June 8th- 17th

What are we trying to do?

We want the bacteria to lyse, to kill itself from the inside.

Well then, to do that we had to get a lysis gene in it. The thing is, with the lysis gene needs to be turned on by this specific substance called a promoter

There are two kinds of promoters, ones that make the gene they're attached to , be 'on' all the time. Then, you have ones that turn on when you want them to, in our case, when you add a mystery enzyme.

Our Step-wise Process:

How we tried to do this:

[http://hackteria.org/wiki/index.php/Step_1_-_Taking_dry_DNA_from_wells Step 1 - Taking dry DNA from wells]

[http://hackteria.org/wiki/index.php/Step_2_-_Transforming_competent_cells Step 2 - Transforming competent cells]

Step 3 - Picking a single colony.

Step 4 - Inoculating broth with Ampicillin-R (an antibiotic) and letting it grow for 18 hours.

Step 5 - Using the resulting culture for [http://hackteria.org/wiki/index.php/Mini-prep. mini-prep.] - a process used to purify plasmids and yields clean, usable DNA.

Step 6 - [http://hackteria.org/wiki/index.php/Digesting_the_DNA Digesting the DNA]

Step 7 - [http://hackteria.org/wiki/index.php/Gel_Electrophoresis Gel Electrophoresis]

Step 8- [http://hackteria.org/wiki/index.php/Ligation Ligation]

Step 9- Transformation and Inoculation

June 9th

[http://hackteria.org/wiki/index.php/The_June_9th_Image_Gallery The June 9th Image Gallery]

Images of the various results attained:

Agarose gel electrophoresis image of digested K112808

Failure 1

The pBAD Insert

The Failed Ligation Attempt