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Uppsala-Sweden/15 October 2009
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{{Uppsala-Sweden Template}} | {{Uppsala-Sweden Template}} | ||
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| + | ==Triparental mating== | ||
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| + | The transfer of PRKRYT to cynecosystis were started today awaiting to be continued tomorrow. | ||
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| + | ==Evaluating transformations== | ||
The colonies that were picked yesterday were evaluated on a gel. | The colonies that were picked yesterday were evaluated on a gel. | ||
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(From left) | (From left) | ||
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1. Fermentas 1kb ladder | 1. Fermentas 1kb ladder | ||
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2-6. PRPRYT(AC) 1-5 | 2-6. PRPRYT(AC) 1-5 | ||
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7-11. PRKRYT(XAK) 1-5 | 7-11. PRKRYT(XAK) 1-5 | ||
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12. Fermentas 1kb ladder | 12. Fermentas 1kb ladder | ||
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(from left) | (from left) | ||
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1. Fermentas 1kb ladder | 1. Fermentas 1kb ladder | ||
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2-6. PRPRAT(XAK) 1-5 | 2-6. PRPRAT(XAK) 1-5 | ||
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7-11. PE1B3T(XAK) 1-5 | 7-11. PE1B3T(XAK) 1-5 | ||
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12. Fermentas 1kb ladder | 12. Fermentas 1kb ladder | ||
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Inoculations of PRPRYT 2 & 4 and PRKRYT 4. | Inoculations of PRPRYT 2 & 4 and PRKRYT 4. | ||
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New colonies were pciked for the failing ones but with poor result again, as suspected. Only one colony for PRPRAT had the right length, and this one were inoculated as well. | New colonies were pciked for the failing ones but with poor result again, as suspected. Only one colony for PRPRAT had the right length, and this one were inoculated as well. | ||
Latest revision as of 18:10, 21 October 2009
Triparental mating
The transfer of PRKRYT to cynecosystis were started today awaiting to be continued tomorrow.
Evaluating transformations
The colonies that were picked yesterday were evaluated on a gel.
(From left)
1. Fermentas 1kb ladder
2-6. PRPRYT(AC) 1-5
7-11. PRKRYT(XAK) 1-5
12. Fermentas 1kb ladder
(from left)
1. Fermentas 1kb ladder
2-6. PRPRAT(XAK) 1-5
7-11. PE1B3T(XAK) 1-5
12. Fermentas 1kb ladder
Inoculations of PRPRYT 2 & 4 and PRKRYT 4.
New colonies were pciked for the failing ones but with poor result again, as suspected. Only one colony for PRPRAT had the right length, and this one were inoculated as well.
