Template:Team:KULeuven/30 July 2009/VanillinProduction

From 2009.igem.org

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following parts were put in competent cells by electroporation:
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Following parts were put into competent cells by electroporation:
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*RBS + ech, in pSB1A2, {{kulpart|BBa_I742113}}
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*RBS + ''ech'', in {{kulpart|pSB1A2}}, {{kulpart|BBa_I742113}}
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*RBS + fcs, in pSB1A2, {{kulpart|BBa_I742115}}
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*RBS + ''fcs'', in {{kulpart|pSB1A2}}, {{kulpart|BBa_I742115}}
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the cells were then put in liquid LB medium to recuperate from the procedure. after a few hours they were transfered on LB/amp plates and grown overnight at 37°C.
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The cells were then transferred to liquid LBmedium to recuperate from the procedure. After a few hours they were transferred to LB/amp plates and grown overnight at 37°C.

Latest revision as of 09:43, 14 September 2009

Following parts were put into competent cells by electroporation:

  • RBS + ech, in ,
  • RBS + fcs, in ,

The cells were then transferred to liquid LBmedium to recuperate from the procedure. After a few hours they were transferred to LB/amp plates and grown overnight at 37°C.