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Team:Virginia/12 August 2009
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*Ligated and transformed A09-12 (pSB3K3 + ArsR + E0240) | *Ligated and transformed A09-12 (pSB3K3 + ArsR + E0240) | ||
*New PCR to add HA to PCS. Not using a separated cycle instead running 29 cycles at 65C annealing temp | *New PCR to add HA to PCS. Not using a separated cycle instead running 29 cycles at 65C annealing temp | ||
| + | *DNA concentrations of gel extracts of fragments not good enough, hence: | ||
| + | *Amplifying more product for RFP, GFP and rRNA fragments | ||
Latest revision as of 20:19, 12 August 2009
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Overnight Results
- A09-11 had colonies and satellite colonies
Today
- Ligated and transformed A09-12 (pSB3K3 + ArsR + E0240)
- New PCR to add HA to PCS. Not using a separated cycle instead running 29 cycles at 65C annealing temp
- DNA concentrations of gel extracts of fragments not good enough, hence:
- Amplifying more product for RFP, GFP and rRNA fragments
