Team:Waterloo/Notebook/Oligos
From 2009.igem.org
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! Description !! Oligo Name !! Sequence !! Length | ! Description !! Oligo Name !! Sequence !! Length | ||
+ | |||
|- | |- | ||
- | | | + | |amplifies first half of ''E. coli'' α-galactosidase gene adds SalI site (fwd) || HI-SalI-F || CATGTCGACATGATGTCTGCACCCAAAATT || 30 |
|- | |- | ||
- | | | + | |amplifies first half of α-galactosidase gene adds BamHI site (rev) || HI-BamHI-attP-R || ATGGGATCCCCCCCAACTGAGAGAACTCAAAGGTTACCCCAGTTGGGGGTAACGCAGCGTAGCTGG || 66 |
+ | |- | ||
+ | |amplifies second half of α-galactosidase gene, adds KpnI site (fwd) || H2-KpnI-attP-F || CATGGTACCCCCCCAACTGAGAGAACTCAAAGGTTACCCCAGTTGGGGGCGTTTTACCTGGAGCTGG || 67 | ||
+ | |- | ||
+ | |amplifies second half of α-galactosidase gene, adds XbaI site (rev) || H2-XbaI-R || ATGTCTAGAGGTCAGCAGCGTCTGT || 25 | ||
+ | |- | ||
+ | |Amplifes any biobrick, adds EcoRV end for ligation into EcoRV blunt ends (fwd) || Prefix-EcoRV-F || ATCGAATTCGCGGCCGCTTCTAG || 23 | ||
+ | |- | ||
+ | |Amplifes any biobrick, adds EcoRV end for ligation into EcoRV blunt ends (rev) || Suffix-EcoRV-R || ATCCTGCAGCGGCCGCTACTAGTA || 24 | ||
|- | |- | ||
|B0034 RBS primer, use with VF2 (fwd) || Prefix-RBS || GGCCGCTTCTAGAGAAAGAGG || 21 | |B0034 RBS primer, use with VF2 (fwd) || Prefix-RBS || GGCCGCTTCTAGAGAAAGAGG || 21 | ||
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|B0034 RBS primer, use with VR (rev)|| Suffix-RBS || TTTCTCCTCTTTCTCTAGATGCGG || 24 | |B0034 RBS primer, use with VR (rev)|| Suffix-RBS || TTTCTCCTCTTTCTCTAGATGCGG || 24 | ||
|- | |- | ||
- | | | + | |ordered to add Plac and RBS to the cI ORF (project 2008) || Plac-RBS-F || GAATTCGCGGCCGCTTCTAGAGAATTGTGAGCGGATAACAATTGACATTGTGAGCGGATAA CAAGATACTGAGCACATACTAGAGAAAGAGGAGAAATACTAG || 103 |
|- | |- | ||
- | | | + | |amplifies ΦC31 int, adds RBS, Prefix, changes start codon to ATG, changes G to A maintaining glutamic acid codon and eliminating EcoRI site (fwd) || Int-BBa-F || GAATTCGCGGCCGCTTCTAGAGAAAGAGGAGAAATACTAGATGGACACGTACGCGGGTGCTT ACGACCGTCAGTCGCGCGAGCGCGAAAATTCG || 94 |
|- | |- | ||
- | | | + | |amplifies ΦC31 int, adds suffix, changes stop codon to double TAA (rev) || Int-DSC-R || CTGCAGCGGCCGCTACTAGTATTATTACGCCGCTACGTCTTCCGTG || 46 |
|- | |- | ||
- | |Gene 6 | + | |amplifies T7 Gene 6 exonuclease, adds ATG start codon, adds BBa prefix (fwd2) || T7gene6-F2 || GAATTCGCGGCCGCTTCTAGATGGCACTTCTTGACCTTAAAC || 42 |
|- | |- | ||
