Template:Team:KULeuven/30 July 2009/VanillinProduction

From 2009.igem.org

(Difference between revisions)
(New page: following parts were put in competent cells by electroporation: * ech; RBS + ech, in pSB1A2, {{kulpart|BBa_I742113}} * fcs; RBS + fcs, in pSB1A2, {{kulpart|BBa_I742115}} the cells were the...)
 
(3 intermediate revisions not shown)
Line 1: Line 1:
-
following parts were put in competent cells by electroporation:
+
Following parts were put into competent cells by electroporation:
-
* ech; RBS + ech, in pSB1A2, {{kulpart|BBa_I742113}}
+
*RBS + ''ech'', in {{kulpart|pSB1A2}}, {{kulpart|BBa_I742113}}
-
* fcs; RBS + fcs, in pSB1A2, {{kulpart|BBa_I742115}}
+
*RBS + ''fcs'', in {{kulpart|pSB1A2}}, {{kulpart|BBa_I742115}}
-
the cells were then put in liquid LB medium to recuperate from the procedure. after a few hours they were transfered on LB/amp plates and grown overnight at 37°C.
+
The cells were then transferred to liquid LBmedium to recuperate from the procedure. After a few hours they were transferred to LB/amp plates and grown overnight at 37°C.

Latest revision as of 09:43, 14 September 2009

Following parts were put into competent cells by electroporation:

  • RBS + ech, in ,
  • RBS + fcs, in ,

The cells were then transferred to liquid LBmedium to recuperate from the procedure. After a few hours they were transferred to LB/amp plates and grown overnight at 37°C.

Retrieved from "http://2009.igem.org/Template:Team:KULeuven/30_July_2009/VanillinProduction"