Team:VictoriaBC/Design

From 2009.igem.org

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   <a href="https://2009.igem.org/Team:VictoriaBC">HOME</a>
   <a href="https://2009.igem.org/Team:VictoriaBC">HOME</a>
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DESIGN<br>
 
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The final project consisted of a simplified biothermometer design.  Using the 32&deg;C ribothermometer linked to either RFP or GFP would entail a system that responded visually to differences in temperature.  There were two levels for this:  K235034 and K235035 used a lactose promoter and repressor to regulate RFP and GFP respectively; and K235036 and K235037 were directly coupled to RFP or GFP respectively.<br>
 
-
K235034<br>
 
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<table>
 
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<tr>
 
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            <td>K235034 (Chl) const. promoter+32oC temp. sensing RBS</td>
 
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            <td>K235033(Kan) const. promoter+32oC temp. sensing RBS</td>
 
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            <td><a href="http://partsregistry.org/Part:BBa_J04450">J04450</a>(Kan) Lac controlled RFP</td>
 
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            <td>P1010-Chl</td>
 
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            <td>
 
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            <p>No</p>
 
-
            </td>
 
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            <td>&nbsp;</td>
 
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            <td>&nbsp;</td>
 
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            <td>&nbsp;</td>
 
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            <td>&nbsp;</td>
 
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            <td>&nbsp;</td>
 
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        </tr>
 
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        <tr>
 
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            <td>K235035 (Chl) const. promoter+32oC temp. sensing RBS+ lactose promoter+RBS+GFP+stop</td>
 
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            <td>K235033(Kan) const. promoter+32oC temp. sensing RBS</td>
 
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            <td>K235032(Kan) lactose promoter+RBS+GFP+stop</td>
 
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            <td>P1010-Chl</td>
 
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            <td>No</td>
 
-
            <td>&nbsp;</td>
 
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            <td>&nbsp;</td>
 
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            <td>&nbsp;</td>
 
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            <td>&nbsp;</td>
 
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            <td>&nbsp;</td>
 
-
        </tr>
 
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        <tr>
 
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            <td>K235036 (Kan) const. promoter+32oC temp. sensing RBS+RFP+stop</td>
 
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            <td>K235030(Chl) const. promoter+32oC temp. sensing RBS</td>
 
-
            <td>K235005(Chl) RFP+stop</td>
 
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            <td>P1010-Kan</td>
 
-
            <td>Assembled</td>
 
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            <td>Colonies</td>
 
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            <td>Band</td>
 
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            <td>-20<sup>o</sup>C</td>
 
-
            <td>&nbsp;</td>
 
-
            <td>&nbsp;</td>
 
-
        </tr>
 
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        <tr>
 
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            <td>K235037 (Chl) const. promoter+32oC temp. sensing RBS+GFP+stop</td>
 
-
            <td>K235030(Kan) const. promoter+32oC temp. sensing RBS</td>
 
-
            <td><a href="http://partsregistry.org/Part:BBa_J04630">J04630</a>(Amp/Kan) GFP+stop</td>
 
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            <td>P1010-Chl</td>
 
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            <td>Assembled</td>
 
-
            <td>Colonies</td>
 
-
            <td>Band</td>
 
-
            <td>-20<sup>o</sup>C</td>
 
-
            <td>&nbsp;</td>
 
-
            <td>&nbsp;</td>
 
-
        </tr>
 
-
        <tr>
 
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            <td>K235038 (Chl) const. promoter+RBS</td>
 
-
            <td><a href="http://partsregistry.org/Part:BBa_J23102">J23102</a>(Amp) const. promoter</td>
 
-
            <td><a href="http://partsregistry.org/Part:BBa_B0034">B0034</a> (Amp) RBS</td>
 
-
            <td>P1010-Chl</td>
 
-
            <td>&nbsp;</td>
 
-
            <td>&nbsp;</td>
 
-
            <td>&nbsp;</td>
 
-
            <td>&nbsp;</td>
 
-
            <td>&nbsp;</td>
 
-
            <td>&nbsp;</td>
 
-
        </tr>
 
-
        <tr>
 
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            <td>K235038 (Kan) const. promoter+RBS</td>
 