- | | | + | |does not add standard start codon (fwd) || T7gene6-F || CAATGGAATTCGCGGCCGCTTCTAGATGGCACTTCTTGACCTTAAA || 46 |
|- | |- | ||
- | | | + | |amplifies T7 Gene 6 exonuclease, adds double TAA stop codon, adds BBa suffix (rev2) || T7G6-R2 || CTGCAGCGGCCGCTACTAGTATTATTACGGTCTCCACAGGTAAATCTC || 48 |
|- | |- | ||
- | | | + | |does not add standard stop codon (rev) || T7gene6-R || CAATTTCTGCAGCGGCCGCTACTAGTACTACGGTCTCCACAGG || 43 |
|- | |- | ||
- | | | + | |amplifies T7 G6 makes product for cross over PCR with ''pmeI'' cross over PCR product || T7g6-RXO-RBS || CTAGTATTTCTCCTCTTTCTCTAGTATTATTACGGTCTCCACAGGTAAATCTC || 53 |
|- | |- | ||
- | | | + | |Amplifies T7 gene 5 exonuclease, a 3′-5′ exonuclease, adds BBa prefix (fwd) || T7gene5-F || CAATGGAATTCGCGGCCGCTTCTAGATGATCGTTTCTGACATCGAA || 46 |
|- | |- | ||
- | | | + | |Amplifies T7 gene 5 exonuclease, a 3′-5′ exonuclease, adds BBa suffix (rev) || T7gene5-R || CAATTTCTGCAGCGGCCGCTACTAGTATCAGTGGCAAATCGCCCAATTAGG || 51 |
|- | |- | ||
- | | | + | |amplifies phbA (fwd) || phbA-F || GAATTCGCGGCCGCTTCTAGATGAGCAATCCCTCGATCG || 39 |
|- | |- | ||
- | | | + | |amplifies phbA, non standard stop codon (rev) || phbB-R || CTGCAGCGGCCGCTACTAGTATTACAGGCGTTCCACGCACATGG || 44 |
|- | |- | ||
- | | | + | |amplifies phbB (fwd)|| phbB-F || GAATTCGCGGCCGCTTCTAGATGAGCAGGGTAGCACTGG || 39 |
|- | |- | ||
- | | | + | |amplifies phbB, non standard stop codon (rev)|| phbB-R || CTGCAGCGGCCGCTACTAGTA TCAGGCGAAGTACTGGCCGC || 41 |
|- | |- | ||
- | | | + | |amplifies phbC (fwd) || phbC-F || GAATTCGCGGCCGCTTCTAGATGACAGCAGAGAAGGCTGAGG || 42 |
|- | |- | ||
- | | | + | |amplifies phbC, non standard stop codon (rev) || phbC-R || CTGCAGCGGCCGCTACTAGTATCAGGCGCGCACGCGCACGTAGG || 44 |
|- | |- | ||
- | | | + | |amplifes RFP with [http://partsregistry.org/Part:BBa_J23118 BBa_J23118] constitutive promoter ([http://partsregistry.org/Part:BBa_K093012 BBa_K093012]) anneals to promoter sequence (fwd) || RFP-BamHI-F || CATGGATCCTTGACGGCTAGCTCAGTC || 27 |
|- | |- | ||
- | | | + | |anneals to RFP ORF sequence adds TT ([http://partsregistry.org/Part:BBa_B1002 BBa_B1002]) adds KpnI site, but don't use it to clone, the ORF contains other KpnI sites || RFP-KpnII-TT-R (rev) || ATGGGTACCAAAAAAAAACCCCGCCGAAGCGGGGTTTTTTTTTCTCTAGTAGCGATCTACACTAGCACTATCA || 73 |
|- | |- | ||
- | | | + | |amplifies ''pmeI'' from ''Pseudomonas mendocina'' NEB698, used to amplify synthetic ORF to add RBS (fwd)|| PmeIR-F || GAATTCCGGCCGCTTCTAGAGAAAGAGGAGAAATACTAGATGACAACAAACTCCCCCTCAG || 61 |
