Team:Heidelberg/Parts

From 2009.igem.org

(Difference between revisions)
(→Parts)
(→Parts)
 
(32 intermediate revisions not shown)
Line 2: Line 2:
<html><body id="parts"></body></html>
<html><body id="parts"></body></html>
{|
{|
-
|-valign="top" border="0"
+
|-valign="top" border="0" style="margin-left: 2px;"
-
|width="650px" style="padding: 0 20px 0 0; background-color:#ede8e2"|
+
|width="650px" style="padding: 0 15px 15px 20px; background-color:#ede8e2"|
__NOTOC__
__NOTOC__
-
 
= Parts =
= Parts =
-
 
== Measurement Kit ==
== Measurement Kit ==
 +
[[Team:Heidelberg/Project_Measurement|''Main Article: Measurement'']]
{| class="wikitable centered"  style="margin: 1em auto 1em auto"
{| class="wikitable centered"  style="margin: 1em auto 1em auto"
Line 20: Line 19:
|}
|}
-
|width="250px" style="background-color:#d8d5d0"|
+
== Core promoters ==
 +
 
 +
[[Team:Heidelberg/Project_Measurement#Different_core_promoters_result_in_different_expression_strength|''Main Article: Core promoters'']]
 +
 
 +
The following Parts contain the JeT proximal promoter, and a core promoter as described. Core and proximal promoter are separated by a HindIII site. Parts can be applied for testing synthetic proximal promoters. The strength of promoters derived from these parts can be modified by [[Project_Measurement#Different_core_promoters_result_in_different_expression_strength|core promoter swapping]].
 +
 
 +
{| class="wikitable centered"  style="margin: 1em auto 1em auto"
 +
|- style="background-color:#99cccc;"
 +
! height=20px, width=250px | Name || width=150px | Part type || width=150 | Registry link
 +
|- style="background-color:#9BDDFF;"
 +
| style="font-weight:bold;" | JeT  || Regulatory || [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203112 Part:BBa_K203112]
 +
|- style="background-color:#9BDDFF;"
 +
| style="font-weight:bold;" | JeT proximal-CMV core || Regulatory || [http://partsregistry.org/Part:BBa_K203113 Part:BBa_K203113]
 +
|-
 +
|}
 +
 
 +
== Promoters created by RA-PCR ==
 +
 
 +
[[Team:Heidelberg/Project_Synthetic_promoters|''Main article: Synthetic promoters'']]
 +
 
 +
All contain the core promoter of [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203112 Part:BBa_K203112] and were screened / measured in [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203100 Part:BBa_K203100].
 +
 
 +
{| class="wikitable centered"  style="margin: 1em auto 1em auto"
 +
|- style="background-color:#99cccc;"
 +
! height=20px, width=250px | Name || width=150px | Part type || width=150 | Registry link
 +
|- style="background-color:#9BDDFF;"
 +
| style="font-weight:bold;" | Constitutive promoter; 0.4 REU  || Regulatory || [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203110 Part:BBa_K203110]
 +
|- style="background-color:#9BDDFF;"
 +
| style="font-weight:bold;" | Constitutive promoter; 1 REU || Regulatory || [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203118 Part:BBa_K203118]
 +
|- style="background-color:#9BDDFF;"
 +
| style="font-weight:bold;" | Constitutive promoter; 2 REU || Regulatory || [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203111 Part:BBa_K203111]
 +
|- style="background-color:#9BDDFF;"
 +
| style="font-weight:bold;" | Constitutive promoter; 2.5 REU || Regulatory || [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203109 Part:BBa_K203109]
 +
|- style="background-color:#9BDDFF;"
 +
| style="font-weight:bold;" | NF-&kappa;B regulated promoter || Regulatory || [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203119 Part:BBa_K203119]
 +
|- style="background-color:#9BDDFF;"
 +
| style="font-weight:bold;" | pPAR&gamma; regulated promoter 1 || Regulatory || [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203114 Part:BBa_K203114]
 +
|- style="background-color:#9BDDFF;"
 +
| style="font-weight:bold;" | pPAR&gamma; regulated promoter 2 || Regulatory || [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203115 Part:BBa_K203115]
 +
|-
 +
|}
 +
 
