Team:Calgary/6 July 2009

From 2009.igem.org

(Difference between revisions)
 
(8 intermediate revisions not shown)
Line 157: Line 157:
<br>
<br>
<div class="heading">
<div class="heading">
-
Descriptive Title of What You’re Doing
+
Transformation of <i>luxCDABE</i> into both pSB1AK3 and pSB1AC3
</div>
</div>
<br>
<br>
<div class="desc">
<div class="desc">
</html>
</html>
-
WIKI CODING HERE
+
* Ligated <i>luxCDABE</i> into both pSB1AK3 and pSB1AC3
 +
* Transformed ligated products into Top 10 cells and plated on AK plates for overnight incubation.
 +
 
<html>
<html>
</div>
</div>
Line 186: Line 188:
<br>
<br>
<div class="desc">
<div class="desc">
-
</html>Looked through many paper for estimates of initial values for each species in our model and estimates for the k values present in the model .  
+
</html>Looked through many paper for estimates of initial values for each species in our model and estimates for the k values present in the model.  
<br>cell volume : 0.58 -0.69 um^3
<br>cell volume : 0.58 -0.69 um^3
<br>Kubitschek HE. Cell volume increase in Escherichia coli after shifts to richer media. J Bacteriol. 1990 Jan172(1):94-101
<br>Kubitschek HE. Cell volume increase in Escherichia coli after shifts to richer media. J Bacteriol. 1990 Jan172(1):94-101
<br>RNA polymerase transcription rate :70nt/sec  
<br>RNA polymerase transcription rate :70nt/sec  
<br>Mutation per base pair per replication : Drake JW, A constant rate of spontaneous mutation in DNA-based microbes.Proc Natl Acad Sci U S A. 1991 Aug 1588(16):7160-4 Table 1
<br>Mutation per base pair per replication : Drake JW, A constant rate of spontaneous mutation in DNA-based microbes.Proc Natl Acad Sci U S A. 1991 Aug 1588(16):7160-4 Table 1
-
 
-
 
-
 
<html>
<html>
Line 221: Line 220:
<html/>
<html/>
-
Now that we have our gene of interest, LuxOD47E, Biobricked, we can start constuction to add the J13002 promoter and the B0015 terminator.  We'll do two constrcutions in parallel and see if one of them works.
+
Now that we have our gene of interest, LuxOD47E, Biobricked, we can start construction to add the J13002 promoter and the B0015 terminator.  We'll do two constructions in parallel and see if one of them works.
<Br>
<Br>
<Br>
<Br>
Line 227: Line 226:
<Br>
<Br>
<Br>
<Br>
-
* Performed Antarctic Phosphotase Treatment (NEB) on the recipients, followed by ligation of the inserts and recipients.
+
* Performed Antarctic Phosphatase Treatment (NEB) on the recipients, followed by ligation of the inserts and recipients.
<Br>
<Br>
<Br>
<Br>
Line 254: Line 253:
<br>
<br>
<div class="heading">
<div class="heading">
-
Descriptive Title of What You're Doing
+
Marketing and Ethics for July 6th 2009
</div>
</div>
<br>
<br>
<div class="desc">
<div class="desc">
</html>
</html>
-
WIKI CODING HERE
+
Today, I e-mailed out our sponsorship package to Shell Canada, Western Canada Oil Spill Cleanups, ConocoPhillips and Syncrude Inc. I will make follow-up calls to them next week. I also continued to work on the July newsletter.
-
 
+
<BR>
-
<html>
+
<BR>  
-
</div>
+
I also read some ethics papers regarding open sources VS closed sources.
-
</td>
+
-
</tr>
+
-
 
+
-
<tr>
+
-
<td height="10px" bgcolor="#222222">
+
-
</td>
+
-
</tr>
+
-
 
+
-
<tr>
+
-
<td>
+
-
<a name="Iman"></a>
+
-
<br>
+
-
<div class="heading">
+
-
IMAN
+
-
</div>
+
-
<br>
+
-
<div class="heading">
+
-
Descriptive Title of What You're Doing
+
-
</div>
+
-
<br>
+
-
<div class="desc">
+
-
</html>
+
-
WIKI CODING HERE
+
<html>
<html>
Line 306: Line 282:
<br>
<br>
<div class="heading">
<div class="heading">
-
Descriptive Title of What You're Doing
+
Sequencing Results Analysis!
</div>
</div>
<br>
<br>
<div class="desc">
<div class="desc">
</html>
</html>
-
WIKI CODING HERE
+
Sequencing results indicated successful construction of the luxOU construct. Glycerol stocks made.
-
 
