| 100bp Ladder |
- The ladders: 2uL were used with 4 uL of water
- The DNA samples: 4 uL of dye were used with 20uL of sample
- The digestion on the backbone did not work. The DNA had degraded, indicated by a sharp decrease in the concentration of the DNA sample that it was taken from. The cell stock of the biobrick is being grown overnight so that more plasmid DNA can be purified.
- Ligation
- Since the backbone piece was not present on the gel electrophoresis, the ligation was frozen and not transformed. Another ligation will be done tomorrow morning as well as purification of the backbone.
