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Team:Osaka/Brainstorming2
From 2009.igem.org
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*orthogonal cell-cell signaling will be required for our ART painting | *orthogonal cell-cell signaling will be required for our ART painting | ||
Existing systems-> | Existing systems-> | ||
| - | *LuxI-LuxR | + | *LuxI-LuxR |
*LasI-LasR | *LasI-LasR | ||
*ciniI-cinR | *ciniI-cinR | ||
| Line 19: | Line 19: | ||
===patterning=== | ===patterning=== | ||
we have to control the motility of E.coli or salmonella on soft-agar plate | we have to control the motility of E.coli or salmonella on soft-agar plate | ||
| + | |||
===Stop rotation of flagella motor=== | ===Stop rotation of flagella motor=== | ||
| - | *use molecular clutch [[http://partsregistry.org/partsdb/get_part.cgi?part=BBa_K143032]] | + | *use molecular clutch (iGEM08 Imperial college) [[http://partsregistry.org/partsdb/get_part.cgi?part=BBa_K143032]] |
| + | ->However, this parts was characterized only in butilus. | ||
| + | |||
| + | ===Stop viased random walk=== | ||
| + | express che? | ||
| + | |||
| + | ===Stop flagellar assembly=== | ||
| + | *use the strain that lacks flagella gene. But Inactivating auto inducer will be needed! | ||
| + | |||
| + | |||
| + | ==Art Challange== | ||
| + | *no team had done this theme. Ethics and environmental issue was emphasized. | ||
Latest revision as of 20:11, 29 July 2009
Brainstorming with one brain that belongs to nori
Contents |
Operation
Cell to Cell communication (Quorum sensing)
- orthogonal cell-cell signaling will be required for our ART painting
Existing systems->
- LuxI-LuxR
- LasI-LasR
- ciniI-cinR
- RhII-RhIR
- Evaluate diffuse of signals - PoPs, RiPs and Degradation
Timer or counter(optional addon)
- well known genetic circuit
- light or sound induced?
Output -pattern and painting-
patterning
we have to control the motility of E.coli or salmonella on soft-agar plate
Stop rotation of flagella motor
- use molecular clutch (iGEM08 Imperial college) http://partsregistry.org/partsdb/get_part.cgi?part=BBa_K143032
->However, this parts was characterized only in butilus.
Stop viased random walk
express che?
Stop flagellar assembly
- use the strain that lacks flagella gene. But Inactivating auto inducer will be needed!
Art Challange
- no team had done this theme. Ethics and environmental issue was emphasized.