+
-
<html>
+
-
</div>
+
-
</td>
+
-
</tr>
+
-
 
+
-
<tr>
+
-
<td height="10px" bgcolor="#222222">
+
-
</td>
+
-
</tr>
+
-
 
+
-
<tr>
+
-
<td>
+
-
<a name="Jeremy"></a>
+
-
<br>
+
-
<div class="heading">
+
-
JEREMY
+
-
</div>
+
-
<br>
+
-
<div class="heading">
+
-
Descriptive Title of What You're Doing
+
-
</div>
+
-
<br>
+
-
<div class="desc">
+
-
</html>
+
-
WIKI CODING HERE
+
-
 
+
-
<html>
+
-
</div>
+
-
</td>
+
-
</tr>
+
-
 
+
-
<tr>
+
-
<td height="10px" bgcolor="#222222">
+
-
</td>
+
-
</tr>
+
-
 
+
-
<tr>
+
-
<td>
+
-
<a name="Katie"></a>
+
-
<br>
+
-
<div class="heading">
+
-
KATIE
+
-
</div>
+
-
<br>
+
-
<div class="heading">
+
-
Descriptive Title of What You're Doing
+
-
</div>
+
-
<br>
+
-
<div class="desc">
+
-
</html>
+
-
WIKI CODING HERE
+
<html>
<html>
Line 399: Line 323:
<div class="desc">
<div class="desc">
</html>
</html>
-
Isolated Flurorescent proteins were constructed behind of R0040 or J13002, depending on whether or not the fluorescent protein contained RBS, and transformed into TOP10 cells.
+
Isolated Fluorescent proteins were constructed behind of R0040 or J13002, depending on whether or not the fluorescent protein contained RBS, and transformed into TOP10 cells.
<html>
<html>
Line 420: Line 344:
<br>
<br>
<div class="heading">
<div class="heading">
-
Descriptive Title of What You're Doing
+
Building Frenzy ala Second Life
</div>
</div>
<br>
<br>
<div class="desc">
<div class="desc">
</html>
</html>
-
WIKI CODING HERE
+
Some more building design for the labs, platforms, etc. was done today.
<html>
<html>
Line 446: Line 370:
<br>
<br>
<div class="heading">
<div class="heading">
-
Descriptive Title of What You're Doing
+
Half Way Point: Taking Stock
</div>
</div>
<br>
<br>
<div class="desc">
<div class="desc">
</html>
</html>
-
WIKI CODING HERE
+
Retrospective Notebook: This entry was not written on this day, but derived later from working notes I made that day.
 +
 
 +
The major systems I had built to date included:
 +
*DNA finding each other in polymer, communicating with RNAP
 +
*binding/unbinding of TetR with each other, and DNA, and control by the HUD
 +
*The HUD display system
 +
 
 +
The messages passed between these parts were beginning to crowd the chat channels I was using, I came up with a few rules of thumb to keep things sane. Each script state gets its own channel to listen on instead of using a single channel for everything, and wherever possible messages from one part to another use the same format: 'owner's ID','destination object's id','message label' as the first three parts of the message. Some messages don't need any more information than that, and some are broadcast messages that don't need a destination object id either. Others would have several additional pieces of information, each separated by a comma. This system has been very reliable, and it takes practically no effort to set up reliable new messages types now.
 +
 
 +
I believe I actually made a big diagram of all the messages to make sure that none were conflicting with each other, before realizing that if I just followed these rules there couldn't be any problems!
 +
 
 +
Also made a list of what I hadn't implemented yet:
 +
*Count mode, aka the Interaction Checker
 +
*Polymer maker, aka the Biobricker (later fully implemented! wohoo!)
 +
*DNA binding interactions, I had RNAP and repression working, but not translation/transcription or activation, and the system for termination was a little unstable too. (later fully rectified and implemented as well. yay!)
 +
 
