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| ===Results=== | | ===Results=== |
| ''Maria and Afton'' | | ''Maria and Afton'' |
- | * (+) Control: NEB 10 beta on LB
| |
- | **lawn growth
| |
- | * (+) Control: NEB 10 beta on LB shocked
| |
- | **lawn growth
| |
- | * (-) Control: NEB 10 beta on Cm
| |
- | **no growth
| |
- | * NEB 10 beta with BioBrick part (J23106, J06702, pSB4C5)
| |
- | **Approximately 30 small colonies
| |
| * All of the promoters showed up on the gel electrophoresis in a mostly straight line. | | * All of the promoters showed up on the gel electrophoresis in a mostly straight line. |
| + | * Transformation Results |
| + | {| cellpadding="5" border="1" padding="5" |
| + | |+ |
| + | |- |
| + | ! Name !! Plate !! Growth Observation |
| + | |- |
| + | | Control (+) NEB10β || LB || lawn |
| + | |- |
| + | | Control (+) NEB10β shocked || LB || lawn |
| + | |- |
| + | | Control (-) NEB10β || Cm || no growth |
| + | |- |
| + | | J23106, J06702, pSB4C5 ligation || Cm || ~30 small colonies |
| + | |} |
| + | |
| + | * Miniprep Results: |
| + | {| cellpadding="5" border="1" padding="5" |
| + | |+ |
| + | |- |
| + | ! Part No.!! A260 (nm) !! A280 (nm) !! A260/A280 !! Vol. (µL) !! Conc. (µg/mL) !! Amt (µg) |
| + | |- |
| + | | J23101 || 0.050 || 0.039 || 1.20 || 47 || 75 || 4.70 |
| + | |- |
| + | | J23102 || 0.030 || 0.025 || 1.20 || 58 || 45 || 3.48 |
| + | |- |
| + | | J23103 || 0.044 || 0.032 || 1.38 || 56 || 66 || 4.93 |
| + | |- |
| + | | J23104 || 0.033 || 0.028 || 1.18 || 54 || 49 || 3.56 |
| + | |- |
| + | | J23105 || 0.037 || 0.029 || 1.28 || 32 || 56 || 1.78 |
| + | |- |
| + | | J23107 || 0.054 || 0.040 || 1.35 || 47 || 81 || 3.81 |
| + | |} |
| [[User:Trentay|Trentay]] 13:44, 14 July 2009 (UTC) | | [[User:Trentay|Trentay]] 13:44, 14 July 2009 (UTC) |
| + | |
| + | * Gel Electrophoresis Results |
| + | [[Image:2009-07-14 digest06 100-105,107.jpg|350px|thumb|none|from left to right: ladder, 100-105,107]] |
| | | |
| ''Kevin and Adam'' | | ''Kevin and Adam'' |
| *Miniprep results: | | *Miniprep results: |
| + | {| cellpadding="5" border="1" padding="5" |
| + | |+ |
| + | |- |
| + | ! Part No.!! A260 (nm) !! A280 (nm) !! A260/A280 !! Vol. (µL) !! Conc. (µg/mL) !! Amt (µg) |
| + | |- |
| + | |E0240||.033||.019||1.737||124||66||8.184 |
| + | |- |
| + | |pSB4C5||.054||.036||1.5||123.5||108||13.34 |
| + | |} |
| [[User:Bussingkm|Bussingkm]] 15:21, 14 July 2009 (UTC) | | [[User:Bussingkm|Bussingkm]] 15:21, 14 July 2009 (UTC) |
| | | |
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| | | |
| ''Kevin and Adam'' | | ''Kevin and Adam'' |
| + | *perform complete digestion and ligation |
| *construct biobrick device (pSB4C5, BBa_E0240, BBa_J23100), and (pSB4C5, BBa_E0240, BBa_J23106) | | *construct biobrick device (pSB4C5, BBa_E0240, BBa_J23100), and (pSB4C5, BBa_E0240, BBa_J23106) |
| [[User:Bussingkm|Bussingkm]] 15:20, 14 July 2009 (UTC) | | [[User:Bussingkm|Bussingkm]] 15:20, 14 July 2009 (UTC) |
May
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| [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/2_May_2009&action=edit 2]
| [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/3_May_2009&action=edit 3]
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[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/4_May_2009&action=edit 4]
| [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/5_May_2009&action=edit 5]
| [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/6_May_2009&action=edit 6]
| [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/7_May_2009&action=edit 7]
| [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/8_May_2009&action=edit 8]
| [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/9_May_2009&action=edit 9]
| [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/10_May_2009&action=edit 10]
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[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/11_May_2009&action=edit 11]
| [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/12_May_2009&action=edit 12]
| [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/13_May_2009&action=edit 13]
| [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/14_May_2009&action=edit 14]
| [http://2009.igem.org/Virginia_Commonwealth/15_May_2009 15]
| [http://2009.igem.org/Virginia_Commonwealth/16_May_2009 16]
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[http://2009.igem.org/Virginia_Commonwealth/18_May_2009 18]
