Team:Todai-Tokyo/Protocols/Restriction Enzyme Digest

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== Restriction Enzyme Digest Protocol ==
== Restriction Enzyme Digest Protocol ==
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* (1.5µl Restriction Enzyme 2, if applicable)
* (1.5µl Restriction Enzyme 2, if applicable)
* MilliQ up to 30ul
* MilliQ up to 30ul
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2. Incubate at 37ºC for 1h or more
2. Incubate at 37ºC for 1h or more

Latest revision as of 16:29, 20 October 2009

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Restriction Enzyme Digest Protocol

1. Mix the following in a eppendorf tube:

  • Xµl plasmid DNA (to a final concentration of 300ng/30ul)
  • 3µl 10 H buffer
  • 1.5µl Restriction Enzyme 1
  • (1.5µl Restriction Enzyme 2, if applicable)
  • MilliQ up to 30ul


2. Incubate at 37ºC for 1h or more