LB Broth

From 2009.igem.org

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[https://2009.igem.org/Team:Illinois/Protocols#Recipes Back to Protocols page]
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==LB Growth Media==
==LB Growth Media==
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**1L dH<sub>2</sub>0
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**10g NaCl
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*1L dH<sub>2</sub>0
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**5g yeast extract
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*10g NaCl
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**10g Bacto-tryptone
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*5g yeast extract
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*10g Bacto-tryptone
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If you are planning on adding an antibiotic add them after the media has cooled to around 50 degrees or less. Label each container of media. Example: "LB+AMP 100ug/mL 6/10/09".
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Latest revision as of 01:46, 19 July 2009

Click to go to the Illinois home page




Back to Protocols page

LB Growth Media

  • 1L dH20
  • 10g NaCl
  • 5g yeast extract
  • 10g Bacto-tryptone

If you are planning on adding an antibiotic add them after the media has cooled to around 50 degrees or less. Label each container of media. Example: "LB+AMP 100ug/mL 6/10/09".

Questions about our Wiki page? Please email us at illinoisiGEM@gmail.com.