 +
I also wrote a [http://igemcalgary.blogspot.com/2009/07/welcome-my-name-is-patrick-king-i-am.html blog post] today.
<html>
<html>
Line 480: Line 420:
I also wrote up some emails to  companies after researching the companies for the past few days. The rest of the marketing team edited the emails before I sent them off. I also call 8 other companies and 5 companies were interested in looking at our sponsorship package. I sent off the package and updated the iGEM marketing excel spreadsheet. I left a voicemail to the 3 companies and I'll follow up with them tomorrow or later this week. I got the contact information of the Marketing Lead for one of those 3 companies. My plan is to call the other 2 companies tomorrow to first: get a hold of someone in-charge of sponsorship and second: speak to them about our project, potential applications and how it may benefit their company.  
I also wrote up some emails to  companies after researching the companies for the past few days. The rest of the marketing team edited the emails before I sent them off. I also call 8 other companies and 5 companies were interested in looking at our sponsorship package. I sent off the package and updated the iGEM marketing excel spreadsheet. I left a voicemail to the 3 companies and I'll follow up with them tomorrow or later this week. I got the contact information of the Marketing Lead for one of those 3 companies. My plan is to call the other 2 companies tomorrow to first: get a hold of someone in-charge of sponsorship and second: speak to them about our project, potential applications and how it may benefit their company.  
 +
<html>
<html>
</div>
</div>
Line 504: Line 445:
<div class="desc">
<div class="desc">
</html>
</html>
-
I have gotten some books from the library on scripting. I'm learning by using bits ans pieces from other people's free scripts and modifying them to do different
+
I have gotten some books from the library on scripting. I'm learning by using bits ans pieces from other people's free scripts and modifying them to do different things. I have managed to make a script that is adjustable to make an object move around randomly.
<html>
<html>
Line 525: Line 466:
<br>
<br>
<div class="heading">
<div class="heading">
-
Descriptive Title of What You're Doing
+
Sequencing results from the J13002 + LuxO D47A + B0015 on pSB1AK3 that I prepared on Thursday
</div>
</div>
<br>
<br>
<div class="desc">
<div class="desc">
</html>
</html>
-
WIKI CODING HERE
+
The sequencing results were returned from the samples of J13002 + LuxO D47A + B0015 on pSB1AK3 that I sent down last day. These were confirmed positive. I have included the sequencing results for one of the samples and the alignments immediately thereafter. These results are the same for both of the samples that I tested, indicating that the colonies that I tested have the proper sequence for J13002 + LuxO D47A + B0015 on pSB1AK3.
 +
 
 +
<br><br>
 +
<center>
 +
[[Image:J13002LuxOD47AB0015sequence.jpg|700px]]
 +
</center>
 +
 
 +
Alignment results
 +
 
 +
<br><br>
 +
<center>
 +
[[Image:J13002LuxOD47AB0015comparison.jpg|700px]]
 +
 
 +
</center>
 +
 
<html>
<html>

Latest revision as of 21:21, 20 October 2009

University of Calgary

UNIVERSITY OF CALGARY



THIS MONTH

July
MTWTFSS
    [http://2009.igem.org/Team:Calgary/1_July_2009 1] [http://2009.igem.org/Team:Calgary/2_July_2009 2] [http://2009.igem.org/Team:Calgary/3_July_2009 3] [http://2009.igem.org/Team:Calgary/4_July_2009 4] [http://2009.igem.org/Team:Calgary/5_July_2009 5]
[http://2009.igem.org/Team:Calgary/6_July_2009 6] [http://2009.igem.org/Team:Calgary/7_July_2009 7] [http://2009.igem.org/Team:Calgary/8_July_2009 8] [http://2009.igem.org/Team:Calgary/9_July_2009 9] [http://2009.igem.org/Team:Calgary/10_July_2009 10] [http://2009.igem.org/Team:Calgary/11_July_2009 11] [http://2009.igem.org/Team:Calgary/12_July_2009 12]
[http://2009.igem.org/Team:Calgary/13_July_2009 13] [http://2009.igem.org/Team:Calgary/14_July_2009 14] [http://2009.igem.org/Team:Calgary/15_July_2009 15] [http://2009.igem.org/Team:Calgary/16_July_2009 16] [http://2009.igem.org/Team:Calgary/17_July_2009 17] [http://2009.igem.org/Team:Calgary/18_July_2009 18] [http://2009.igem.org/Team:Calgary/19_July_2009 19]
[http://2009.igem.org/Team:Calgary/20_July_2009 20] [http://2009.igem.org/Team:Calgary/21_July_2009 21] [http://2009.igem.org/Team:Calgary/22_July_2009 22] [http://2009.igem.org/Team:Calgary/23_July_2009 23] [http://2009.igem.org/Team:Calgary/24_July_2009 24] [http://2009.igem.org/Team:Calgary/25_July_2009 25] [http://2009.igem.org/Team:Calgary/26_July_2009 26]
[http://2009.igem.org/Team:Calgary/27_July_2009 27] [http://2009.igem.org/Team:Calgary/28_July_2009 28] [http://2009.igem.org/Team:Calgary/29_July_2009 29] [http://2009.igem.org/Team:Calgary/30_July_2009 30] [http://2009.igem.org/Team:Calgary/31_July_2009 31]


