"
Team:Uppsala-Sweden/Butanol
From 2009.igem.org
Karl.brune (Talk | contribs) (→References) |
(→Progress) |
||
| (9 intermediate revisions not shown) | |||
| Line 2: | Line 2: | ||
==The Butanol Approach== | ==The Butanol Approach== | ||
| - | Our goal is to achieve photosynthetic production of butanol and its | + | Our goal is to achieve photosynthetic production of butanol and its derivatives with the cyanobacterium <i>Synechocystis sp. PCC 6803</i> as the final host organism. |
==Background== | ==Background== | ||
| - | Cyanobacteria have the capability to harvest the energy from the sun and convert it into other forms of energy. The natural way for these organisms is to store it as sugars or other carbohydrates in a way similar to plants. By introducing a | + | Cyanobacteria have the capability to harvest the energy from the sun and convert it into other forms of energy. The natural way for these organisms is to store it as sugars or other carbohydrates in a way similar to plants. By introducing a cassette of genes for isobutanol production, we would like to derive a cyanobacteria that produces butanol and its derivatives. |
==The Constructs== | ==The Constructs== | ||
| - | We designed | + | We designed two final constructs (<partinfo>BBa_K273011</partinfo> & <partinfo>BBa_K273012</partinfo>) that actually only differ in the promoter, as our final host organism <i>Synechocystis sp PCC6803</i> does not allow the use of conventional inducible promoters. More information about pPetE, a copper inducible promoter for <i>Synechocystis sp PCC6803</i>, can be found here <partinfo>K273019</partinfo>. |
<h3><partinfo>BBa_K273011</partinfo> : The Construct for Testing Purposes in <i>E. Coli</i></h3> | <h3><partinfo>BBa_K273011</partinfo> : The Construct for Testing Purposes in <i>E. Coli</i></h3> | ||
| Line 20: | Line 20: | ||
<center>[[Image:Butanol construct.png]]</center> | <center>[[Image:Butanol construct.png]]</center> | ||
| - | |||
==The Pathway== | ==The Pathway== | ||
| - | The original pathway to obtain branched-chain higher alcohols was proposed and | + | The original pathway to obtain branched-chain higher alcohols was proposed and successfully established in <i>E. coli</i> by Shota Atsumi, Taizo Hanai and James C. Liao from the University of Californa, Los Angeles. [[#ref1|<nowiki>[1]</nowiki>]] As we expect a broad range of higher alcohols, we will exemplary demonstrate the biochemical mechanism with the pathway of isobutanol, the product we expect to be the most abundant. |
<center>[[Image:butanolpath_horizontal.png]]</center> | <center>[[Image:butanolpath_horizontal.png]]</center> | ||
| - | The precursor for isobutanol production is pyruvate, a central metabolite which is usually derived by the | + | The precursor for isobutanol production is pyruvate, a central metabolite which is usually derived by the glycolysis. Two pyruvate molecules are converted first to 2-acetolactate and then subsequently to 2,3-dihydroxy-isovalerate. A further dehydration reaction results in our first molecule of interest, 2-ketoisovalerate, which is a precursor for the synthesis of the amino acids valine, alanine and leucine. [[#ref2|<nowiki>[2]</nowiki>]] |
| - | So far, | + | So far a native pathway has been utilized, our first BioBrick <partinfo>K273006</partinfo> for the Butanol approach, the codon-optimized version of the alpha-ketoisovalerate decarboxylase (kivd) from <i>Lactococcus lactis ssp. lactis</i> is operating on these products. [[#ref3|<nowiki>[3]</nowiki>]] |
| - | This particular enzyme performs a decarboylation reaction, thus transforms 2-ketoisovalerate to an aldehyde, isobutanal. [[#ref4|<nowiki>[4]</nowiki>]] | + | This particular enzyme performs a decarboylation reaction, and thus transforms 2-ketoisovalerate to an aldehyde, isobutanal. [[#ref4|<nowiki>[4]</nowiki>]] |
Subsequently isobutanal will be oxidized to isobutanol by an enzyme which is encoded by our BioBrick <partinfo>K273005</partinfo>, the alcohol dehydrogenase 2 (adh2) from <i>S. cerevisiae</i>. [[#ref5|<nowiki>[5]</nowiki>]] | Subsequently isobutanal will be oxidized to isobutanol by an enzyme which is encoded by our BioBrick <partinfo>K273005</partinfo>, the alcohol dehydrogenase 2 (adh2) from <i>S. cerevisiae</i>. [[#ref5|<nowiki>[5]</nowiki>]] | ||
| + | |||
| + | ==Status== | ||
| + | ''as of 2009-10-21'' | ||
| + | |||
| + | The Butanol construct is completed and is currently being evaluated in E.coli. While the transformation to Synechocystis is done it takes approximately two weeks for the colonies to grow to large enough size to be used in testing. | ||
| + | |||
| + | [[Image:Progressbar_butanol.png]] | ||
==References== | ==References== | ||
| Line 52: | Line 58: | ||
<nowiki>[5]</nowiki> [http://biocyc.org/META/NEW-IMAGE?type=REACTION&object=RXN-7657 MetaCyc Reaction: 1.1.1.1] | <nowiki>[5]</nowiki> [http://biocyc.org/META/NEW-IMAGE?type=REACTION&object=RXN-7657 MetaCyc Reaction: 1.1.1.1] | ||
{{Uppsala-Sweden_Footer}} | {{Uppsala-Sweden_Footer}} | ||
| + | |||
| + | |||
| + | ==Safety== | ||
| + | Please find information here: | ||
| + | [https://2009.igem.org/Team:Uppsala-Sweden/Safety Safety during the Uppsala iGEM 2009 Project] | ||
Latest revision as of 03:17, 22 October 2009
Contents |
The Butanol Approach
Our goal is to achieve photosynthetic production of butanol and its derivatives with the cyanobacterium Synechocystis sp. PCC 6803 as the final host organism.
