Team:British Columbia

From 2009.igem.org

(Difference between revisions)
(→The Bacterial Traffic Light: A flexible, modular, and transparent system for multi-level assessment of variable inputs.')
(→E.coli Traffic Light: A flexible, modular, and transparent system for multi-level assessment of variable inputs.')
 
(15 intermediate revisions not shown)
Line 1: Line 1:
{{Template:UBCiGEM2009_menu_home}}
{{Template:UBCiGEM2009_menu_home}}
-
==The Bacterial Traffic Light: <br> A ''flexible'', ''modular'', and ''transparent'' system for multi-level assessment of variable inputs.'==
+
=<font color="#00CC66">E.coli</font> <font color="#FFCC66">Traffic</font> <font color="#FF0000">Light</font>: <br> A ''flexible'', ''modular'', and ''transparent'' system for multi-level assessment of variable inputs.'=
-
Biosensors have a diverse variety of real-world functions, ranging from measuring blood glucose levels in diabetes patients to assessing environmental contamination of trace toxins. The majority of these sensors are highly specific for a single input, and their outputs often require specialized equipment such as surface plasmon resonance chips. Our project aims to create a biosensor that recognizes a specific target and alters its output fluorescence from green, to yellow, to red as a function of concentration up to critical levels (hence, a biological "traffic light").
+
Biosensors have a diverse variety of real-world functions, ranging from measuring blood glucose levels in diabetes patients to assessing environmental contamination of trace toxins. The majority of these sensors are highly specific for a single input, and their outputs often require specialized equipment such as surface plasmon resonance chips.  
 +
 
 +
Our project aims to create a biosensor that recognizes a specific target and alters its output fluorescence from green, to yellow, to red as a function of concentration up to critical levels (hence, a biological "traffic light").
Click the colours of the traffic light to learn about its different subparts!
Click the colours of the traffic light to learn about its different subparts!
<!-- [[Image:BritishColumbia-Trafficlight.png|center|400px]] -->
<!-- [[Image:BritishColumbia-Trafficlight.png|center|400px]] -->
 +
<html>
<html>
 +
 +
 +
<img src="/wiki/images/thumb/f/fc/E_coli_Traffic_Light_General.png/950px-E_coli_Traffic_Light_General.png" width=950 usemap="#trafficlight">
 +
<map name="trafficlight">
<map name="trafficlight">
<area shape="circle"
<area shape="circle"
Line 28: Line 35:
       href="/Team:British_Columbia/Jammer"
       href="/Team:British_Columbia/Jammer"
/>
/>
 +
</map>
</map>
</html>
</html>
-
 
-
<img src="/wiki/images/thumb/f/fc/E_coli_Traffic_Light_General.png/950px-E_coli_Traffic_Light_General.png" width=950 usemap="#trafficlight">
 
-
 
-
[[Image:E_coli_Traffic_Light_Subprojects.png|center|thumb||400px|The ''E. coli'' Traffic Light Biosensor is composed of three major subparts: variable arabinose-inducible promoters, RNA lock and key system, and reverse antisense promoters for input detection, color activation and traffic light switching respectively.]]
 
-
 
-
[[Image:E_coli_Traffic_Light_Step_by_Step.png|thumb|center|700px|Schematic black-box representation of the E. coli Biosensor that detects various concentration inputs and color outputs. The idea is discrete analog outputs based on a user-specified threshold for each range of concentration.]]
 
Line 43: Line 45:
[[Image:E_coli_Traffic_Light_General.png|thumb|center|950px|Schematic black-box representation of the E. coli Biosensor that detects various concentration inputs and color outputs. The idea is discrete analog outputs based on a user-specified threshold for each range of concentration.]]
[[Image:E_coli_Traffic_Light_General.png|thumb|center|950px|Schematic black-box representation of the E. coli Biosensor that detects various concentration inputs and color outputs. The idea is discrete analog outputs based on a user-specified threshold for each range of concentration.]]
-->
-->
-
 
-
The Traffic Light is composed of three distinct subparts:
 
-
* The [[Team:British_Columbia/pBAD|pBAD promoter family]]
 
-
* The [[Team:British_Columbia/LockandKey|lock and key riboregulation system]]
 
-
* The [[Team:British_Columbia/Jammer|Jammer]].
 

Latest revision as of 03:58, 22 October 2009

E.coli Traffic Light:
A flexible, modular, and transparent system for multi-level assessment of variable inputs.'

Biosensors have a diverse variety of real-world functions, ranging from measuring blood glucose levels in diabetes patients to assessing environmental contamination of trace toxins. The majority of these sensors are highly specific for a single input, and their outputs often require specialized equipment such as surface plasmon resonance chips.

Our project aims to create a biosensor that recognizes a specific target and alters its output fluorescence from green, to yellow, to red as a function of concentration up to critical levels (hence, a biological "traffic light").


Click the colours of the traffic light to learn about its different subparts!

Sensors: This links to the pBAD promoters that sense the arabinose. Lock and Key: this controls when the colors are produced The Jammer: this mRNA sequence blocks the key from opening the lock.



Retrieved from "http://2009.igem.org/Team:British_Columbia"