|- | |- | ||
- | | | + | |amplifies ''pmeI'' from ''Pseudomonas mendocina'' NEB698, or our synthetic ORF (rev) || PmeIR-R || CTGCAGCGGCCGCTACTAGTATTATTAAACCGTACTTCGCCCCCG || 45 |
|- | |- | ||
- | | | + | |sense oligo for RecA-lexA hybrid promoter ([http://partsregistry.org/Part:BBa_K093000 BBa_K093000]) 5' EcoRI overhang (fwd) || recA-F || AATTCGCGGCCGCTTCTAGAGTACAAAACACTTGATACTGTATATATATACAGTATAATTGCTTCAACATA|| 71 |
|- | |- | ||
- | | | + | |sense oligo for RecA lexA hybrid promoter ([http://partsregistry.org/Part:BBa_K093000 BBa_K093000]) 3' SpeI overhang (rev) || recA-R || CTAGTATGTTGAAGCAATTATACTGTATATATATACAGTATCAAGTGTTTTGTACTCTAGAAGCGGCCGCG || 71 |
|- | |- | ||
- | |RFP ORF (fwd | + | |amplifies RFP ORF adds RBS (fwd) || RFP-RBS-F || GAATTCCGGCCGCTTCTAGAGAAAGAGGAGAAATACTAGATGGCTTCCTCCGAAGACG || 58 |
|- | |- | ||
- | |RFP ORF | + | |amplifies RFP ORF, creates product for crossover PCR || RFP-RXO || GATGCCTGGCTCTAGTATTATTAAGCACCGGTGG || 34 |
|- | |- | ||
- | | | + | |Amplifies RK2 oriT adds MunI site (compatible end with EcoRI) (fwd) || RK2E-F || CATCAATTGCCGGCCAGCCTCGCAGAGCA || 29 |
- | | | + | |- |
- | + | |Amplifies RK2 oriT adds EcoRI site, adds attB site in the forward orientation (rev) || RK2E-R || ATGGAATTCCGCGCCCGGGGAGCCCAAGGGCACGCCCTGGCACCCCGGGCAGGATAGGTGAAGT || 64 | |
+ | |- | ||
+ | |ampifies RK2 oriT adds PstI site, adds attB in the reverse orientation (fwd) || RK2-FP || CATCTGCAGCGCGCCCGGGGAGCCCAAGGGCACGCCCTGGCACCCCGGCCAGCCTCGCAGAGCA || 64 | ||
+ | |- | ||
+ | |amplifes RK2 oriT adds Mph1103I site (compatible end with PstI) (rev) || RK2-RP || ATGATGCATCCGGGCAGGATAGGTGAAGT || 29 | ||
+ | |- | ||
+ | |BBa sequencing/colony PCR primer (fwd) || VF2 || TGCCACCTGACGTCTAAGAA || 20 | ||
+ | |- | ||
+ | |BBa sequencing/colony PCR primer (rev) || VR || ATTACCGCCTTTGAGTGAGC || 20 | ||
+ | |- | ||
+ | |sequencing c31 int gene anneals internally | ||
+ | |Int seq F | ||
+ | |cgtgcaacgtcagcatggcg | ||
+ | |- | ||
+ | |sequenceing c31 int gene anneals internally | ||
+ | |Int seq2 R | ||
+ | |cgccatgctgacgttgcacg | ||
+ | |- | ||
+ | |sequenceing c31 int gene anneals internally | ||
+ | |Int seq3 R | ||
+ | |caccaccaccggattacgtc | ||
+ | |- | ||
+ | |was designed to fix an error in another PCR primer that was thrown out. Could still amplify biobricked c31 int | ||
+ | |Int RBS | ||
+ | |GAATTCGCGGCCGCTTCTAGAGAAAGAGGAGAAATACTAGATGGAC | ||
+ | |- | ||
+ | |sequencing primer for pKNG101 derived vectors (pFB9XXXX) | ||
