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Team:ArtScienceBangalore/Notebook/Week Four
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===June 9th=== | ===June 9th=== | ||
| - | + | [http://hackteria.org/wiki/index.php/The_June_9th_Image_Gallery The June 9th Image Gallery] | |
Images of the various results attained: | Images of the various results attained: | ||
Latest revision as of 21:20, 21 October 2009
Week Four
June 8th- 17th
What are we trying to do?
We want the bacteria to lyse, to kill itself from the inside.
Well then, to do that we had to get a lysis gene in it. The thing is, with the lysis gene needs to be turned on by this specific substance called a promoter
There are two kinds of promoters, ones that make the gene they're attached to , be 'on' all the time. Then, you have ones that turn on when you want them to, in our case, when you add a mystery enzyme.
Our Step-wise Process:
How we tried to do this:
[http://hackteria.org/wiki/index.php/Step_1_-_Taking_dry_DNA_from_wells Step 1 - Taking dry DNA from wells]
[http://hackteria.org/wiki/index.php/Step_2_-_Transforming_competent_cells Step 2 - Transforming competent cells]
Step 3 - Picking a single colony.
Step 4 - Inoculating broth with Ampicillin-R (an antibiotic) and letting it grow for 18 hours.
Step 5 - Using the resulting culture for [http://hackteria.org/wiki/index.php/Mini-prep. mini-prep.] - a process used to purify plasmids and yields clean, usable DNA.
Step 6 - [http://hackteria.org/wiki/index.php/Digesting_the_DNA Digesting the DNA]
Step 7 - [http://hackteria.org/wiki/index.php/Gel_Electrophoresis Gel Electrophoresis]
Step 8- [http://hackteria.org/wiki/index.php/Ligation Ligation]
Step 9- Transformation and Inoculation
June 9th
[http://hackteria.org/wiki/index.php/The_June_9th_Image_Gallery The June 9th Image Gallery]
Images of the various results attained:
 Agarose gel electrophoresis image of digested K112808 |
 Failure 1 |
 The pBAD Insert |
 The Failed Ligation Attempt |