| [http://2009.igem.org/Virginia_Commonwealth/19_May_2009 19]
| [http://2009.igem.org/Virginia_Commonwealth/20_May_2009 20]
| [http://2009.igem.org/Virginia_Commonwealth/21_May_2009 21]
| [http://2009.igem.org/Virginia_Commonwealth/22_May_2009 22]
| [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/23_May_2009&action=edit 23]
| [http://2009.igem.org/Virginia_Commonwealth/24_May_2009 24]
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[http://2009.igem.org/Virginia_Commonwealth/25_May_2009 25]
| [http://2009.igem.org/Virginia_Commonwealth/26_May_2009 26]
| [http://2009.igem.org/Virginia_Commonwealth/27_May_2009 27]
| [http://2009.igem.org/Virginia_Commonwealth/28_May_2009 28]
| [http://2009.igem.org/Virginia_Commonwealth/29_May_2009 29]
| [http://2009.igem.org/Virginia_Commonwealth/30_May_2009 30]
| [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/31_May_2009&action=edit 31]
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June
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M | T | W | T | F | S | S
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[http://2009.igem.org/Virginia_Commonwealth/1_June_2009 1]
| [http://2009.igem.org/Virginia_Commonwealth/2_June_2009 2]
| [http://2009.igem.org/Virginia_Commonwealth/3_June_2009 3]
| [http://2009.igem.org/Virginia_Commonwealth/4_June_2009 4]
| [http://2009.igem.org/Virginia_Commonwealth/5_June_2009 5]
| [http://2009.igem.org/Virginia_Commonwealth/6_June_2009 6]
| [http://2009.igem.org/Virginia_Commonwealth/7_June_2009 7]
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[http://2009.igem.org/Virginia_Commonwealth/8_June_2009 8]
| [http://2009.igem.org/Virginia_Commonwealth/9_June_2009 9]
| [http://2009.igem.org/Virginia_Commonwealth/10_June_2009 10]
| [http://2009.igem.org/Virginia_Commonwealth/11_June_2009 11]
| [http://2009.igem.org/Virginia_Commonwealth/12_June_2009 12]
| [http://2009.igem.org/Virginia_Commonwealth/13_June_2009 13]
| [http://2009.igem.org/Virginia_Commonwealth/14_June_2009 14]
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[http://2009.igem.org/Virginia_Commonwealth/15_June_2009 15]
| [http://2009.igem.org/Virginia_Commonwealth/16_June_2009 16]
| [http://2009.igem.org/Virginia_Commonwealth/17_June_2009 17]
| [http://2009.igem.org/Virginia_Commonwealth/18_June_2009 18]
| [http://2009.igem.org/Virginia_Commonwealth/19_June_2009 19]
| [http://2009.igem.org/Virginia_Commonwealth/20_June_2009 20]
| [http://2009.igem.org/Virginia_Commonwealth/21_June_2009 21]
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[http://2009.igem.org/Virginia_Commonwealth/22_June_2009 22]
| [http://2009.igem.org/Virginia_Commonwealth/23_June_2009 23]
| [http://2009.igem.org/Virginia_Commonwealth/24_June_2009 24]
| [http://2009.igem.org/Virginia_Commonwealth/25_June_2009 25]
| [http://2009.igem.org/Virginia_Commonwealth/26_June_2009 26]
| [http://2009.igem.org/Virginia_Commonwealth/27_June_2009 27]
| [http://2009.igem.org/Virginia_Commonwealth/28_June_2009 28]
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[http://2009.igem.org/Virginia_Commonwealth/29_June_2009 29]
| [http://2009.igem.org/Virginia_Commonwealth/30_June_2009 30]
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July
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M | T | W | T | F | S | S
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| [http://2009.igem.org/Virginia_Commonwealth/1_July_2009 1]
| [http://2009.igem.org/Virginia_Commonwealth/2_July_2009 2]
| [http://2009.igem.org/Virginia_Commonwealth/3_July_2009 3]
| [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/4_July_2009&action=edit 4]
| [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/5_July_2009&action=edit 5]
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[http://2009.igem.org/Virginia_Commonwealth/6_July_2009 6]
| [http://2009.igem.org/Virginia_Commonwealth/7_July_2009 7]
| [http://2009.igem.org/Virginia_Commonwealth/8_July_2009 8]
| [http://2009.igem.org/Virginia_Commonwealth/9_July_2009 9]
| [http://2009.igem.org/Virginia_Commonwealth/10_July_2009 10]
| [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/11_July_2009&action=edit 11]
| [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/12_July_2009&action=edit 12]
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[http://2009.igem.org/Virginia_Commonwealth/13_July_2009 13]
| [http://2009.igem.org/Virginia_Commonwealth/14_July_2009 14]
| [http://2009.igem.org/Virginia_Commonwealth/15_July_2009 15]
