Team:KULeuven/Used Parts

From 2009.igem.org

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=Individual parts=
=Individual parts=
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!| Partnumber||Short description|| Resistance || Status||Location||Additional notes
!| Partnumber||Short description|| Resistance || Status||Location||Additional notes
|- align="center"
|- align="center"
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| <partinfo>BBa_J23066 icon</partinfo> ||Ribokey|| Ap ||Available here|| -80, F1-72 ||   
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| {{kulpart|BBa_J23066|icon}} ||Ribokey|| A/A ||Ordered at GeneArt|| ||   
|- align="center"
|- align="center"
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| <partinfo>BBa_K238005 icon</partinfo>|| antikey || ||  Biobrick made || ||  
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| {{kulpart|BBa_K238005|icon}}|| antikey || A/A ||  Ordered at GeneArt/ Received || ||  
|-align="center"
|-align="center"
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| <partinfo>BBa_J23078 icon</partinfo>|| lock || Ap || Available here || -80, F1-41 ||  
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| {{kulpart|BBa_J23078|icon}}|| lock || Ap || Ordered at GeneArt/ Received || ||  
|-align="center"
|-align="center"
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| <partinfo>BBa_B0015 icon </partinfo> ||Terminator|| || in registry||  ||  
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| {{kulpart|BBa_B0015|icon}} ||Terminator|| Ak || Available here|| -80 || Can be found in -20 freezer
|-align="center"
|-align="center"
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| <partinfo>BBa_I742142 icon </partinfo>|| Sam8 || || ordered || || not in distribution, only in Edinburgh 2007 submissions 
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| {{kulpart|BBa_B0032|icon}}|| RBS || Ap || Available here || -80 ||  
|-align="center"
|-align="center"
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| <partinfo>BBa_I742141 icon </partinfo>|| Sam5 || || ordered || || not in distribution, only in Edinburgh 2007 submissions 
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| {{kulpart|BBa_J23110|icon}}|| promotor || Ap || Available here || -80 || Can be found in -20 freezer
|-align="center"
|-align="center"
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| <partinfo>BBa_I742107 icon</partinfo>|| comt || || in registry || in registry ||  
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| {{kulpart|BBa_I742106|icon}}|| RBS + Sam8 || A || Received from Edinburgh || -20 ||  
|-align="center"
|-align="center"
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| || fcs || || mail sent || || gene from [[http://www.ncbi.nlm.nih.gov/pubmed/14602615 1]], [[http://www.springerlink.com/content/bj47xnqn5kr65wvp/fulltext.pdf 2]] and [[http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=17437627#id538997 3]]
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| {{kulpart|BBa_K238007|icon}}|| Sam5 || A || Received from Edinburgh || -20 ||
|-align="center"
|-align="center"
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| || ech || || mail sent || || gene from [[http://www.ncbi.nlm.nih.gov/pubmed/14602615 1]], [[http://www.springerlink.com/content/bj47xnqn5kr65wvp/fulltext.pdf 2]] and [[http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=17437627#id538997 3]]
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| {{kulpart|BBa_I742109|icon}}|| comt || A/A || Received from Edinburgh/available in iGEM plates || -20 ||  
|-align="center"
|-align="center"
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| <partinfo>BBa_B0032 icon</partinfo>|| RBS || || || ||  
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| {{kulpart|BBa_I742115|icon}}|| fcs || Ap || Received from Edinburgh || -20 ||
|-align="center"
|-align="center"
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| <partinfo>BBa_J23110 icon</partinfo>|| promotor || || || ||  
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| {{kulpart|BBa_I742113|icon}}|| ech || Ap || Received from Edinburgh || -20 ||  
|-align="center"
|-align="center"
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| <partinfo>BBa_J58105 icon</partinfo>|| Vanillin Binding protein || || mailed Valencia Univ. || || sequence inconsistent on partsregistry
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| {{kulpart|BBa_K238008|icon}}|| VirA receptor || || Primers ordered || || This is purified from Agrobacterium tumefaciens
|-align="center"
|-align="center"
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| <partinfo>BBa_J58104 icon</partinfo>|| Trg envZ fusion protein || || mailed Valencia Univ. || || sequence inconsistent on partsregistry
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| {{kulpart|BBa_K238009|icon}}|| VirG activator || || Primers ordered || || This is purified from Agrobacterium tumefaciens
|-align="center"
|-align="center"
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| <partinfo>BBa_R0084 icon</partinfo>|| ompF promotor || Primers chosen || || || from [[http://www.ncbi.nlm.nih.gov/pubmed/19240136 1]]
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| {{kulpart|BBa_K238010|icon}}|| RpoA  || || Primers ordered || || This is purified from Agrobacterium tumefaciens and is necessary for VirG to work in E.coli
|-align="center"
|-align="center"
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| {{kulpart|BBa_K238011|icon}}|| VirB promoter region  || || Primers ordered || || This is purified from Agrobacterium tumefaciens and has virboxes that bind VirG
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|-align="center"
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| {{kulpart|BBa_K238000|icon}}|| YcgF/E promotor || || Region purified from E.coli strain MC4100|| -20 || Blue light promotor region from [[http://www.ncbi.nlm.nih.gov/pubmed/19240136 1]]
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|-align="center"
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| {{kulpart|BBa_K238013|icon}}|| YcgF/E promotor || || In progress of being made|| || Exact blue light promotor from [[http://www.ncbi.nlm.nih.gov/pubmed/19240136 1]]
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|-align="center"
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| {{kulpart|pSB3K3|icon}}|| Plasmid backbone || || From distribution plates|| || Kanamycine resistant plasmid
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|-align="center"
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| {{kulpart|BBa_K145015|icon}}|| GFP reporter || || From last years glycerol stock|| ||GFP+LVA+terminator
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|-align="center"
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| {{kulpart|BBa_K145201|icon}}|| TetR generator || || From last years glycerol stock|| || Promotor+RBS+tetR+terminator
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|-align="center"
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| {{kulpart|BBa_E0240|icon}}|| GFP reporter || || From last years glycerol stock|| || RBS+GFP+terminator
|}
|}
=Composite parts=
=Composite parts=
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[[:Category:Team:KULeuven/BioBricks]]
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{| border ="1" cellpadding="5" width=”100%” align="center" style="background:#f5faff; border:#cedff2"
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!| Partnumber||Short description|| Resistance || Status||Location||Additional notes
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|-align="center"
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| {{kulpart|BBa_K238001|icon}}|| VirB promotor + key || || Biobrick in registry || ||
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|-align="center"
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| {{kulpart|BBa_K238002|icon}}|| Blr promotor + key || || Biobrick in registry || ||
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|-align="center"
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| {{kulpart|BBa_K238003|icon}}|| Vanillin receptor || || Biobrick in registry || ||
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|-align="center"
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| {{kulpart|BBa_K238004|icon}}|| Vanillin synthesis|| || Biobrick in registry || || 5 paths in pathway never tested together in E. coli
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|-align="center"
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| {{kulpart|BBa_K238006|icon}}|| Short Vanillin synthesis|| || Biobrick in registry || || Short version containing the last 2 genes of the pathway, these have been tested in E. coli
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|-align="center"
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| {{kulpart|BBa_K238012|icon}}|| Short Vanillin synthesis II|| || Biobrick in registry || || Short version containing the first 3 parts of the pathway, these have not been tested in E. coli
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|}
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[[Category:Team:KULeuven/BioBricks]]
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{{Team:KULeuven/Common2/PageFooter}}

