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Template:Team:KULeuven/17 August 2009/VanillinReceptor
From 2009.igem.org
(Difference between revisions)
(New page: * A failed again so we decided to work only with W instead of performing a new PCR. TOPO for W and Z worked because there were colonies visible. White colonies were plated out. * Fluid cul...) |
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* A failed again so we decided to work only with W instead of performing a new PCR. TOPO for W and Z worked because there were colonies visible. White colonies were plated out. | * A failed again so we decided to work only with W instead of performing a new PCR. TOPO for W and Z worked because there were colonies visible. White colonies were plated out. | ||
| - | * Fluid cultures of G2 and G3 were miniprepped | + | * Fluid cultures of G2 and G3 were miniprepped, nanodropped and cut with Dpn I to exclude the motherstrain |
* Mutagenesis 2 on R on position 427 was performed with PCR | * Mutagenesis 2 on R on position 427 was performed with PCR | ||
Latest revision as of 12:44, 26 August 2009
- A failed again so we decided to work only with W instead of performing a new PCR. TOPO for W and Z worked because there were colonies visible. White colonies were plated out.
- Fluid cultures of G2 and G3 were miniprepped, nanodropped and cut with Dpn I to exclude the motherstrain
- Mutagenesis 2 on R on position 427 was performed with PCR