| [http://2009.igem.org/Virginia_Commonwealth/16_July_2009 16]
| [http://2009.igem.org/Virginia_Commonwealth/17_July_2009 17]
| [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/18_July_2009&action=edit 18]
| [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/19_July_2009&action=edit 19]
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[http://2009.igem.org/Virginia_Commonwealth/20_July_2009 20]
| [http://2009.igem.org/Virginia_Commonwealth/21_July_2009 21]
| [http://2009.igem.org/Virginia_Commonwealth/22_July_2009 22]
| [http://2009.igem.org/Virginia_Commonwealth/23_July_2009 23]
| [http://2009.igem.org/Virginia_Commonwealth/24_July_2009 24]
| [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/25_July_2009&action=edit 25]
| [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/26_July_2009&action=edit 26]
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[http://2009.igem.org/Virginia_Commonwealth/27_July_2009 27]
| [http://2009.igem.org/Virginia_Commonwealth/28_July_2009 28]
| [http://2009.igem.org/Virginia_Commonwealth/29_July_2009 29]
| [http://2009.igem.org/Virginia_Commonwealth/30_July_2009 30]
| [http://2009.igem.org/Virginia_Commonwealth/31_July_2009 31]
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August
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| [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/1_August_2009&action=edit 1]
| [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/2_August_2009&action=edit 2]
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[http://2009.igem.org/Virginia_Commonwealth/3_August_2009 3]
| [http://2009.igem.org/Virginia_Commonwealth/4_August_2009 4]
| [http://2009.igem.org/Virginia_Commonwealth/5_August_2009 5]
| [http://2009.igem.org/Virginia_Commonwealth/6_August_2009 6]
| [http://2009.igem.org/Virginia_Commonwealth/7_August_2009 7]
| [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/8_August_2009&action=edit 8]
| [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/9_August_2009&action=edit 9]
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[http://2009.igem.org/Virginia_Commonwealth/10_August_2009 10]
| [http://2009.igem.org/Virginia_Commonwealth/11_August_2009 11]
| [http://2009.igem.org/Virginia_Commonwealth/12_August_2009 12]
| [http://2009.igem.org/Virginia_Commonwealth/13_August_2009 13]
| [http://2009.igem.org/Virginia_Commonwealth/14_August_2009 14]
| [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/15_August_2009&action=edit 15]
| [http://2009.igem.org/Virginia_Commonwealth/16_August_2009 16]
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[http://2009.igem.org/Virginia_Commonwealth/17_August_2009 17]
| [http://2009.igem.org/Virginia_Commonwealth/18_August_2009 18]
| [http://2009.igem.org/Virginia_Commonwealth/19_August_2009 19]
| [http://2009.igem.org/Virginia_Commonwealth/20_August_2009 20]
| [http://2009.igem.org/Virginia_Commonwealth/21_August_2009 21]
| [http://2009.igem.org/Virginia_Commonwealth/22_August_2009 22]
| [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/23_August_2009&action=edit 23]
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[http://2009.igem.org/Virginia_Commonwealth/24_August_2009 24]
| [http://2009.igem.org/Virginia_Commonwealth/25_August_2009 25]
| [http://2009.igem.org/Virginia_Commonwealth/26_August_2009 26]
| [http://2009.igem.org/Virginia_Commonwealth/27_August_2009 27]
| [http://2009.igem.org/Virginia_Commonwealth/28_August_2009 28]
| [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/29_August_2009&action=edit 29]
| [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/30_August_2009&action=edit 30]
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[http://2009.igem.org/Virginia_Commonwealth/31_August_2009 31]
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September
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| [http://2009.igem.org/Virginia_Commonwealth/1_September_2009 1]
| [http://2009.igem.org/Virginia_Commonwealth/2_September_2009 2]
| [http://2009.igem.org/Virginia_Commonwealth/3_September_2009 3]
| [http://2009.igem.org/Virginia_Commonwealth/4_September_2009 4]
| [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/5_September_2009&action=edit 5]
| [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/6_September_2009&action=edit 6]
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[http://2009.igem.org/Virginia_Commonwealth/7_September_2009 7]