 +
== HEARTBEAT Predicted promoters ==
 +
[[Team:Heidelberg/HEARTBEAT_database| ''Main article: HEARTBEAT Database'']]
 +
{| class="wikitable centered"  style="margin: 1em auto 1em auto"
 +
|- style="background-color:#99cccc;"
 +
! height=20px, width=250px | Name || width=150px | Part type || width=150 | Registry link
 +
|- style="background-color:#9BDDFF;"
 +
| style="font-weight:bold;" |SREBP regulated promoter, predicted, tested HB8  || Regulatory || [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203125  Part:BBa_K203125 ]
 +
|- style="background-color:#9BDDFF;"
 +
| style="font-weight:bold;" |SREBP regulated promoter, predicted, tested HB9 || Regulatory || [http://partsregistry.org/Part:BBa_K203126 Part:BBa_K203126]
 +
|-
 +
|}
 +
 
 +
== Natural promoters ==
 +
 
 +
[[Team:Heidelberg/Project_Natural_promoters|''Main article: Natural promoters'']]
 +
 
 +
{| class="wikitable centered"  style="margin: 1em auto 1em auto"
 +
|- style="background-color:#99cccc;"
 +
! height=20px, width=250px | Name || width=150px | Part type || width=150 | Registry link
 +
|- style="background-color:#9BDDFF;"
 +
| style="font-weight:bold;" | LDL receptor promoter (sterol regulated)  || Regulatory || [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203105 Part:BBa_K203105]
 +
|- style="background-color:#9BDDFF;"
 +
| style="font-weight:bold;" | HMG-CoA synthase promoter (sterol regulated) || Regulatory || [http://partsregistry.org/Part:BBa_K203106 Part:BBa_K203106]
 +
|- style="background-color:#9BDDFF;"
 +
| style="font-weight:bold;" | c-Jun promoter (growth factor regulated) || Regulatory || [http://partsregistry.org/Part:BBa_K203107 Part:BBa_K203107]
 +
|-
 +
|}
 +
 
 +
== Targeting Signals ==
 +
 
 +
[[Team:Heidelberg/Project_SaO|''Main article: Outlook and Summary'']]
 +
 
 +
Protein domains for targeting to sub-cellular compartments.
 +
 
 +
{| class="wikitable centered"  style="margin: 1em auto 1em auto"
 +
|- style="background-color:#99cccc;"
 +
! height=20px, width=250px | Name || width=150px | Part type || width=150 | Registry link
 +
|- style="background-color:#9BDDFF;"
 +
| style="font-weight:bold;" | Plasma membrane targeting (GPI anchor)  || Protein domain || [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203000 Part:BBa_K203000]
 +
|- style="background-color:#9BDDFF;"
 +
| style="font-weight:bold;" | Plasma membrane targeting (myrpalm) || Protein domain || [http://partsregistry.org/Part:BBa_K203108 Part:BBa_K203108]
 +
|- style="background-color:#9BDDFF;"
 +
| style="font-weight:bold;" | ER membrane targeting (C-terminal) || Protein domain || [http://partsregistry.org/Part:BBa_K203101 Part:BBa_K203101]
 +
|- style="background-color:#9BDDFF;"
 +
| style="font-weight:bold;" | ER membrane targeting (N-terminal) || Protein domain || [http://partsregistry.org/Part:BBa_K203124 Part:BBa_K203124]
 +
|- style="background-color:#9BDDFF;"
 +
| style="font-weight:bold;" | mCherry-myrpalm-polyA || Composite || [http://partsregistry.org/Part:BBa_K203116 Part:BBa_K203116]
 +
|-
 +
|- style="background-color:#9BDDFF;"
 +
| style="font-weight:bold;" |  GFP-polyA || Composite || [http://partsregistry.org/Part:BBa_K203117 Part:BBa_K203117]
 +
|-
 +
|}
 +
 
