"
August/8 August 2009
From 2009.igem.org
(Difference between revisions)
| (5 intermediate revisions not shown) | |||
| Line 5: | Line 5: | ||
(plate number)-(location on plate) (no. of colonies) | (plate number)-(location on plate) (no. of colonies) | ||
----------------------------------------------------------- | ----------------------------------------------------------- | ||
| - | 2-12H none | + | [http://partsregistry.org/wiki/index.php?title=Part:BBa_K081020 2-12H] none |
| - | 1-12C none | + | [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0071 1-12C] none |
| - | 1-12A none | + | [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0079 1-12A] none |
| - | 2-24G none | + | [http://partsregistry.org/wiki/index.php?title=Part:BBa_F1610 2-24G] none |
| - | 2-18F none | + | [http://partsregistry.org/wiki/index.php?title=Part:BBa_K118013 2-18F] none |
| - | 1-6O ~10 | + | [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0062 1-6O] ~10 |
| - | 1-10K ~10 | + | [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0077 1-10K] ~10 |
| - | 1-18L ~10 | + | [http://partsregistry.org/wiki/index.php?title=Part:BBa_I14017 1-18L] ~10 |
| Line 22: | Line 22: | ||
(sample No.) (concentration) [260/280] [260/230] | (sample No.) (concentration) [260/280] [260/230] | ||
--------------------------------------------------- | --------------------------------------------------- | ||
| - | 1-23L | + | [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0015 B0015](1-23L) 24.9 ng/uL 1.77 1.77 |
| - | 2-16F 36.0 ng/uL 1.77 1.99 | + | [http://partsregistry.org/wiki/index.php?title=Part:BBa_K118002 K118002](2-16F) 36.0 ng/uL 1.77 1.99 |
| - | 1-8O | + | [http://partsregistry.org/wiki/index.php?title=Part:BBa_I0462 I0462](1-8O) 19.3 ng/uL 1.65 1.59 |
| - | 1-2M | + | [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0034 B0034](1-2M) 18.4 ng/uL 1.64 2.01 |
| - | 1-19B 40.9 ng/uL 1.56 0.83 | + | [http://partsregistry.org/wiki/index.php?title=Part:BBa_I742158 I742158](1-19B) 40.9 ng/uL 1.56 0.83 |
| - | 2-16H 27.7 ng/uL 1.68 1.38 | + | [http://partsregistry.org/wiki/index.php?title=Part:BBa_K118003 K118003](2-16H) 27.7 ng/uL 1.68 1.38 |
| - | 1-14D | + | [http://partsregistry.org/wiki/index.php?title=Part:BBa_C0076 C0076](1-14D) 9.3 ng/uL 1.66 2.31 |
| - | 2-8M | + | [http://partsregistry.org/wiki/index.php?title=Part:BBa_C0179 C0179](2-8M) 9.9 ng/uL 1.65 1.90 |
| - | 2-16M | + | [http://partsregistry.org/wiki/index.php?title=Part:BBa_S03878 S03878](2-16M) 24.6 ng/uL 1.72 1.24 |
| - | 1-14F | + | [http://partsregistry.org/wiki/index.php?title=Part:BBa_C0077 C0077](1-14F) 2.5 ng/uL 1.11 0.25 failure? |
| - | 1-23J | + | [http://partsregistry.org/wiki/index.php?title=Part:BBa_I1466 I1466](1-23J) failure |
| Line 41: | Line 41: | ||
<tr> | <tr> | ||
<td width="257"> </td> | <td width="257"> </td> | ||
| - | <td>uL</td> | + | <td><B>uL</b></td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td> | + | <td>EcoRI</td> |
<td>1</td> | <td>1</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td> | + | <td>SpeI</td> |
<td>1</td> | <td>1</td> | ||
</tr><tr> | </tr><tr> | ||
| Line 58: | Line 58: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td> | + | <td>total</td> |
<td>20</td></tr> | <td>20</td></tr> | ||
</table> | </table> | ||
| - | ↓ | + | ↓ <br> |
| - | + | 37°C,22hr<br> | |
| - | ↓ | + | ↓ <br> |
| - | + | failure (SYBR Safe : 30min???)<br> | |
| + | <br> | ||
| + | |||
| + | [https://2009.igem.org/Team:Osaka/NOTES back to NOTES] | ||
Latest revision as of 07:37, 12 October 2009
Today we carried out the following procedures (in rough chronological order):
1. Checked the cell cultures transformed and plated out yesterday (8/7) for colony formation and made an approximate count of the number of colonies.
(plate number)-(location on plate) (no. of colonies)
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K081020 2-12H] none
[http://partsregistry.org/wiki/index.php?title=Part:BBa_R0071 1-12C] none
[http://partsregistry.org/wiki/index.php?title=Part:BBa_R0079 1-12A] none
[http://partsregistry.org/wiki/index.php?title=Part:BBa_F1610 2-24G] none
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K118013 2-18F] none
[http://partsregistry.org/wiki/index.php?title=Part:BBa_R0062 1-6O] ~10
[http://partsregistry.org/wiki/index.php?title=Part:BBa_R0077 1-10K] ~10
[http://partsregistry.org/wiki/index.php?title=Part:BBa_I14017 1-18L] ~10
2. Conducted a 'miniprep' to harvest and check concentration of plasmids from 10 sample. (incubated since yesterday 8/7~) Result of Nanodrop concentration check:
(sample No.) (concentration) [260/280] [260/230]
[http://partsregistry.org/wiki/index.php?title=Part:BBa_B0015 B0015](1-23L) 24.9 ng/uL 1.77 1.77
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K118002 K118002](2-16F) 36.0 ng/uL 1.77 1.99
[http://partsregistry.org/wiki/index.php?title=Part:BBa_I0462 I0462](1-8O) 19.3 ng/uL 1.65 1.59
[http://partsregistry.org/wiki/index.php?title=Part:BBa_B0034 B0034](1-2M) 18.4 ng/uL 1.64 2.01
[http://partsregistry.org/wiki/index.php?title=Part:BBa_I742158 I742158](1-19B) 40.9 ng/uL 1.56 0.83
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K118003 K118003](2-16H) 27.7 ng/uL 1.68 1.38
[http://partsregistry.org/wiki/index.php?title=Part:BBa_C0076 C0076](1-14D) 9.3 ng/uL 1.66 2.31
[http://partsregistry.org/wiki/index.php?title=Part:BBa_C0179 C0179](2-8M) 9.9 ng/uL 1.65 1.90
[http://partsregistry.org/wiki/index.php?title=Part:BBa_S03878 S03878](2-16M) 24.6 ng/uL 1.72 1.24
[http://partsregistry.org/wiki/index.php?title=Part:BBa_C0077 C0077](1-14F) 2.5 ng/uL 1.11 0.25 failure?
[http://partsregistry.org/wiki/index.php?title=Part:BBa_I1466 I1466](1-23J) failure
3. gel electrophoresis
| uL | |
| EcoRI | 1 |
| SpeI | 1 |
| 10*Buffer No.2 | 2 |
| DNA (EpsE) | 5 |
| total | 20 |
↓
37°C,22hr
↓
failure (SYBR Safe : 30min???)
