Team:Wash U/Calendar-Home/2 July 2009

From 2009.igem.org

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-Ran gels on digested plasmids from yesterday (7/1)
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{{WashUback}}
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== Thursday July 2, 2009 ==
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-Ran gels to see if yesterday's (7/1) digestions were successful <br>
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[https://static.igem.org/mediawiki/2009/a/a4/DigestGel1_7-2.jpg Gel 1] <br>
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[https://static.igem.org/mediawiki/2009/e/e5/DigestGel2_7-2.jpg Gel 2] <br>
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[https://static.igem.org/mediawiki/2009/9/98/Smallgel7-2.jpg Gel 3]
-Spun down cells from yesterday's overnight cultures, for minipreps
-Spun down cells from yesterday's overnight cultures, for minipreps
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Miniprep #3
Miniprep #3
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<table>
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<tr>
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<td>
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1) BBa_I15010  red light sensor
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2) pSB1K3 
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3) pSB1T3 
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4) pRKCBC3 
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5) pSB1AC3 
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6) pSB1A3 
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7) pucHS27 
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8) pSB1AT3 
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9) BBa_R0082  OmpC promoter 
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10) BBa_E0240  GFP 
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11) BBa_B0034  RBS 
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12) BBa_B0015  terminator 
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<td>
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9.0ng/ul
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37.0ng/ul
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74.0ng/ul
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33.0ng/ul
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52.0ng/ul
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52.0ng/ul
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1) BBa_I15010  red light sensor
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77.5ng/ul
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2) pSB1K3
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81.5ng/ul
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3) pSB1T3
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11.5ng/ul
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4) pRKCBC3
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31.5ng/ul
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5) pSB1ac3
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30.5ng/ul
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6) pSB1A3
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30.5ng/ul
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</table>
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7) pucHS27
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<pre>
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*Measured again with EtOH wipe after each sample, 7-15
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</pre>
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8) pSB1AT3
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<div style="text-align: right;">
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<div style="float: left;">
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[[Team:Wash_U/Calendar-Home/1 July 2009|Previous entry]]</div>
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[[Team:Wash_U/Calendar-Home/3 July 2009|Next entry]]</div>
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<div style="clear: left;"></div>
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9) BBa_R0082  OmpC promoter
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<center>[[Team:Wash U/Notebook|Back to Calendar]]</center>
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10) BBa_E0240  GFP
 
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11) BBa_B0034  RBS
 
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12) BBa_B0015  terminator
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{{WashUbottom}}

Latest revision as of 21:13, 15 July 2009



Thursday July 2, 2009

-Ran gels to see if yesterday's (7/1) digestions were successful
Gel 1
Gel 2
Gel 3

-Spun down cells from yesterday's overnight cultures, for minipreps


Miniprep #3

1) BBa_I15010 red light sensor

2) pSB1K3

3) pSB1T3

4) pRKCBC3

5) pSB1AC3

6) pSB1A3

7) pucHS27

8) pSB1AT3

9) BBa_R0082 OmpC promoter

10) BBa_E0240 GFP

11) BBa_B0034 RBS

12) BBa_B0015 terminator

9.0ng/ul

37.0ng/ul

74.0ng/ul

33.0ng/ul

52.0ng/ul

52.0ng/ul

77.5ng/ul

81.5ng/ul

11.5ng/ul

31.5ng/ul

30.5ng/ul

30.5ng/ul 

*Measured again with EtOH wipe after each sample, 7-15
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Next entry
Back to Calendar


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