Team:UNIPV-Pavia/Methods Materials/Glycerol stock

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     <font face="Pristina" size="50" ><b>Protocols</b></font>
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*Mix 750 ul from the 5 ml sample of overnight incubated bacteria with 250 ul of 80% glycerol
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*Mix 750 ul from the 5 ml sample of the incubated bacteria with 250 ul of 80% glycerol
*Leave at -20°C for one day
*Leave at -20°C for one day
*Move to -80°C the day after
*Move to -80°C the day after
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<tr bgcolor='yellow'><td>INGREDIENTS:
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*'''o/n incubated bacteria'''
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*'''80% glycerol'''
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'''o/n or in exp phase bacterial culture'''<hr color='white' width='70%'>
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'''80% glycerol'''</font>
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Latest revision as of 10:27, 21 October 2009

EthanolPVanimation.gif



Protocols


Glycerol stocks

(estimated time: 15 min + freezing time)

  • Mix 750 ul from the 5 ml sample of the incubated bacteria with 250 ul of 80% glycerol
  • Leave at -20°C for one day
  • Move to -80°C the day after

















INGREDIENTS:

o/n or in exp phase bacterial culture

80% glycerol





Retrieved from "http://2009.igem.org/Team:UNIPV-Pavia/Methods_Materials/Glycerol_stock"