NOTEBOOK PAGE INDEX
Carol
Chinmoyee
Emily
Fahd
Iman
Jamie
Jeremy
Katie
Kevin
Mandy
Patrick
Prima
Stefan
Vicki



CALENDAR

May
MTWTFSS
        [http://2009.igem.org/Team:Calgary/1_May_2009 1] [http://2009.igem.org/Team:Calgary/2_May_2009 2] [http://2009.igem.org/Team:Calgary/3_May_2009 3]
[http://2009.igem.org/Team:Calgary/4_May_2009 4] [http://2009.igem.org/Team:Calgary/5_May_2009 5] [http://2009.igem.org/Team:Calgary/6_May_2009 6] [http://2009.igem.org/Team:Calgary/7_May_2009 7] [http://2009.igem.org/Team:Calgary/8_May_2009 8] [http://2009.igem.org/Team:Calgary/9_May_2009 9] [http://2009.igem.org/Team:Calgary/10_May_2009 10]
[http://2009.igem.org/Team:Calgary/11_May_2009 11] [http://2009.igem.org/Team:Calgary/12_May_2009 12] [http://2009.igem.org/Team:Calgary/13_May_2009 13] [http://2009.igem.org/Team:Calgary/14_May_2009 14] [http://2009.igem.org/Team:Calgary/15_May_2009 15] [http://2009.igem.org/Team:Calgary/16_May_2009 16] [http://2009.igem.org/Team:Calgary/17_May_2009 17]
[http://2009.igem.org/Team:Calgary/18_May_2009 18] [http://2009.igem.org/Team:Calgary/19_May_2009 19] [http://2009.igem.org/Team:Calgary/20_May_2009 20] [http://2009.igem.org/Team:Calgary/21_May_2009 21] [http://2009.igem.org/Team:Calgary/22_May_2009 22] [http://2009.igem.org/Team:Calgary/23_May_2009 23] [http://2009.igem.org/Team:Calgary/24_May_2009 24]
[http://2009.igem.org/Team:Calgary/25_May_2009 25] [http://2009.igem.org/Team:Calgary/26_May_2009 26] [http://2009.igem.org/Team:Calgary/27_May_2009 27] [http://2009.igem.org/Team:Calgary/28_May_2009 28] [http://2009.igem.org/Team:Calgary/29_May_2009 29] [http://2009.igem.org/Team:Calgary/30_May_2009 30] [http://2009.igem.org/Team:Calgary/31_May_2009 31]