 +
|width="250px" style="padding: 0 20px 15px 15px; background-color:#d8d5d0"|
|}
|}

Latest revision as of 09:06, 4 July 2011

Parts

Measurement Kit

Main Article: Measurement

Name Part type Registry link
pSMB_MEASURE: Promoter Measurement plasmid (mammalian) Plasmid backbone [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203100 Part:BBa_K203100]
pSMB_REFERENCE Plasmid [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203099 Part:BBa_K203099]

Core promoters

Main Article: Core promoters

The following Parts contain the JeT proximal promoter, and a core promoter as described. Core and proximal promoter are separated by a HindIII site. Parts can be applied for testing synthetic proximal promoters. The strength of promoters derived from these parts can be modified by core promoter swapping.

Name Part type Registry link
JeT Regulatory [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203112 Part:BBa_K203112]
JeT proximal-CMV core Regulatory [http://partsregistry.org/Part:BBa_K203113 Part:BBa_K203113]

Promoters created by RA-PCR

Main article: Synthetic promoters

All contain the core promoter of [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203112 Part:BBa_K203112] and were screened / measured in [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203100 Part:BBa_K203100].

Name Part type Registry link
Constitutive promoter; 0.4 REU Regulatory [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203110 Part:BBa_K203110]
Constitutive promoter; 1 REU Regulatory [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203118 Part:BBa_K203118]
Constitutive promoter; 2 REU Regulatory [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203111 Part:BBa_K203111]
Constitutive promoter; 2.5 REU Regulatory [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203109 Part:BBa_K203109]
NF-κB regulated promoter Regulatory [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203119 Part:BBa_K203119]
pPARγ regulated promoter 1 Regulatory [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203114 Part:BBa_K203114]
pPARγ regulated promoter 2 Regulatory [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203115 Part:BBa_K203115]

HEARTBEAT Predicted promoters

Main article: HEARTBEAT Database

Name Part type Registry link
SREBP regulated promoter, predicted, tested HB8 Regulatory [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203125 Part:BBa_K203125 ]
SREBP regulated promoter, predicted, tested HB9 Regulatory [http://partsregistry.org/Part:BBa_K203126 Part:BBa_K203126]

Natural promoters

Main article: Natural promoters

Name Part type Registry link
LDL receptor promoter (sterol regulated) Regulatory [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203105 Part:BBa_K203105]
HMG-CoA synthase promoter (sterol regulated) Regulatory [http://partsregistry.org/Part:BBa_K203106 Part:BBa_K203106]
c-Jun promoter (growth factor regulated) Regulatory [http://partsregistry.org/Part:BBa_K203107 Part:BBa_K203107]

Targeting Signals

Main article: Outlook and Summary

Protein domains for targeting to sub-cellular compartments.

Name Part type Registry link
Plasma membrane targeting (GPI anchor) Protein domain [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203000 Part:BBa_K203000]
Plasma membrane targeting (myrpalm) Protein domain [http://partsregistry.org/Part:BBa_K203108 Part:BBa_K203108]
ER membrane targeting (C-terminal) Protein domain [http://partsregistry.org/Part:BBa_K203101 Part:BBa_K203101]
ER membrane targeting (N-terminal) Protein domain [http://partsregistry.org/Part:BBa_K203124 Part:BBa_K203124]
mCherry-myrpalm-polyA Composite [http://partsregistry.org/Part:BBa_K203116 Part:BBa_K203116]
GFP-polyA Composite [http://partsregistry.org/Part:BBa_K203117 Part:BBa_K203117]
Retrieved from "http://2009.igem.org/Team:Heidelberg/Parts"