| [http://2009.igem.org/Virginia_Commonwealth/8_September_2009 8]
| [http://2009.igem.org/Virginia_Commonwealth/9_September_2009 9]
| [http://2009.igem.org/Virginia_Commonwealth/10_September_2009 10]
| [http://2009.igem.org/Virginia_Commonwealth/11_September_2009 11]
| [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/12_September_2009&action=edit 12]
| [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/13_September_2009&action=edit 13]
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[http://2009.igem.org/Virginia_Commonwealth/14_September_2009 14]
| [http://2009.igem.org/Virginia_Commonwealth/15_September_2009 15]
| [http://2009.igem.org/Virginia_Commonwealth/16_September_2009 16]
| [http://2009.igem.org/Virginia_Commonwealth/17_September_2009 17]
| [http://2009.igem.org/Virginia_Commonwealth/18_September_2009 18]
| [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/19_September_2009&action=edit 19]
| [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/20_September_2009&action=edit 20]
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[http://2009.igem.org/Virginia_Commonwealth/21_September_2009 21]
| [http://2009.igem.org/Virginia_Commonwealth/22_September_2009 22]
| [http://2009.igem.org/Virginia_Commonwealth/23_September_2009 23]
| [http://2009.igem.org/Virginia_Commonwealth/24_September_2009 24]
| [http://2009.igem.org/Virginia_Commonwealth/25_September_2009 25]
| [http://2009.igem.org/Virginia_Commonwealth/26_September_2009 26]
| [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/27_September_2009&action=edit 27]
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[http://2009.igem.org/Virginia_Commonwealth/28_September_2009 28]
| [http://2009.igem.org/Virginia_Commonwealth/29_September_2009 29]
| [http://2009.igem.org/Virginia_Commonwealth/30_September_2009 30]
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October
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| [http://2009.igem.org/Virginia_Commonwealth/1_October_2009 1]
| [http://2009.igem.org/Virginia_Commonwealth/2_October_2009 2]
| [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/3_October_2009&action=edit 3]
| [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/4_October_2009&action=edit 4]
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[http://2009.igem.org/Virginia_Commonwealth/5_October_2009 5]
| [http://2009.igem.org/Virginia_Commonwealth/6_October_2009 6]
| [http://2009.igem.org/Virginia_Commonwealth/7_October_2009 7]
| [http://2009.igem.org/Virginia_Commonwealth/8_October_2009 8]
| [http://2009.igem.org/Virginia_Commonwealth/9_October_2009 9]
| [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/10_October_2009&action=edit 10]
| [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/11_October_2009&action=edit 11]
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[http://2009.igem.org/Virginia_Commonwealth/12_October_2009 12]
| [http://2009.igem.org/Virginia_Commonwealth/13_October_2009 13]
| [http://2009.igem.org/Virginia_Commonwealth/14_October_2009 14]
| [http://2009.igem.org/Virginia_Commonwealth/15_October_2009 15]
| [http://2009.igem.org/Virginia_Commonwealth/16_October_2009 16]
| [http://2009.igem.org/Virginia_Commonwealth/17_October_2009 17]
| [http://2009.igem.org/Virginia_Commonwealth/18_October_2009 18]
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[http://2009.igem.org/Virginia_Commonwealth/19_October_2009 19]
| [http://2009.igem.org/Virginia_Commonwealth/20_October_2009 20]
| [http://2009.igem.org/Virginia_Commonwealth/21_October_2009 21]
| [http://2009.igem.org/Virginia_Commonwealth/22_October_2009 22]
| [http://2009.igem.org/Virginia_Commonwealth/23_October_2009 23]
| [http://2009.igem.org/Virginia_Commonwealth/24_October_2009 24]
| [http://2009.igem.org/Virginia_Commonwealth/25_October_2009 25]
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[http://2009.igem.org/Virginia_Commonwealth/26_October_2009 26]
| [http://2009.igem.org/Virginia_Commonwealth/27_October_2009 27]
| [http://2009.igem.org/Virginia_Commonwealth/28_October_2009 28]
| [http://2009.igem.org/Virginia_Commonwealth/29_October_2009 29]
| [http://2009.igem.org/Virginia_Commonwealth/30_October_2009 30]
| [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/31_October_2009&action=edit 31]
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Tuesday 14 July 2009
Results
Maria and Afton
- All of the promoters showed up on the gel electrophoresis in a mostly straight line.
