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- | {{Template:Valencia09iGEM}} | + | {{Template:Valencia09iGEM2}} |
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- | <div align="justify" style="position:relative; top:-5px; left:70px; width:500px">
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- | == '''Yeast Team''' ==
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- | <span style="color:black; align:justify; font-size:10pt; font-family: Verdana">
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- | An important part of our project is to work with '''yeasts''' in order to create each and everyone of the cell-pixels of our bioscreen. We will use some aequorin-transformed yeast strains.
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- | '''Aequorin''' is a luminiscent protein isolated from luminescent jellyfish and many other marine organisms that uses '''coelenterazine''' as its cofactor to produce light whenever Ca<SUP>2+</SUP> is bound to it. Using a '''chemical input''' (KOH, alkali shock) we will open '''Ca<SUP>2+</SUP> channels''' in the yeasts' membrane (Viladevall L, et al. J Biol Chem. (2004) 279 43614–43624), Ca<SUP>2+</SUP> will enter into the cells and we will get '''light''' as an '''output'''.
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- | Later we will try to reproduce the same response with an '''electrical stimulus''', using some hardware built by ourselves.
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- | [[Image:Wiki.JPG|400px|center]]
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- | </span>
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- | </div>
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- | <html>
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- | <head>
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- | <script type="text/javascript">
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- | function mouseOverCellTeam()
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- | {
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- | document.getElementById("b12").src="https://static.igem.org/mediawiki/2009/9/9a/CellTeam_off.gif"
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- | }
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- | function mouseOutCellTeam()
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- | {
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- | document.getElementById("b12").src="https://static.igem.org/mediawiki/2009/1/12/CellTeam_on.gif"
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- | }
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- | function mouseOverYeastTeam()
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- | {
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- | document.getElementById("b13").src="https://static.igem.org/mediawiki/2009/1/1d/YeastTeam_off.jpg"
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- | }
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- | function mouseOutYeastTeam()
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- | {
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- | document.getElementById("b13").src="https://static.igem.org/mediawiki/2009/a/a8/YeasTeam_on.jpg"
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- | }
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- | </script>
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- | </head>
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- | <body>
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- | <div style="position:relative; left:690px; top: -500px">
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- | <table border="0" cellpadding="0" cellspacing="0">
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- | <tr>
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- | <td><a href="https://2009.igem.org/Team:Valencia/CellTeam" target="_top" onmouseover="mouseOverCellTeam()"
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- | onmouseout="mouseOutCellTeam()">
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- | <img border="0" alt="Cell Team" src="https://static.igem.org/mediawiki/2009/1/12/CellTeam_on.gif" id="b12" /></a></td>
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- | </tr>
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- | <tr>
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- | <td><a href="https://2009.igem.org/Team:Valencia/YeastTeam" target="_top" onmouseover="mouseOverYeastTeam()"
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- | onmouseout="mouseOutYeastTeam()">
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- | <img border="0" alt="Yeas Team" src="https://static.igem.org/mediawiki/2009/a/a8/YeasTeam_on.jpg" id="b13" /></a></td>
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- | </tr>
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- | </table>
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- | </div>
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- | </body>
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- | </html>
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| + | <table cellpadding="5" cellspacing="5" overflow="hidden" align="center"> |
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| + | <html><a href="https://2009.igem.org/Team:Valencia/WetLab/YeastTeam" target="_top"> <img src="https://static.igem.org/mediawiki/2009/e/e8/Yeastteam.jpg" width="300" height="230"></a></html> |
| + | </td> |
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| + | <html></html> |
| + | </td> |
| + | </tr> |
| + | </table> |
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- | <br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br>
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- | <div style="position:absolute; top:380px; left:650px; overflow:hidden;"> | + | <!--<div style="position:absolute; top:420px; left:650px; overflow:hidden;"> |
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