Background
Cyanobacteria have the capability to harvest the energy from the sun and convert it into other forms of energy. The natural way for these organisms is to store it as sugars or other carbohydrates in a way similar to plants. By introducing a cassette of genes for isobutanol production, we would like to derive a cyanobacteria that produces butanol and its derivatives.
The Constructs
We designed two final constructs ( & ) that actually only differ in the promoter, as our final host organism Synechocystis sp PCC6803 does not allow the use of conventional inducible promoters. More information about pPetE, a copper inducible promoter for Synechocystis sp PCC6803, can be found here .
: The Construct for Testing Purposes in E. Coli
: The Final Construct for Synechocystis sp PCC6803
The Pathway
The original pathway to obtain branched-chain higher alcohols was proposed and successfully established in E. coli by Shota Atsumi, Taizo Hanai and James C. Liao from the University of Californa, Los Angeles. [1] As we expect a broad range of higher alcohols, we will exemplary demonstrate the biochemical mechanism with the pathway of isobutanol, the product we expect to be the most abundant.
The precursor for isobutanol production is pyruvate, a central metabolite which is usually derived by the glycolysis. Two pyruvate molecules are converted first to 2-acetolactate and then subsequently to 2,3-dihydroxy-isovalerate. A further dehydration reaction results in our first molecule of interest, 2-ketoisovalerate, which is a precursor for the synthesis of the amino acids valine, alanine and leucine. [2]
So far a native pathway has been utilized, our first BioBrick for the Butanol approach, the codon-optimized version of the alpha-ketoisovalerate decarboxylase (kivd) from Lactococcus lactis ssp. lactis is operating on these products. [3]
This particular enzyme performs a decarboylation reaction, and thus transforms 2-ketoisovalerate to an aldehyde, isobutanal. [4]
Subsequently isobutanal will be oxidized to isobutanol by an enzyme which is encoded by our BioBrick , the alcohol dehydrogenase 2 (adh2) from S. cerevisiae. [5]
Status
as of 2009-10-21
The Butanol construct is completed and is currently being evaluated in E.coli. While the transformation to Synechocystis is done it takes approximately two weeks for the colonies to grow to large enough size to be used in testing.
References
[1] [http://www.ncbi.nlm.nih.gov/pubmed/18172501 Non-fermentative pathways for synthesis of branched-chain higher alcohols as biofuels. Atsumi S, Hanai T, Liao JC Nature. 2008 Jan 3;451(7174):86-9]
[2] [http://biocyc.org/META/NEW-IMAGE?type=PATHWAY&object=VALSYN-PWY MetaCyc Pathway: valine biosynthesis]
[3] [http://www.ncbi.nlm.nih.gov/pubmed/15358422 Biochemical and molecular characterization of alpha-ketoisovalerate decarboxylase, an enzyme involved in the formation of aldehydes from amino acids by Lactococcus lactis. de la Plaza M, Fernández de Palencia P, Peláez C, Requena T. FEMS Microbiol Lett. 2004 Sep 15;238(2):367-74]
[4] [http://biocyc.org/META/NEW-IMAGE?type=REACTION&object=RXN-7643 MetaCyc Reaction: 4.1.1.1]
[5] [http://biocyc.org/META/NEW-IMAGE?type=REACTION&object=RXN-7657 MetaCyc Reaction: 1.1.1.1]
Safety
Please find information here: Safety during the Uppsala iGEM 2009 Project