June
MTWTFSS
[http://2009.igem.org/Team:Calgary/1_June_2009 1] [http://2009.igem.org/Team:Calgary/2_June_2009 2] [http://2009.igem.org/Team:Calgary/3_June_2009 3] [http://2009.igem.org/Team:Calgary/4_June_2009 4] [http://2009.igem.org/Team:Calgary/5_June_2009 5] [http://2009.igem.org/Team:Calgary/6_June_2009 6] [http://2009.igem.org/Team:Calgary/7_June_2009 7]
[http://2009.igem.org/Team:Calgary/8_June_2009 8] [http://2009.igem.org/Team:Calgary/9_June_2009 9] [http://2009.igem.org/Team:Calgary/10_June_2009 10] [http://2009.igem.org/Team:Calgary/11_June_2009 11] [http://2009.igem.org/Team:Calgary/12_June_2009 12] [http://2009.igem.org/Team:Calgary/13_June_2009 13] [http://2009.igem.org/Team:Calgary/14_June_2009 14]
[http://2009.igem.org/Team:Calgary/15_June_2009 15] [http://2009.igem.org/Team:Calgary/16_June_2009 16] [http://2009.igem.org/Team:Calgary/17_June_2009 17] [http://2009.igem.org/Team:Calgary/18_June_2009 18] [http://2009.igem.org/Team:Calgary/19_June_2009 19] [http://2009.igem.org/Team:Calgary/20_June_2009 20] [http://2009.igem.org/Team:Calgary/21_June_2009 21]
[http://2009.igem.org/Team:Calgary/22_June_2009 22] [http://2009.igem.org/Team:Calgary/23_June_2009 23] [http://2009.igem.org/Team:Calgary/24_June_2009 24] [http://2009.igem.org/Team:Calgary/25_June_2009 25] [http://2009.igem.org/Team:Calgary/26_June_2009 26] [http://2009.igem.org/Team:Calgary/27_June_2009 27] [http://2009.igem.org/Team:Calgary/28_June_2009 28]
[http://2009.igem.org/Team:Calgary/29_June_2009 29] [http://2009.igem.org/Team:Calgary/30_June_2009 30]


July
MTWTFSS
    [http://2009.igem.org/Team:Calgary/1_July_2009 1] [http://2009.igem.org/Team:Calgary/2_July_2009 2] [http://2009.igem.org/Team:Calgary/3_July_2009 3] [http://2009.igem.org/Team:Calgary/4_July_2009 4] [http://2009.igem.org/Team:Calgary/5_July_2009 5]
[http://2009.igem.org/Team:Calgary/6_July_2009 6] [http://2009.igem.org/Team:Calgary/7_July_2009 7] [http://2009.igem.org/Team:Calgary/8_July_2009 8] [http://2009.igem.org/Team:Calgary/9_July_2009 9] [http://2009.igem.org/Team:Calgary/10_July_2009 10] [http://2009.igem.org/Team:Calgary/11_July_2009 11] [http://2009.igem.org/Team:Calgary/12_July_2009 12]
[http://2009.igem.org/Team:Calgary/13_July_2009 13] [http://2009.igem.org/Team:Calgary/14_July_2009 14] [http://2009.igem.org/Team:Calgary/15_July_2009 15] [http://2009.igem.org/Team:Calgary/16_July_2009 16] [http://2009.igem.org/Team:Calgary/17_July_2009 17] [http://2009.igem.org/Team:Calgary/18_July_2009 18] [http://2009.igem.org/Team:Calgary/19_July_2009 19]
[http://2009.igem.org/Team:Calgary/20_July_2009 20] [http://2009.igem.org/Team:Calgary/21_July_2009 21] [http://2009.igem.org/Team:Calgary/22_July_2009 22] [http://2009.igem.org/Team:Calgary/23_July_2009 23] [http://2009.igem.org/Team:Calgary/24_July_2009 24] [http://2009.igem.org/Team:Calgary/25_July_2009 25] [http://2009.igem.org/Team:Calgary/26_July_2009 26]
[http://2009.igem.org/Team:Calgary/27_July_2009 27] [http://2009.igem.org/Team:Calgary/28_July_2009 28] [http://2009.igem.org/Team:Calgary/29_July_2009 29] [http://2009.igem.org/Team:Calgary/30_July_2009 30] [http://2009.igem.org/Team:Calgary/31_July_2009 31]


