Team:UNIPV-Pavia/Methods Materials/XGal

From 2009.igem.org

(Difference between revisions)
(→X-Gal staining protocol for beta galactosidase (blue/white screening))
(→X-Gal staining protocol for beta galactosidase (blue/white screening))
 
(8 intermediate revisions not shown)
Line 1: Line 1:
-
{{UNIPV-Pavia/StyleCss}}
 
<html>
<html>
<head>
<head>
-
<link rel="stylesheet" type="text/css" href="http://bioinfo.unipv.it/igem/templates/SpryAssets/SpryMenuBar.js" title="Stile"/>
+
 
-
</head>
+
-
<body>
+
<table width="100%">
<table width="100%">
   <tr>
   <tr>
<td align="center">
<td align="center">
-
<img width="100%" height="180px" src="https://static.igem.org/mediawiki/2009/c/c2/UNIPV_title_MandM.jpg" ></img>
+
<img width="100%" height="180px" src="https://static.igem.org/mediawiki/2009/d/de/UNIPV_title_M%26M.jpg" ></img>
</td>
</td>
   </tr>
   </tr>
</table>
</table>
-
</body>
+
 
</html>
</html>
Line 25: Line 22:
     <font face="Pristina" size="50" ><b>Protocols</b></font>
     <font face="Pristina" size="50" ><b>Protocols</b></font>
     </td>
     </td>
 +
    <td width="50px"></td>
</html>
</html>
   </tr>
   </tr>
Line 43: Line 41:
*Result: blue colonies express LacZ, while white colonies don't.
*Result: blue colonies express LacZ, while white colonies don't.
<br><br><br><br><br><br><br><br>
<br><br><br><br><br><br><br><br>
-
<br></td><td class='beuta' align='center' valign='bottom' width='300px'>
+
<br></td><td align='center' valign='top' width='300px'>
-
<div align='center'>
+
 
<table border='1'>
<table border='1'>
 +
<tr bgcolor='yellow'><td>INGREDIENTS:
 +
</td></tr>
<tr><td>
<tr><td>
-
*'''40 mg/ml X-gal in DMF'''
+
<font class='label'>
-
*'''SOC medium'''
+
'''40 mg/ml X-gal in DMF'''<hr color='white' width='70%'>
-
*'''Pre-warmed LB agar plate'''
+
'''SOC medium'''<hr color='white' width='70%'>
-
*'''Face mask'''
+
'''Pre-warmed LB agar plate'''<hr color='white' width='70%'>
-
<br>
+
'''Face mask'''</font>
-
</td></tr></table></div><br><br><br><br>
+
</td></tr></table><br><br><br><br>
</td></tr></table>
</td></tr></table>

Latest revision as of 10:27, 21 October 2009

EthanolPVanimation.gif



Protocols


X-Gal staining protocol for beta galactosidase (blue/white screening)

(Estimated time: 20 min)

  • The principle is that X-Gal (5-bromo-4-chloro-3-indolyl-b-D-galactopyranoside) turns blue when reacts with beta-galactosidase.
  • Mix 20 ul X-Gal 20 mg/ml and 80 ul SOC and spread on LB+amp plates (X-Gal and DMF are toxic, use face-mask for your safety!!! X-Gal is light-sensitive, remember to keep it in the dark, when possible)
  • Incubate with 200 ul bacteria
  • Result: blue colonies express LacZ, while white colonies don't.










INGREDIENTS:

40 mg/ml X-gal in DMF
SOC medium
Pre-warmed LB agar plate

Face mask





Retrieved from "http://2009.igem.org/Team:UNIPV-Pavia/Methods_Materials/XGal"