-
            <td><a href="http://partsregistry.org/Part:BBa_J23102">J23102</a>(Amp) const. promoter</td>
 
-
            <td><a href="http://partsregistry.org/Part:BBa_B0034">B0034</a> (Amp) RBS</td>
 
-
            <td>P1010-Kan</td>
 
-
            <td>&nbsp;</td>
 
-
            <td>&nbsp;</td>
 
-
            <td>&nbsp;</td>
 
-
            <td>&nbsp;</td>
 
-
            <td>&nbsp;</td>
 
-
            <td>&nbsp;</td>
 
-
        </tr>
 
-
        <tr>
 
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            <td>K235039 (Kan) const. promoter+RBS+LacI repressor+LVA+stop</td>
 
-
            <td>K235038 (Chl) const. promoter+RBS</td>
 
-
            <td>K235031 (Chl) LacI repressor+LVA+stop</td>
 
-
            <td>P1010-Kan</td>
 
-
            <td>&nbsp;</td>
 
-
            <td>&nbsp;</td>
 
-
            <td>&nbsp;</td>
 
-
            <td>&nbsp;</td>
 
-
            <td>&nbsp;</td>
 
-
            <td>&nbsp;</td>
 
-
        </tr>
 
-
        <tr>
 
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            <td>K235040 (Chl) const. promoter+RBS+Lac controlled RFP</td>
 
-
            <td>K235039 (Kan) const. promoter+RBS</td>
 
-
            <td><a href="http://partsregistry.org/Part:BBa_J04450">J04450</a>(Kan) Lac controlled RFP</td>
 
-
            <td>P1010-Chl</td>
 
-
            <td>&nbsp;</td>
 
-
            <td>&nbsp;</td>
 
-
            <td>&nbsp;</td>
 
-
            <td>&nbsp;</td>
 
-
            <td>&nbsp;</td>
 
-
            <td>&nbsp;</td>
 
-
        </tr>
 
-
        <tr>
 
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            <td>K235041 (Chl) const. promoter+RBS+lactose promoter+RBS+GFP+stop</td>
 
-
            <td>K235039 (Kan) const. promoter+RBS</td>
 
-
            <td>K235032(Kan) lactose promoter+RBS+GFP+stop</td>
 
-
            <td>P1010-Chl</td>
 
-
            <td>&nbsp;</td>
 
-
            <td>&nbsp;</td>
 
-
            <td>&nbsp;</td>
 
-
            <td>&nbsp;</td>
 
-
            <td>&nbsp;</td>
 
-
            <td>&nbsp;</td>
 
-
        </tr>
 
-
        <tr>
 
-
            <td>K235042 (Kan) const. promoter+RBS+RFP+stop</td>
 
-
            <td>K235038 (Chl) const. promoter+RBS</td>
 
-
            <td>K235005(Chl) RFP+stop</td>
 
-
            <td>P1010-Kan</td>
 
-
            <td>&nbsp;</td>
 
-
            <td>&nbsp;</td>
 
-
            <td>&nbsp;</td>
 
-
            <td>&nbsp;</td>
 
-
            <td>&nbsp;</td>
 
-
            <td>&nbsp;</td>
 
-
        </tr>
 
-
        <tr>
 
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            <td>K235043 (Chl) const. promoter+RBS+GFP+stop</td>
 
-
            <td>K235038 (Kan) const. promoter+RBS</td>
 
-
            <td><a href="http://partsregistry.org/Part:BBa_J04630">J04630</a>(Amp/Kan) GFP+stop</td>
 