August
MTWTFSS
          [http://2009.igem.org/Team:Calgary/1_August_2009 1] [http://2009.igem.org/Team:Calgary/2_August_2009 2]
[http://2009.igem.org/Team:Calgary/3_August_2009 3] [http://2009.igem.org/Team:Calgary/4_August_2009 4] [http://2009.igem.org/Team:Calgary/5_August_2009 5] [http://2009.igem.org/Team:Calgary/6_August_2009 6] [http://2009.igem.org/Team:Calgary/7_August_2009 7] [http://2009.igem.org/Team:Calgary/8_August_2009 8] [http://2009.igem.org/Team:Calgary/9_August_2009 9]
[http://2009.igem.org/Team:Calgary/10_August_2009 10] [http://2009.igem.org/Team:Calgary/11_August_2009 11] [http://2009.igem.org/Team:Calgary/12_August_2009 12] [http://2009.igem.org/Team:Calgary/13_August_2009 13] [http://2009.igem.org/Team:Calgary/14_August_2009 14] [http://2009.igem.org/Team:Calgary/15_August_2009 15] [http://2009.igem.org/Team:Calgary/16_August_2009 16]
[http://2009.igem.org/Team:Calgary/17_August_2009 17] [http://2009.igem.org/Team:Calgary/18_August_2009 18] [http://2009.igem.org/Team:Calgary/19_August_2009 19] [http://2009.igem.org/Team:Calgary/20_August_2009 20] [http://2009.igem.org/Team:Calgary/21_August_2009 21] [http://2009.igem.org/Team:Calgary/22_August_2009 22] [http://2009.igem.org/Team:Calgary/23_August_2009 23]
[http://2009.igem.org/Team:Calgary/24_August_2009 24] [http://2009.igem.org/Team:Calgary/25_August_2009 25] [http://2009.igem.org/Team:Calgary/26_August_2009 26] [http://2009.igem.org/Team:Calgary/27_August_2009 27] [http://2009.igem.org/Team:Calgary/28_August_2009 28] [http://2009.igem.org/Team:Calgary/29_August_2009 29] [http://2009.igem.org/Team:Calgary/30_August_2009 30]
[http://2009.igem.org/Team:Calgary/31_August_2009 31]


September
MTWTFSS
  [http://2009.igem.org/Team:Calgary/1_September_2009 1] [http://2009.igem.org/Team:Calgary/2_September_2009 2] [http://2009.igem.org/Team:Calgary/3_September_2009 3] [http://2009.igem.org/Team:Calgary/4_September_2009 4] [http://2009.igem.org/Team:Calgary/5_September_2009 5] [http://2009.igem.org/Team:Calgary/6_September_2009 6]
[http://2009.igem.org/Team:Calgary/7_September_2009 7] [http://2009.igem.org/Team:Calgary/8_September_2009 8] [http://2009.igem.org/Team:Calgary/9_September_2009 9] [http://2009.igem.org/Team:Calgary/10_September_2009 10] [http://2009.igem.org/Team:Calgary/11_September_2009 11] [http://2009.igem.org/Team:Calgary/12_September_2009 12] [http://2009.igem.org/Team:Calgary/13_September_2009 13]
[http://2009.igem.org/Team:Calgary/14_September_2009 14] [http://2009.igem.org/Team:Calgary/15_September_2009 15] [http://2009.igem.org/Team:Calgary/16_September_2009 16] [http://2009.igem.org/Team:Calgary/17_September_2009 17] [http://2009.igem.org/Team:Calgary/18_September_2009 18] [http://2009.igem.org/Team:Calgary/19_September_2009 19] [http://2009.igem.org/Team:Calgary/20_September_2009 20]
[http://2009.igem.org/Team:Calgary/21_September_2009 21] [http://2009.igem.org/Team:Calgary/22_September_2009 22] [http://2009.igem.org/Team:Calgary/23_September_2009 23] [http://2009.igem.org/Team:Calgary/24_September_2009 24] [http://2009.igem.org/Team:Calgary/25_September_2009 25] [http://2009.igem.org/Team:Calgary/26_September_2009 26] [http://2009.igem.org/Team:Calgary/27_September_2009 27]
[http://2009.igem.org/Team:Calgary/28_September_2009 28] [http://2009.igem.org/Team:Calgary/29_September_2009 29] [http://2009.igem.org/Team:Calgary/30_September_2009 30]


