Team:Calgary/29 September 2009
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- | + | Colony PCR of rbs-aiiA-B0015 | |
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+ | This image illustrates the results of colony PCR of rbs-aiiA-B0015 | ||
+ | The initial denaturation process was set to 94oC for 6 mins, followed by 30 seconds of annealing time at 94oC and 45 seconds extension time for 55oC. | ||
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[[Image:2009igem.calgary.colonypcr.png|700px]] | [[Image:2009igem.calgary.colonypcr.png|700px]] | ||
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+ | For this PCR, BBK-CP-R and B0034-F primers were used. Wells 1,2,3,4,5 and 6 represent colonies 1,2,3,4,5,and negative control. The expected size of the construct is ~1.1kb | ||
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Latest revision as of 03:23, 22 October 2009
UNIVERSITY OF CALGARY