Team:Virginia Commonwealth/Results

From 2009.igem.org

(Difference between revisions)
(Experimental Results)
(Summary)
 
(2 intermediate revisions not shown)
Line 3: Line 3:
*Designed new BioBrick part family (UP-elements) and demonstrated function.
*Designed new BioBrick part family (UP-elements) and demonstrated function.
*Quantitatively characterized UP-elements using flow cytometry and RT-PCR.
*Quantitatively characterized UP-elements using flow cytometry and RT-PCR.
-
*Proposed a minimal measurement standard for transcriptional parts.
+
*Proposed a minimal measurement standard for characterizing transcriptional parts.
 +
 
==Experimental Results==
==Experimental Results==
-
We are currently generating and processing our data.  Please find a sample of flow cytometry fluorescence data below.
+
We are currently generating and processing our data and are looking forward to sharing our characterization results at the 2009 Jamboree (via poster and oral presentation).  Please find a sample of flow cytometry fluorescence data below.
-
[[Image:VCU 2009 sample flow data.png|thumb|center|600px|Sample flow cytometry data - Run 001 (leftmost) is a control and runs 002 and 003 are constitutive promoters]]
+
[[Image:VCU 2009 sample flow data.png|thumb|center|600px|Sample flow cytometry data - Run 001 (leftmost, black line) is a control and runs 002 (green line) and 003 (blue line) are constitutive promoters]]
==Collaboration==
==Collaboration==
We have been in discussion with the University of Virginia iGEM team regarding experimental procedures and project execution.
We have been in discussion with the University of Virginia iGEM team regarding experimental procedures and project execution.
[[Image:VGEM VCU meetup.jpg|thumb|left|400px|We met up with the University of Virginia iGEM team for a practice presentation on Oct. 18]]
[[Image:VGEM VCU meetup.jpg|thumb|left|400px|We met up with the University of Virginia iGEM team for a practice presentation on Oct. 18]]

Latest revision as of 03:22, 22 October 2009

Summary

  • Designed new BioBrick part family (UP-elements) and demonstrated function.
  • Quantitatively characterized UP-elements using flow cytometry and RT-PCR.
  • Proposed a minimal measurement standard for characterizing transcriptional parts.

Experimental Results

We are currently generating and processing our data and are looking forward to sharing our characterization results at the 2009 Jamboree (via poster and oral presentation). Please find a sample of flow cytometry fluorescence data below.

Sample flow cytometry data - Run 001 (leftmost, black line) is a control and runs 002 (green line) and 003 (blue line) are constitutive promoters

Collaboration

We have been in discussion with the University of Virginia iGEM team regarding experimental procedures and project execution.

We met up with the University of Virginia iGEM team for a practice presentation on Oct. 18