Latest revision as of 11:56, 21 September 2009

Individual parts

PartnumberShort description Resistance StatusLocationAdditional notes
Ribokey A/A Ordered at GeneArt
antikey A/A Ordered at GeneArt/ Received
lock Ap Ordered at GeneArt/ Received
Terminator Ak Available here -80 Can be found in -20 freezer
RBS Ap Available here -80
promotor Ap Available here -80 Can be found in -20 freezer
RBS + Sam8 A Received from Edinburgh -20
Sam5 A Received from Edinburgh -20
comt A/A Received from Edinburgh/available in iGEM plates -20
fcs Ap Received from Edinburgh -20
ech Ap Received from Edinburgh -20
VirA receptor Primers ordered This is purified from Agrobacterium tumefaciens
VirG activator Primers ordered This is purified from Agrobacterium tumefaciens
RpoA Primers ordered This is purified from Agrobacterium tumefaciens and is necessary for VirG to work in E.coli
VirB promoter region Primers ordered This is purified from Agrobacterium tumefaciens and has virboxes that bind VirG
YcgF/E promotor Region purified from E.coli strain MC4100 -20 Blue light promotor region from http://www.ncbi.nlm.nih.gov/pubmed/19240136 1
YcgF/E promotor In progress of being made Exact blue light promotor from http://www.ncbi.nlm.nih.gov/pubmed/19240136 1
Plasmid backbone From distribution plates Kanamycine resistant plasmid
GFP reporter From last years glycerol stock GFP+LVA+terminator
TetR generator From last years glycerol stock Promotor+RBS+tetR+terminator
GFP reporter From last years glycerol stock RBS+GFP+terminator

Composite parts

PartnumberShort description Resistance StatusLocationAdditional notes
VirB promotor + key Biobrick in registry
Blr promotor + key Biobrick in registry
Vanillin receptor Biobrick in registry
Vanillin synthesis Biobrick in registry 5 paths in pathway never tested together in E. coli
Short Vanillin synthesis Biobrick in registry Short version containing the last 2 genes of the pathway, these have been tested in E. coli
Short Vanillin synthesis II Biobrick in registry Short version containing the first 3 parts of the pathway, these have not been tested in E. coli

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