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Team:VictoriaBC/Design
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| - | <a href="https://2009.igem.org/Team:VictoriaBC/ | + | <a href="https://2009.igem.org/Team:VictoriaBC/Testing">TESTING</a> |
</td> | </td> | ||
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<td width="750" rowspan=30 bgcolor="#e4e4fe" valign="top"> | <td width="750" rowspan=30 bgcolor="#e4e4fe" valign="top"> | ||
| - | DESIGN<br> | + | <p><b>DESIGN</b><br> |
| - | The final project consisted of a simplified biothermometer design. Using the 32°C ribothermometer linked to either RFP or GFP would | + | The final project consisted of a simplified biothermometer design. Using the 32°C ribothermometer linked to either RFP or GFP would produce a system with a visual response to differences in temperature. There were two levels for this: K235034 and K235035 used a lactose promoter and repressor to regulate RFP and GFP respectively; and K235036 and K235037 were directly coupled to RFP or GFP respectively.<br> |
| - | + | </p><br> | |
<table> | <table> | ||
<tr> | <tr> | ||
| - | <td>K235034 (Chl) const. promoter+ | + | <td>K235034 (Chl) const. promoter+32<sup>o</sup>C temp. sensing RBS</td> |
| - | <td>K235033(Kan) const. promoter+ | + | <td>K235033(Kan) const. promoter+32<sup>o</sup>C temp. sensing RBS</td> |
<td><a href="http://partsregistry.org/Part:BBa_J04450">J04450</a>(Kan) Lac controlled RFP</td> | <td><a href="http://partsregistry.org/Part:BBa_J04450">J04450</a>(Kan) Lac controlled RFP</td> | ||
<td>P1010-Chl</td> | <td>P1010-Chl</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td>K235035 (Chl) const. promoter+ | + | <td>K235035 (Chl) const. promoter+32<sup>o</sup>C temp. sensing RBS+ lactose promoter+RBS+GFP+stop</td> |
| - | <td>K235033(Kan) const. promoter+ | + | <td>K235033(Kan) const. promoter+32<sup>o</sup>C temp. sensing RBS</td> |
<td>K235032(Kan) lactose promoter+RBS+GFP+stop</td> | <td>K235032(Kan) lactose promoter+RBS+GFP+stop</td> | ||
<td>P1010-Chl</td> | <td>P1010-Chl</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td>K235036 (Kan) const. promoter+ | + | <td>K235036 (Kan) const. promoter+32<sup>o</sup>C temp. sensing RBS+RFP+stop</td> |
| - | <td>K235030(Chl) const. promoter+ | + | <td>K235030(Chl) const. promoter+32<sup>o</sup>C temp. sensing RBS</td> |
<td>K235005(Chl) RFP+stop</td> | <td>K235005(Chl) RFP+stop</td> | ||
<td>P1010-Kan</td> | <td>P1010-Kan</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td>K235037 (Chl) const. promoter+ | + | <td>K235037 (Chl) const. promoter+32<sup>o</sup>C temp. sensing RBS+GFP+stop</td> |
| - | <td>K235030(Kan) const. promoter+ | + | <td>K235030(Kan) const. promoter+32<sup>o</sup>C temp. sensing RBS</td> |
<td><a href="http://partsregistry.org/Part:BBa_J04630">J04630</a>(Amp/Kan) GFP+stop</td> | <td><a href="http://partsregistry.org/Part:BBa_J04630">J04630</a>(Amp/Kan) GFP+stop</td> | ||
<td>P1010-Chl</td> | <td>P1010-Chl</td> | ||
Latest revision as of 03:44, 22 October 2009
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DESIGN
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