October
MTWTFSS
      [http://2009.igem.org/Team:Calgary/1_October_2009 1] [http://2009.igem.org/Team:Calgary/2_October_2009 2] [http://2009.igem.org/Team:Calgary/3_October_2009 3] [http://2009.igem.org/Team:Calgary/4_October_2009 4]
[http://2009.igem.org/Team:Calgary/5_October_2009 5] [http://2009.igem.org/Team:Calgary/6_October_2009 6] [http://2009.igem.org/Team:Calgary/7_October_2009 7] [http://2009.igem.org/Team:Calgary/8_October_2009 8] [http://2009.igem.org/Team:Calgary/9_October_2009 9] [http://2009.igem.org/Team:Calgary/10_October_2009 10] [http://2009.igem.org/Team:Calgary/11_October_2009 11]
[http://2009.igem.org/Team:Calgary/12_October_2009 12] [http://2009.igem.org/Team:Calgary/13_October_2009 13] [http://2009.igem.org/Team:Calgary/14_October_2009 14] [http://2009.igem.org/Team:Calgary/15_October_2009 15] [http://2009.igem.org/Team:Calgary/16_October_2009 16] [http://2009.igem.org/Team:Calgary/17_October_2009 17] [http://2009.igem.org/Team:Calgary/18_October_2009 18]
[http://2009.igem.org/wiki/index.php?title=Team:Calgary/19_October_2009&preload=Team:Calgary/NotebookPreload&action=edit 19] [http://2009.igem.org/wiki/index.php?title=Team:Calgary/20_October_2009&preload=Team:Calgary/NotebookPreload&action=edit 20] [http://2009.igem.org/wiki/index.php?title=Team:Calgary/21_October_2009&preload=Team:Calgary/NotebookPreload&action=edit 21] [http://2009.igem.org/wiki/index.php?title=Team:Calgary/22_October_2009&preload=Team:Calgary/NotebookPreload&action=edit 22] [http://2009.igem.org/wiki/index.php?title=Team:Calgary/23_October_2009&preload=Team:Calgary/NotebookPreload&action=edit 23] [http://2009.igem.org/wiki/index.php?title=Team:Calgary/24_October_2009&preload=Team:Calgary/NotebookPreload&action=edit 24] [http://2009.igem.org/wiki/index.php?title=Team:Calgary/25_October_2009&preload=Team:Calgary/NotebookPreload&action=edit 25]
[http://2009.igem.org/wiki/index.php?title=Team:Calgary/26_October_2009&preload=Team:Calgary/NotebookPreload&action=edit 26] [http://2009.igem.org/wiki/index.php?title=Team:Calgary/27_October_2009&preload=Team:Calgary/NotebookPreload&action=edit 27] [http://2009.igem.org/wiki/index.php?title=Team:Calgary/28_October_2009&preload=Team:Calgary/NotebookPreload&action=edit 28] [http://2009.igem.org/wiki/index.php?title=Team:Calgary/29_October_2009&preload=Team:Calgary/NotebookPreload&action=edit 29] [http://2009.igem.org/wiki/index.php?title=Team:Calgary/30_October_2009&preload=Team:Calgary/NotebookPreload&action=edit 30] [http://2009.igem.org/wiki/index.php?title=Team:Calgary/31_October_2009&preload=Team:Calgary/NotebookPreload&action=edit 31]



JULY 6, 2009


CAROL

Transformation of luxCDABE into both pSB1AK3 and pSB1AC3

  • Ligated luxCDABE into both pSB1AK3 and pSB1AC3
  • Transformed ligated products into Top 10 cells and plated on AK plates for overnight incubation.


CHINMOYEE

Search for parameter values

Looked through many paper for estimates of initial values for each species in our model and estimates for the k values present in the model.
cell volume : 0.58 -0.69 um^3
Kubitschek HE. Cell volume increase in Escherichia coli after shifts to richer media. J Bacteriol. 1990 Jan172(1):94-101
RNA polymerase transcription rate :70nt/sec
Mutation per base pair per replication : Drake JW, A constant rate of spontaneous mutation in DNA-based microbes.Proc Natl Acad Sci U S A. 1991 Aug 1588(16):7160-4 Table 1


EMILY

Construction Begins!

Now that we have our gene of interest, LuxOD47E, Biobricked, we can start construction to add the J13002 promoter and the B0015 terminator. We'll do two constructions in parallel and see if one of them works.

* Did a restriction digest cutting the insert with EcoRI and SpeI and cutting the recipient with EcoRI and XbaI restriction enzymes.

* Performed Antarctic Phosphatase Treatment (NEB) on the recipients, followed by ligation of the inserts and recipients.

