Team:Heidelberg/Notebook measure/NotebookRT
From 2009.igem.org
(Difference between revisions)
Chenchenzhu (Talk | contribs) (→8-24-2009) |
Chenchenzhu (Talk | contribs) (→9-24-2009) |
||
(11 intermediate revisions not shown) | |||
Line 1: | Line 1: | ||
- | =''' | + | __NOTOC__ |
+ | {{Template_HD_3}} | ||
+ | <html> | ||
+ | <style = "text/css"> | ||
+ | #body {margin: 0} | ||
+ | </style> | ||
+ | <body id="project"></body></html> | ||
+ | |||
+ | {| valign="top" border="0" | ||
+ | |width="900px" style="padding: 0 15px 15px 20px; background-color:#ede8e2"| | ||
+ | __NOTOC__ | ||
+ | == '''Real-time RT-PCR''' == | ||
+ | |||
== Contents == | == Contents == | ||
- | {| class="wikitable centered" border="2" rules="rows" width=" | + | {| class="wikitable centered" border="2" rules="rows" width="900px" style="border-color:white;" |
|- | |- | ||
- | + | ! Week !! colspan="7" |Days | |
+ | |- | ||
+ | |style="text-align:center"| 35 | ||
+ | |style="text-align:center"| [[Team:Heidelberg/Notebook_measure/NotebookRT#8-24-2009|8-24-2009]] | ||
+ | |style="text-align:center"| - | ||
+ | |style="text-align:center"| [[Team:Heidelberg/Notebook_measure/NotebookRT#8-26-2009|8-26-2009]] | ||
+ | |style="text-align:center"| [[Team:Heidelberg/Notebook_measure/NotebookRT#8-27-2009|8-27-2009]] | ||
+ | |style="text-align:center"| - | ||
+ | |style="text-align:center"| - | ||
+ | |style="text-align:center"| - | ||
+ | |- | ||
+ | |style="text-align:center"| 36 | ||
+ | |style="text-align:center"| [[Team:Heidelberg/Notebook_measure/NotebookRT#8-31-2009|8-31-2009]] | ||
+ | |style="text-align:center"| - | ||
+ | |style="text-align:center"| [[Team:Heidelberg/Notebook_measure/NotebookRT#9-02-2009|9-02-2009]] | ||
+ | |style="text-align:center"| [[Team:Heidelberg/Notebook_measure/NotebookRT#9-03-2009|9-03-2009]] | ||
+ | |style="text-align:center"| [[Team:Heidelberg/Notebook_measure/NotebookRT#9-04-2009|9-04-2009]] | ||
+ | |style="text-align:center"| - | ||
+ | |style="text-align:center"| - | ||
+ | |- | ||
+ | |style="text-align:center"| 37 | ||
+ | |style="text-align:center"| - | ||
+ | |style="text-align:center"| - | ||
+ | |style="text-align:center"| - | ||
+ | |style="text-align:center"| [[Team:Heidelberg/Notebook_measure/NotebookRT#9-10-2009|9-10-2009]] | ||
+ | |style="text-align:center"| - | ||
+ | |style="text-align:center"| - | ||
+ | |style="text-align:center"| - | ||
+ | |- | ||
+ | |style="text-align:center"| 39 | ||
+ | |style="text-align:center"| [[Team:Heidelberg/Notebook_measure/NotebookRT#9-21-2009|9-21-2009]] | ||
+ | |style="text-align:center"| - | ||
+ | |style="text-align:center"| - | ||
+ | |style="text-align:center"| [[Team:Heidelberg/Notebook_measure/NotebookRT#9-24-2009|9-24-2009]] | ||
+ | |style="text-align:center"| [[Team:Heidelberg/Notebook_measure/NotebookRT#9-25-2009|9-25-2009]] | ||
+ | |style="text-align:center"| - | ||
+ | |style="text-align:center"| - | ||
+ | |- | ||
+ | |style="text-align:center"| 40 | ||
+ | |style="text-align:center"| - | ||
+ | |style="text-align:center"| - | ||
+ | |style="text-align:center"| [[Team:Heidelberg/Notebook_measure/NotebookRT#9-30-2009|9-30-2009]] | ||
+ | |style="text-align:center"| - | ||
+ | |style="text-align:center"| [[Team:Heidelberg/Notebook_measure/NotebookRT#10-02-2009|10-02-2009]] | ||
+ | |style="text-align:center"| - | ||
+ | |style="text-align:center"| - | ||
+ | |- | ||
+ | |} | ||
+ | |||
== 8-24-2009== | == 8-24-2009== | ||
* Design primers and probes for housekeeping genes (GAPDH, G6PD, 18s rRNA, TUBG2, ATCB) and fluorescent proteins (eGFP, mCherry). | * Design primers and probes for housekeeping genes (GAPDH, G6PD, 18s rRNA, TUBG2, ATCB) and fluorescent proteins (eGFP, mCherry). | ||
Line 46: | Line 106: | ||
== 9-04-2009== | == 9-04-2009== | ||
*RNA extration of HeLa transfected with CMV and JeT. | *RNA extration of HeLa transfected with CMV and JeT. | ||
+ | **yield: 3.7 µg/1.5*10<sup>5</sup> cells | ||
+ | [[Image:HD09_RNA_GEL1.png|thumb|left|900px|<div style="text-align:justify;">'''Gel electrophoresis of RNA extracted from HeLa cells.''' 1% agarose gel, 100V, 60 min. </div>]] | ||
*Real-time RT PCR | *Real-time RT PCR | ||
- | **result:mRNA(CMV):mRNA(JeT)=1. | + | **result:mRNA(CMV):mRNA(JeT)=1.73:1 |
== 9-10-2009== | == 9-10-2009== | ||
Line 66: | Line 128: | ||
*Dilution: to 20 ng/µl | *Dilution: to 20 ng/µl | ||
*gel electrophoresis: positive | *gel electrophoresis: positive | ||
+ | [[Image:HD09_RNA_GEL2.png|thumb|left|900px|<div style="text-align:justify;">'''Gel electrophoresis of RNA extracted from HeLa cells.''' 1% agarose gel, 100V, 60 min. </div>]] | ||
== 9-25-2009== | == 9-25-2009== | ||
Line 77: | Line 140: | ||
**Dilute the products to 20 ng/µl | **Dilute the products to 20 ng/µl | ||
*Real-time RT PCR for CMV and JeT (50h) | *Real-time RT PCR for CMV and JeT (50h) | ||
+ | |||
+ | |} |
Latest revision as of 19:24, 21 October 2009
Real-time RT-PCRContents
8-24-2009
8-26-2009
8-31-2009
9-02-2009
9-03-2009
9-04-2009
9-10-2009
9-21-2009
9-24-2009
9-25-2009
9-30-2009
10-02-2009
|