+ | |pKNG101 F | ||
+ | |CTACATATCACAACGTGCGTG | ||
+ | |- | ||
+ | |sequencing primer for pKNG101 derived vectors (pFB9XXXX) | ||
+ | |pKNG101 R | ||
+ | |CTCCGTCTACTGTACGATACAC | ||
+ | |- | ||
+ | |linker+ for synthetic attP cloning into pKNG101 derived vectors | ||
+ | |FB attP BamHI F | ||
+ | |CTCAGTTGGGGGG | ||
+ | |- | ||
+ | |linker- for synthetic attP cloning into pKNG101 derived vectors | ||
+ | |FB attP BamHI R | ||
+ | |GATCCCCC | ||
+ | |- | ||
+ | |linker+ for synthetic attP cloning into pKNG101 derived vectors | ||
+ | |FB attP SalI F | ||
+ | |TCGACCCC | ||
+ | |- | ||
+ | |linker- for synthetic attP cloning into pKNG101 derived vectors | ||
+ | |FB attP SalI R | ||
+ | |CCCAGTTGGGGG | ||
+ | |- | ||
+ | |Bba linker+ attB | ||
+ | |BBa attB EcoRI F | ||
+ | |AATTCGCGGCCGCTTCTAGAG | ||
+ | |- | ||
+ | |Bba linker- attB | ||
+ | |BBa attB EcoRI R | ||
+ | |CACCCTCTAGAAGCGGCCGCG | ||
+ | |- | ||
+ | |Bba linker+ attB | ||
+ | |BBa attB PstI F | ||
+ | |CGCGTACTAGTAGCGGCCGCTGCA | ||
+ | |- | ||
+ | |Bba linker- attB | ||
+ | |BBa attB PstI R | ||
+ | |GCGGCCGCTACTAGTA | ||
+ | |- | ||
+ | |BBa linker+ attP | ||
+ | |BBa attP EcoRI fwd | ||
+ | |aattcgcggccgcttctagagCCC | ||
+ | |24 | ||
+ | |- | ||
+ | |BBa linker- attP | ||
+ | |BBa attP EcoRI rev | ||
+ | |CCCAGTTGGGGctctagaagcggccgcg | ||
+ | |- | ||
+ | |BBa linker+ attP | ||
+ | |BBa attP PstI fwd | ||
+ | |CTCAGTTGGGGGGtactagtagcggccgctgca | ||
+ | |33 | ||
+ | |- | ||
+ | |BBa linker- attP | ||
+ | |BBa attP PstI rev | ||
+ | |gcggccgctactagtaCCCC | ||
+ | |20 | ||
+ | |- | ||
+ | |attB +strand | ||
+ | |attB34 TG F | ||
+ | |GGTGCCAGGGCGTGCCCTGGGGCTCCCCGGG | ||
+ | |- | ||
+ | |attB -strand | ||
+ | |attB34 TG R | ||
+ | |CGCGCCCGGGGAGCCCCAGGGCACGCCCTGG | ||
+ | |- | ||
+ | |attB +strand | ||
+ | |attB34 CT F | ||
+ | |GGTGCCAGGGCGTGCCCCTGGGCTCCCCGGG | ||
+ | |- | ||
+ | |attB -strand | ||
+ | |attB34 CT R | ||
+ | |CGCGCCCGGGGAGCCCAGGGGCACGCCCTGG | ||
+ | |- | ||
+ | |attB +strand | ||
+ | |attB34 TT F | ||
+ | |GGTGCCAGGGCGTGCCCTTGGGCTCCCCGGG | ||
+ | |- | ||
+ | |attB -strand | ||
+ | |attB34 TT R | ||
+ | |CGCGCCCGGGGAGCCCAAGGGCACGCCCTGG | ||
+ | |- | ||
+ | |attB +strand | ||
+ | |attB 34 GA F | ||
+ | |GGTGCCAGGGCGTGCCCGAGGGCTCCCCGGG | ||
+ | |- | ||
+ | |attB -strand | ||
+ | |attB34 GA R | ||
+ | |CGCGCCCGGGGAGCCCTCGGGCACGCCCTGG | ||
+ | |- | ||
+ | |attB +strand | ||
+ | |attB34 AC F | ||
+ | |GGTGCCAGGGCGTGCCCACGGGCTCCCCGGG | ||