*Transformed J13002-LuxOD47E into TOP10 cells and plated on AC and C plates in 25 uL and 50 uL, left plates for overnight growth.

*Did restreaks and made overnight cultures of colony2X LuxOD47E BBK.

FAHD

Marketing and Ethics for July 6th 2009

Today, I e-mailed out our sponsorship package to Shell Canada, Western Canada Oil Spill Cleanups, ConocoPhillips and Syncrude Inc. I will make follow-up calls to them next week. I also continued to work on the July newsletter.

I also read some ethics papers regarding open sources VS closed sources.


JAMIE

Sequencing Results Analysis!

Sequencing results indicated successful construction of the luxOU construct. Glycerol stocks made.


KEVIN

1. Plasmid Isolation of fluorescent proteins

Isolation of fluorescent proteins were done in order to verify the presence and functionality of fluorescent proteins and promoters.

1. Testing of fluorescent proteins and the promoters

Isolated Fluorescent proteins were constructed behind of R0040 or J13002, depending on whether or not the fluorescent protein contained RBS, and transformed into TOP10 cells.


MANDY

Building Frenzy ala Second Life

Some more building design for the labs, platforms, etc. was done today.


PATRICK

Half Way Point: Taking Stock

Retrospective Notebook: This entry was not written on this day, but derived later from working notes I made that day.

The major systems I had built to date included:

  • DNA finding each other in polymer, communicating with RNAP
  • binding/unbinding of TetR with each other, and DNA, and control by the HUD
  • The HUD display system

The messages passed between these parts were beginning to crowd the chat channels I was using, I came up with a few rules of thumb to keep things sane. Each script state gets its own channel to listen on instead of using a single channel for everything, and wherever possible messages from one part to another use the same format: 'owner's ID','destination object's id','message label' as the first three parts of the message. Some messages don't need any more information than that, and some are broadcast messages that don't need a destination object id either. Others would have several additional pieces of information, each separated by a comma. This system has been very reliable, and it takes practically no effort to set up reliable new messages types now.

I believe I actually made a big diagram of all the messages to make sure that none were conflicting with each other, before realizing that if I just followed these rules there couldn't be any problems!

Also made a list of what I hadn't implemented yet:

  • Count mode, aka the Interaction Checker
  • Polymer maker, aka the Biobricker (later fully implemented! wohoo!)
  • DNA binding interactions, I had RNAP and repression working, but not translation/transcription or activation, and the system for termination was a little unstable too. (later fully rectified and implemented as well. yay!)

I also wrote a [http://igemcalgary.blogspot.com/2009/07/welcome-my-name-is-patrick-king-i-am.html blog post] today.


PRIMA

Marketing

Today I spoke to the President from BioAlberta and he generously offered to donate money to iGEM Calgary. BioAlberta was added to our Bronze list for sponsorship.

I also wrote up some emails to companies after researching the companies for the past few days. The rest of the marketing team edited the emails before I sent them off. I also call 8 other companies and 5 companies were interested in looking at our sponsorship package. I sent off the package and updated the iGEM marketing excel spreadsheet. I left a voicemail to the 3 companies and I'll follow up with them tomorrow or later this week. I got the contact information of the Marketing Lead for one of those 3 companies. My plan is to call the other 2 companies tomorrow to first: get a hold of someone in-charge of sponsorship and second: speak to them about our project, potential applications and how it may benefit their company.


STEFAN

Scripting progress

I have gotten some books from the library on scripting. I'm learning by using bits ans pieces from other people's free scripts and modifying them to do different things. I have managed to make a script that is adjustable to make an object move around randomly.


VICKI

Sequencing results from the J13002 + LuxO D47A + B0015 on pSB1AK3 that I prepared on Thursday

The sequencing results were returned from the samples of J13002 + LuxO D47A + B0015 on pSB1AK3 that I sent down last day. These were confirmed positive. I have included the sequencing results for one of the samples and the alignments immediately thereafter. These results are the same for both of the samples that I tested, indicating that the colonies that I tested have the proper sequence for J13002 + LuxO D47A + B0015 on pSB1AK3.



J13002LuxOD47AB0015sequence.jpg

Alignment results



J13002LuxOD47AB0015comparison.jpg


Retrieved from "http://2009.igem.org/Team:Calgary/6_July_2009"