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Team:UNC Chapel Hill/26 July 2009
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*Miniprepped everything that was cultured overnight except for pSB1A3, which is not supposed to be red (WTF). Recultured pSB1A3 with a non-red colony. | *Miniprepped everything that was cultured overnight except for pSB1A3, which is not supposed to be red (WTF). Recultured pSB1A3 with a non-red colony. | ||
| + | *Made a positive control to test to see if the KAN plates were good. | ||
*Tested the Optical Density of the solutions. Spectrophotometer results: | *Tested the Optical Density of the solutions. Spectrophotometer results: | ||
**A1: -11.8 ng/uL | **A1: -11.8 ng/uL | ||
**A2: 3.5 ng/uL | **A2: 3.5 ng/uL | ||
| - | re-blanked here | + | ***re-blanked here |
**A3: -1.2 ng/uL | **A3: -1.2 ng/uL | ||
**A4: -7.1 ng/uL | **A4: -7.1 ng/uL | ||
**A5: -4.2 ng/uL | **A5: -4.2 ng/uL | ||
**A6: -6.9 ng/uL | **A6: -6.9 ng/uL | ||
| - | blanked with different water here | + | ***blanked with different water here |
**A7: -6.9 ng/uL | **A7: -6.9 ng/uL | ||
*Something is definitely wrong. Either with the miniprepping or the spectrophotometery. | *Something is definitely wrong. Either with the miniprepping or the spectrophotometery. | ||
Latest revision as of 19:54, 27 July 2009
- Miniprepped everything that was cultured overnight except for pSB1A3, which is not supposed to be red (WTF). Recultured pSB1A3 with a non-red colony.
- Made a positive control to test to see if the KAN plates were good.
- Tested the Optical Density of the solutions. Spectrophotometer results:
- A1: -11.8 ng/uL
- A2: 3.5 ng/uL
- re-blanked here
- A3: -1.2 ng/uL
- A4: -7.1 ng/uL
- A5: -4.2 ng/uL
- A6: -6.9 ng/uL
- blanked with different water here
- A7: -6.9 ng/uL
- Something is definitely wrong. Either with the miniprepping or the spectrophotometery.