+ | |- | ||
+ | |attB -strand | ||
+ | |attB34 AC R | ||
+ | |CGCGCCCGGGGAGCCCGTGGGCACGCCCTGG | ||
+ | |- | ||
+ | |attB +strand | ||
+ | |attB34 GG F | ||
+ | |GGTGCCAGGGCGTGCCCGGGGGCTCCCCGGG | ||
+ | |- | ||
+ | |attB -strand | ||
+ | |attB34 GG R | ||
+ | |CGCGCCCGGGGAGCCCCCGGGCACGCCCTGG | ||
+ | |- | ||
+ | |attP + strand | ||
+ | |attP39 TG F | ||
+ | |CAACTGGGGTAACCTTGGAGTTCT | ||
+ | |- | ||
+ | |attP - strand | ||
+ | |attP39 TG R | ||
+ | |CCAACTGAGAGAACTCCAAGGTTAC | ||
+ | |- | ||
+ | |attP + strand | ||
+ | |attP39 CT F | ||
+ | |CAACTGGGGTAACCTCTGAGTTCT | ||
+ | |- | ||
+ | |attP - strand | ||
+ | |attP39 CT R | ||
+ | |CCAACTGAGAGAACTCAGAGGTTAC | ||
+ | |- | ||
+ | |attP + strand | ||
+ | |attP39 TT F | ||
+ | |CAACTGGGGTAACCTTTGAGTTCT | ||
+ | |- | ||
+ | |attP - strand | ||
+ | |attP39 TT R | ||
+ | |CCAACTGAGAGAACTCAAAGGTTAC | ||
+ | |- | ||
+ | |attP + strand | ||
+ | |attP39 GA F | ||
+ | |CAACTGGGGTAACCTGAGAGTTCT | ||
+ | |- | ||
+ | |attP - strand | ||
+ | |attP39 GA R | ||
+ | |CCAACTGAGAGAACTCTCAGGTTAC | ||
+ | |- | ||
+ | |attP + strand | ||
+ | |attP39 AC F | ||
+ | |CAACTGGGGTAACCTACGAGTTCT | ||
+ | |- | ||
+ | |attP - strand | ||
+ | |attP39 AC R | ||
+ | |CCAACTGAGAGAACTCGTAGGTTAC | ||
+ | |- | ||
+ | |attP + strand | ||
+ | |attP39 GG F | ||
+ | |CAACTGGGGTAACCTGGGAGTTCT | ||
+ | |- | ||
+ | |attP - strand | ||
+ | |attP39 GG R | ||
+ | |CCAACTGAGAGAACTCCCAGGTTAC |
Latest revision as of 02:57, 22 October 2009
Description | Oligo Name | Sequence | Length |
---|---|---|---|
amplifies first half of E. coli α-galactosidase gene adds SalI site (fwd) | HI-SalI-F | CATGTCGACATGATGTCTGCACCCAAAATT | 30 |
amplifies first half of α-galactosidase gene adds BamHI site (rev) | HI-BamHI-attP-R | ATGGGATCCCCCCCAACTGAGAGAACTCAAAGGTTACCCCAGTTGGGGGTAACGCAGCGTAGCTGG | 66 |
amplifies second half of α-galactosidase gene, adds KpnI site (fwd) | H2-KpnI-attP-F | CATGGTACCCCCCCAACTGAGAGAACTCAAAGGTTACCCCAGTTGGGGGCGTTTTACCTGGAGCTGG | 67 |
amplifies second half of α-galactosidase gene, adds XbaI site (rev) | H2-XbaI-R | ATGTCTAGAGGTCAGCAGCGTCTGT | 25 |
Amplifes any biobrick, adds EcoRV end for ligation into EcoRV blunt ends (fwd) | Prefix-EcoRV-F | ATCGAATTCGCGGCCGCTTCTAG | 23 |
Amplifes any biobrick, adds EcoRV end for ligation into EcoRV blunt ends (rev) | Suffix-EcoRV-R | ATCCTGCAGCGGCCGCTACTAGTA | 24 |
B0034 RBS primer, use with VF2 (fwd) | Prefix-RBS | GGCCGCTTCTAGAGAAAGAGG | 21 |