-
            <td>P1010-Chl</td>
 
-
            <td>&nbsp;</td>
 
-
            <td>&nbsp;</td>
 
-
            <td>&nbsp;</td>
 
-
            <td>&nbsp;</td>
 
-
            <td>&nbsp;</td>
 
-
            <td>&nbsp;</td>
 
-
        </tr>
 
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</table>
 
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 +
<td width="750" rowspan=30 bgcolor="#e4e4fe" valign="top">
 +
<p><b>DESIGN</b><br>
 +
The final project consisted of a simplified biothermometer design.  Using the 32&deg;C ribothermometer linked to either RFP or GFP would produce a system with a visual response to differences in temperature.  There were two levels for this:  K235034 and K235035 used a lactose promoter and repressor to regulate RFP and GFP respectively; and K235036 and K235037 were directly coupled to RFP or GFP respectively.<br>
 +
</p><br>
 +
<table>
 +
<tr>
 +
            <td>K235034 (Chl) const. promoter+32<sup>o</sup>C temp. sensing RBS</td>
 +
            <td>K235033(Kan) const. promoter+32<sup>o</sup>C temp. sensing RBS</td>
 +
            <td><a href="http://partsregistry.org/Part:BBa_J04450">J04450</a>(Kan) Lac controlled RFP</td>
 +
            <td>P1010-Chl</td>
 +
        </tr>
 +
        <tr>
 +
            <td>K235035 (Chl) const. promoter+32<sup>o</sup>C temp. sensing RBS+ lactose promoter+RBS+GFP+stop</td>
 +
            <td>K235033(Kan) const. promoter+32<sup>o</sup>C temp. sensing RBS</td>
 +
            <td>K235032(Kan) lactose promoter+RBS+GFP+stop</td>
 +
            <td>P1010-Chl</td>
 +
        </tr>
 +
        <tr>
 +
            <td>K235036 (Kan) const. promoter+32<sup>o</sup>C temp. sensing RBS+RFP+stop</td>
 +
            <td>K235030(Chl) const. promoter+32<sup>o</sup>C temp. sensing RBS</td>
 +
            <td>K235005(Chl) RFP+stop</td>
 +
            <td>P1010-Kan</td>
 +
        </tr>
 +
        <tr>
 +
            <td>K235037 (Chl) const. promoter+32<sup>o</sup>C temp. sensing RBS+GFP+stop</td>
 +
            <td>K235030(Kan) const. promoter+32<sup>o</sup>C temp. sensing RBS</td>
 +
            <td><a href="http://partsregistry.org/Part:BBa_J04630">J04630</a>(Amp/Kan) GFP+stop</td>
 +
            <td>P1010-Chl</td>
 +
        </tr>
 +
        <tr>
 +
            <td>K235038 (Chl) const. promoter+RBS</td>
 +
            <td><a href="http://partsregistry.org/Part:BBa_J23102">J23102</a>(Amp) const. promoter</td>
 +
            <td><a href="http://partsregistry.org/Part:BBa_B0034">B0034</a> (Amp) RBS</td>
 +
            <td>P1010-Chl</td>
 +
        </tr>
 +
        <tr>
 +
            <td>K235038 (Kan) const. promoter+RBS</td>
 +
            <td><a href="http://partsregistry.org/Part:BBa_J23102">J23102</a>(Amp) const. promoter</td>
 +
            <td><a href="http://partsregistry.org/Part:BBa_B0034">B0034</a> (Amp) RBS</td>
 +
            <td>P1010-Kan</td>
 +
        </tr>
 +
        <tr>
 +
            <td>K235039 (Kan) const. promoter+RBS+LacI repressor+LVA+stop</td>
 +
            <td>K235038 (Chl) const. promoter+RBS</td>
 +
            <td>K235031 (Chl) LacI repressor+LVA+stop</td>
 +
            <td>P1010-Kan</td>
 +
        </tr>
 +
        <tr>
 +
            <td>K235040 (Chl) const. promoter+RBS+Lac controlled RFP</td>
 +
            <td>K235039 (Kan) const. promoter+RBS</td>
 +
            <td><a href="http://partsregistry.org/Part:BBa_J04450">J04450</a>(Kan) Lac controlled RFP</td>
 +
            <td>P1010-Chl</td>
 +
        </tr>
 +
        <tr>
 +
            <td>K235041 (Chl) const. promoter+RBS+lactose promoter+RBS+GFP+stop</td>
 +
            <td>K235039 (Kan) const. promoter+RBS</td>
 +
            <td>K235032(Kan) lactose promoter+RBS+GFP+stop</td>
 +
            <td>P1010-Chl</td>
 +
        </tr>
 +
        <tr>
 +
            <td>K235042 (Kan) const. promoter+RBS+RFP+stop</td>
 +
            <td>K235038 (Chl) const. promoter+RBS</td>
 +
            <td>K235005(Chl) RFP+stop</td>
 +
            <td>P1010-Kan</td>
 +
        </tr>
 +
        <tr>
 +
            <td>K235043 (Chl) const. promoter+RBS+GFP+stop</td>
 +
            <td>K235038 (Kan) const. promoter+RBS</td>
 +
            <td><a href="http://partsregistry.org/Part:BBa_J04630">J04630</a>(Amp/Kan) GFP+stop</td>
 +
            <td>P1010-Chl</td>
 +
        </tr>
 +
</table>
 +
</td>
 +
</tr>

