Team:USTC/Modeling/Model-3

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== '''pConX<sub>1</sub>+tetR+pConX<sub>2</sub>+LuxR+pLux/Tet+GFP''' ==
== '''pConX<sub>1</sub>+tetR+pConX<sub>2</sub>+LuxR+pLux/Tet+GFP''' ==
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'''Parts:'''  K176066 K176067 K176070 K176071 K176074 K176075 K176078 K176079 K176136 K176138 K176140 K176142 K176144 K176176 K176178 K176180 K176182 K176184
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'''Parts:'''   
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<partinfo>K176066</partinfo> <partinfo>K176066 DeepComponents</partinfo>
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<partinfo>K176067</partinfo> <partinfo>K176070</partinfo> <partinfo>K176071</partinfo> <partinfo>K176074</partinfo> <partinfo>K176075</partinfo> <partinfo>K176078</partinfo> <partinfo>K176079</partinfo> <partinfo>K176136</partinfo> <partinfo>K176138</partinfo> <partinfo>K176140</partinfo> <partinfo>K176142</partinfo> <partinfo>K176144</partinfo> <partinfo>K176176</partinfo> <partinfo>K176178</partinfo> <partinfo>K176180</partinfo> <partinfo>K176182</partinfo> <partinfo>K176184</partinfo>
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[[Image:model-3-1.jpg|700px]]
[[Image:model-3-1.jpg|700px]]
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== '''Simplified Model-3''' ==
== '''Simplified Model-3''' ==
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To get the datails of the model-processing, click [[Team:USTC/Modeling/Model-2|'''Simplified Model-2]]
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We employed an ODE model. We defined the input to be AHL<sub>out</sub> the and the output to be the synthesis rate of mature GFP.
We employed an ODE model. We defined the input to be AHL<sub>out</sub> the and the output to be the synthesis rate of mature GFP.
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[[Image:s-m-2-1.jpg|550px]]
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[[Image:s-m-3-1.jpg|700px]]
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To simplify the model, the (Recation S3-3), (Recation S3-4), (Recation S3-5),(Recation S3-13) and (Recation S3-14) were considered to be able to get balance in a very shortly time.
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The modeling is similar to the [[Team:USTC/Modeling/Model-2|'''Simplified Model-2]]
 
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To simplify the model, the (Recation S2-4), (Recation S2-5) and (Recation S2-4) were considered to be able to get balance in a very shortly time.
 
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The values of 4.8E-3 for rmG, 4.8E-3 for a and 0.4 for pG were got from the F2620, and the value for d of 0.00368 based on the measurement of the OD.
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[[Image:s-m-3-3.jpg|800px]]
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[[Image:s-m-2-2.jpg|900px]]
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Conbining the data from our measurements and the parameters estimated in [[Team:USTC/Modeling/Model-2|'''Simplified Model-2''']],  we could be able to get the parameter kkk, k<sub>11</sub>, k<sub>12</sub>, k<sub>13</sub>, k<sub>14</sub>, k<sub>15</sub>, k<sub>16</sub>.

Latest revision as of 03:49, 22 October 2009

USTC
Home Team Project Modeling Parts Standard & Protocol Software Tool Human Practice Notebook

Team:USTC/Modeling/Model-3

Contents


pConX1+tetR+pConX2+LuxR+pLux/Tet+GFP

Parts:



Model-3-1.jpg

Model-3-2.jpg

Simplified Model-3

To get the datails of the model-processing, click Simplified Model-2

We employed an ODE model. We defined the input to be AHLout the and the output to be the synthesis rate of mature GFP.

S-m-3-1.jpg

To simplify the model, the (Recation S3-3), (Recation S3-4), (Recation S3-5),(Recation S3-13) and (Recation S3-14) were considered to be able to get balance in a very shortly time.


S-m-3-3.jpg

Conbining the data from our measurements and the parameters estimated in Simplified Model-2, we could be able to get the parameter kkk, k11, k12, k13, k14, k15, k16.

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