B0034 RBS primer, use with VR (rev) | Suffix-RBS | TTTCTCCTCTTTCTCTAGATGCGG | 24 |
ordered to add Plac and RBS to the cI ORF (project 2008) | Plac-RBS-F | GAATTCGCGGCCGCTTCTAGAGAATTGTGAGCGGATAACAATTGACATTGTGAGCGGATAA CAAGATACTGAGCACATACTAGAGAAAGAGGAGAAATACTAG | 103 |
amplifies ΦC31 int, adds RBS, Prefix, changes start codon to ATG, changes G to A maintaining glutamic acid codon and eliminating EcoRI site (fwd) | Int-BBa-F | GAATTCGCGGCCGCTTCTAGAGAAAGAGGAGAAATACTAGATGGACACGTACGCGGGTGCTT ACGACCGTCAGTCGCGCGAGCGCGAAAATTCG | 94 |
amplifies ΦC31 int, adds suffix, changes stop codon to double TAA (rev) | Int-DSC-R | CTGCAGCGGCCGCTACTAGTATTATTACGCCGCTACGTCTTCCGTG | 46 |
amplifies T7 Gene 6 exonuclease, adds ATG start codon, adds BBa prefix (fwd2) | T7gene6-F2 | GAATTCGCGGCCGCTTCTAGATGGCACTTCTTGACCTTAAAC | 42 |
does not add standard start codon (fwd) | T7gene6-F | CAATGGAATTCGCGGCCGCTTCTAGATGGCACTTCTTGACCTTAAA | 46 |
amplifies T7 Gene 6 exonuclease, adds double TAA stop codon, adds BBa suffix (rev2) | T7G6-R2 | CTGCAGCGGCCGCTACTAGTATTATTACGGTCTCCACAGGTAAATCTC | 48 |
does not add standard stop codon (rev) | T7gene6-R | CAATTTCTGCAGCGGCCGCTACTAGTACTACGGTCTCCACAGG | 43 |
amplifies T7 G6 makes product for cross over PCR with pmeI cross over PCR product | T7g6-RXO-RBS | CTAGTATTTCTCCTCTTTCTCTAGTATTATTACGGTCTCCACAGGTAAATCTC | 53 |
Amplifies T7 gene 5 exonuclease, a 3′-5′ exonuclease, adds BBa prefix (fwd) | T7gene5-F | CAATGGAATTCGCGGCCGCTTCTAGATGATCGTTTCTGACATCGAA | 46 |
Amplifies T7 gene 5 exonuclease, a 3′-5′ exonuclease, adds BBa suffix (rev) | T7gene5-R | CAATTTCTGCAGCGGCCGCTACTAGTATCAGTGGCAAATCGCCCAATTAGG | 51 |
amplifies phbA (fwd) | phbA-F | GAATTCGCGGCCGCTTCTAGATGAGCAATCCCTCGATCG | 39 |
amplifies phbA, non standard stop codon (rev) | phbB-R | CTGCAGCGGCCGCTACTAGTATTACAGGCGTTCCACGCACATGG | 44 |
amplifies phbB (fwd) | phbB-F | GAATTCGCGGCCGCTTCTAGATGAGCAGGGTAGCACTGG | 39 |
amplifies phbB, non standard stop codon (rev) | phbB-R | CTGCAGCGGCCGCTACTAGTA TCAGGCGAAGTACTGGCCGC | 41 |
amplifies phbC (fwd) | phbC-F | GAATTCGCGGCCGCTTCTAGATGACAGCAGAGAAGGCTGAGG | 42 |
amplifies phbC, non standard stop codon (rev) | phbC-R | CTGCAGCGGCCGCTACTAGTATCAGGCGCGCACGCGCACGTAGG | 44 |
amplifes RFP with [http://partsregistry.org/Part:BBa_J23118 BBa_J23118] constitutive promoter ([http://partsregistry.org/Part:BBa_K093012 BBa_K093012]) anneals to promoter sequence (fwd) | RFP-BamHI-F | CATGGATCCTTGACGGCTAGCTCAGTC | 27 |