Latest revision as of 03:44, 22 October 2009


HOME
PROJECT
OVERVIEW
DESIGN
TESTING
RESULTS
CONCLUSIONS
TEAM
LAB NOTEBOOK
LAB PROTOCOLS
PARTS

DESIGN
The final project consisted of a simplified biothermometer design. Using the 32°C ribothermometer linked to either RFP or GFP would produce a system with a visual response to differences in temperature. There were two levels for this: K235034 and K235035 used a lactose promoter and repressor to regulate RFP and GFP respectively; and K235036 and K235037 were directly coupled to RFP or GFP respectively.


K235034 (Chl) const. promoter+32oC temp. sensing RBS K235033(Kan) const. promoter+32oC temp. sensing RBS J04450(Kan) Lac controlled RFP P1010-Chl
K235035 (Chl) const. promoter+32oC temp. sensing RBS+ lactose promoter+RBS+GFP+stop K235033(Kan) const. promoter+32oC temp. sensing RBS K235032(Kan) lactose promoter+RBS+GFP+stop P1010-Chl
K235036 (Kan) const. promoter+32oC temp. sensing RBS+RFP+stop K235030(Chl) const. promoter+32oC temp. sensing RBS K235005(Chl) RFP+stop P1010-Kan
K235037 (Chl) const. promoter+32oC temp. sensing RBS+GFP+stop K235030(Kan) const. promoter+32oC temp. sensing RBS J04630(Amp/Kan) GFP+stop P1010-Chl
K235038 (Chl) const. promoter+RBS J23102(Amp) const. promoter B0034 (Amp) RBS P1010-Chl
K235038 (Kan) const. promoter+RBS J23102(Amp) const. promoter B0034 (Amp) RBS P1010-Kan
K235039 (Kan) const. promoter+RBS+LacI repressor+LVA+stop K235038 (Chl) const. promoter+RBS K235031 (Chl) LacI repressor+LVA+stop P1010-Kan
K235040 (Chl) const. promoter+RBS+Lac controlled RFP K235039 (Kan) const. promoter+RBS J04450(Kan) Lac controlled RFP P1010-Chl
K235041 (Chl) const. promoter+RBS+lactose promoter+RBS+GFP+stop K235039 (Kan) const. promoter+RBS K235032(Kan) lactose promoter+RBS+GFP+stop P1010-Chl
K235042 (Kan) const. promoter+RBS+RFP+stop K235038 (Chl) const. promoter+RBS K235005(Chl) RFP+stop P1010-Kan
K235043 (Chl) const. promoter+RBS+GFP+stop K235038 (Kan) const. promoter+RBS J04630(Amp/Kan) GFP+stop P1010-Chl

Retrieved from "http://2009.igem.org/Team:VictoriaBC/Design"