- Transformation Results
Name | Plate | Growth Observation
|
Control (+) NEB10β | LB | lawn
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Control (+) NEB10β shocked | LB | lawn
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Control (-) NEB10β | Cm | no growth
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J23106, J06702, pSB4C5 ligation | Cm | ~30 small colonies
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Part No. | A260 (nm) | A280 (nm) | A260/A280 | Vol. (µL) | Conc. (µg/mL) | Amt (µg)
|
J23101 | 0.050 | 0.039 | 1.20 | 47 | 75 | 4.70
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J23102 | 0.030 | 0.025 | 1.20 | 58 | 45 | 3.48
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J23103 | 0.044 | 0.032 | 1.38 | 56 | 66 | 4.93
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J23104 | 0.033 | 0.028 | 1.18 | 54 | 49 | 3.56
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J23105 | 0.037 | 0.029 | 1.28 | 32 | 56 | 1.78
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J23107 | 0.054 | 0.040 | 1.35 | 47 | 81 | 3.81
|
Trentay 13:44, 14 July 2009 (UTC)
- Gel Electrophoresis Results
from left to right: ladder, 100-105,107
Kevin and Adam
Part No. | A260 (nm) | A280 (nm) | A260/A280 | Vol. (µL) | Conc. (µg/mL) | Amt (µg)
|
E0240 | .033 | .019 | 1.737 | 124 | 66 | 8.184
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pSB4C5 | .054 | .036 | 1.5 | 123.5 | 108 | 13.34
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Bussingkm 15:21, 14 July 2009 (UTC)
Craig and Clay
- The ampicillin plates left overnight again displayed no growth. The electrotransformed parts will be re-plated onto ampicillin-selective plates as well as non-selective plates.
Tasks
Maria and Afton
- Digest promoters: 101, 102, 103, 104
- Possible Ligation of promoters with parts J06702, pSB4C5
Trentay 15:13, 14 July 2009 (UTC)
Kevin and Adam
- perform complete digestion and ligation
- construct biobrick device (pSB4C5, BBa_E0240, BBa_J23100), and (pSB4C5, BBa_E0240, BBa_J23106)
Bussingkm 15:20, 14 July 2009 (UTC)
Craig and Clay
- The electrotransformed parts will be re-plated on ampicillin plates as well as on non-selective plates. Growth on non-selective plates will prove the existence of viable cells.
Wetlab
Maria and Afton
- Spectophotometry of promoters: 101, 102, 103, 104, 105, 107
- Digestion/Ligation of promoters
- Electrophoresis of promoter digestions: 101, 102, 103, 104, 105, 107, (100)
- Electrotransformation of ligated parts
- two Cm plate had an unidentified growth in the refrigerator.
- however, negative controls from the same plate stock show antibiotic is working
- Picked colonies for overnight culture of J23106 w/ J06702 reporter
- Made 3 overnight 5mL cultures of NEB 10 beta cell stock to prepare electrocompetent cells
Trentay 21:01, 14 July 2009 (UTC)
Kevin and Adam
- pSB4C5, BBa_E0240, and BBa_J06702 were frozen in box C-11 of the glycerol stocks
Bussingkm 15:22, 14 July 2009 (UTC)
Craig and Clay
- The electrotransformed parts were again plated on ampicillin-selective plates. They were also plated on non-selective media. One hundred microliters was used for each plate rather than the standard 35 microliters.
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