anneals to RFP ORF sequence adds TT ([http://partsregistry.org/Part:BBa_B1002 BBa_B1002]) adds KpnI site, but don't use it to clone, the ORF contains other KpnI sites | RFP-KpnII-TT-R (rev) | ATGGGTACCAAAAAAAAACCCCGCCGAAGCGGGGTTTTTTTTTCTCTAGTAGCGATCTACACTAGCACTATCA | 73 |
amplifies pmeI from Pseudomonas mendocina NEB698, used to amplify synthetic ORF to add RBS (fwd) | PmeIR-F | GAATTCCGGCCGCTTCTAGAGAAAGAGGAGAAATACTAGATGACAACAAACTCCCCCTCAG | 61 |
amplifies pmeI from Pseudomonas mendocina NEB698, or our synthetic ORF (rev) | PmeIR-R | CTGCAGCGGCCGCTACTAGTATTATTAAACCGTACTTCGCCCCCG | 45 |
sense oligo for RecA-lexA hybrid promoter ([http://partsregistry.org/Part:BBa_K093000 BBa_K093000]) 5' EcoRI overhang (fwd) | recA-F | AATTCGCGGCCGCTTCTAGAGTACAAAACACTTGATACTGTATATATATACAGTATAATTGCTTCAACATA | 71 |
sense oligo for RecA lexA hybrid promoter ([http://partsregistry.org/Part:BBa_K093000 BBa_K093000]) 3' SpeI overhang (rev) | recA-R | CTAGTATGTTGAAGCAATTATACTGTATATATATACAGTATCAAGTGTTTTGTACTCTAGAAGCGGCCGCG | 71 |
amplifies RFP ORF adds RBS (fwd) | RFP-RBS-F | GAATTCCGGCCGCTTCTAGAGAAAGAGGAGAAATACTAGATGGCTTCCTCCGAAGACG | 58 |
amplifies RFP ORF, creates product for crossover PCR | RFP-RXO | GATGCCTGGCTCTAGTATTATTAAGCACCGGTGG | 34 |
Amplifies RK2 oriT adds MunI site (compatible end with EcoRI) (fwd) | RK2E-F | CATCAATTGCCGGCCAGCCTCGCAGAGCA | 29 |
Amplifies RK2 oriT adds EcoRI site, adds attB site in the forward orientation (rev) | RK2E-R | ATGGAATTCCGCGCCCGGGGAGCCCAAGGGCACGCCCTGGCACCCCGGGCAGGATAGGTGAAGT | 64 |
ampifies RK2 oriT adds PstI site, adds attB in the reverse orientation (fwd) | RK2-FP | CATCTGCAGCGCGCCCGGGGAGCCCAAGGGCACGCCCTGGCACCCCGGCCAGCCTCGCAGAGCA | 64 |
amplifes RK2 oriT adds Mph1103I site (compatible end with PstI) (rev) | RK2-RP | ATGATGCATCCGGGCAGGATAGGTGAAGT | 29 |
BBa sequencing/colony PCR primer (fwd) | VF2 | TGCCACCTGACGTCTAAGAA | 20 |
BBa sequencing/colony PCR primer (rev) | VR | ATTACCGCCTTTGAGTGAGC | 20 |
sequencing c31 int gene anneals internally | Int seq F | cgtgcaacgtcagcatggcg | |
sequenceing c31 int gene anneals internally | Int seq2 R | cgccatgctgacgttgcacg | |
sequenceing c31 int gene anneals internally | Int seq3 R | caccaccaccggattacgtc | |
was designed to fix an error in another PCR primer that was thrown out. Could still amplify biobricked c31 int | Int RBS | GAATTCGCGGCCGCTTCTAGAGAAAGAGGAGAAATACTAGATGGAC | |
sequencing primer for pKNG101 derived vectors (pFB9XXXX) | pKNG101 F | CTACATATCACAACGTGCGTG | |
sequencing primer for pKNG101 derived vectors (pFB9XXXX) | pKNG101 R | CTCCGTCTACTGTACGATACAC | |
linker+ for synthetic attP cloning into pKNG101 derived vectors | FB attP BamHI F | CTCAGTTGGGGGG | |
linker- for synthetic attP cloning into pKNG101 derived vectors | FB attP BamHI R | GATCCCCC | |
linker+ for synthetic attP cloning into pKNG101 derived vectors | FB attP SalI F | TCGACCCC | |
linker- for synthetic attP cloning into pKNG101 derived vectors | FB attP SalI R | CCCAGTTGGGGG | |
Bba linker+ attB | BBa attB EcoRI F | AATTCGCGGCCGCTTCTAGAG | |
Bba linker- attB | BBa attB EcoRI R | CACCCTCTAGAAGCGGCCGCG | |
Bba linker+ attB | BBa attB PstI F | CGCGTACTAGTAGCGGCCGCTGCA | |
Bba linker- attB | BBa attB PstI R | GCGGCCGCTACTAGTA | |
BBa linker+ attP | BBa attP EcoRI fwd | aattcgcggccgcttctagagCCC | 24 |
BBa linker- attP | BBa attP EcoRI rev | CCCAGTTGGGGctctagaagcggccgcg | |
BBa linker+ attP | BBa attP PstI fwd | CTCAGTTGGGGGGtactagtagcggccgctgca | 33 |
BBa linker- attP | BBa attP PstI rev | gcggccgctactagtaCCCC | 20 |
attB +strand | attB34 TG F | GGTGCCAGGGCGTGCCCTGGGGCTCCCCGGG | |
attB -strand | attB34 TG R | CGCGCCCGGGGAGCCCCAGGGCACGCCCTGG | |
attB +strand | attB34 CT F | GGTGCCAGGGCGTGCCCCTGGGCTCCCCGGG | |
attB -strand | attB34 CT R | CGCGCCCGGGGAGCCCAGGGGCACGCCCTGG | |
attB +strand | attB34 TT F | GGTGCCAGGGCGTGCCCTTGGGCTCCCCGGG | |
attB -strand | attB34 TT R | CGCGCCCGGGGAGCCCAAGGGCACGCCCTGG | |
attB +strand | attB 34 GA F | GGTGCCAGGGCGTGCCCGAGGGCTCCCCGGG | |
attB -strand | attB34 GA R | CGCGCCCGGGGAGCCCTCGGGCACGCCCTGG | |
attB +strand | attB34 AC F | GGTGCCAGGGCGTGCCCACGGGCTCCCCGGG | |
attB -strand | attB34 AC R | CGCGCCCGGGGAGCCCGTGGGCACGCCCTGG | |
attB +strand | attB34 GG F | GGTGCCAGGGCGTGCCCGGGGGCTCCCCGGG | |
attB -strand | attB34 GG R | CGCGCCCGGGGAGCCCCCGGGCACGCCCTGG | |
attP + strand | attP39 TG F | CAACTGGGGTAACCTTGGAGTTCT | |
attP - strand | attP39 TG R | CCAACTGAGAGAACTCCAAGGTTAC | |
attP + strand | attP39 CT F | CAACTGGGGTAACCTCTGAGTTCT | |
attP - strand | attP39 CT R | CCAACTGAGAGAACTCAGAGGTTAC | |
attP + strand | attP39 TT F | CAACTGGGGTAACCTTTGAGTTCT | |
attP - strand | attP39 TT R | CCAACTGAGAGAACTCAAAGGTTAC | |
attP + strand | attP39 GA F | CAACTGGGGTAACCTGAGAGTTCT | |
attP - strand | attP39 GA R | CCAACTGAGAGAACTCTCAGGTTAC | |
attP + strand | attP39 AC F | CAACTGGGGTAACCTACGAGTTCT | |
attP - strand | attP39 AC R | CCAACTGAGAGAACTCGTAGGTTAC | |
attP + strand | attP39 GG F | CAACTGGGGTAACCTGGGAGTTCT | |
attP - strand | attP39 GG R | CCAACTGAGAGAACTCCCAGGTTAC |