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August/24 August 2009
From 2009.igem.org
(Difference between revisions)
(New page: 1. '''LB+Amp plate''' <br> <br> 2.'''Min prep'''<br> Result of Nanodrop concentration check<br> (sample No.) (concentration) 7-1 27.8 ng/uL 8-1 23.2 ...) |
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| Line 11: | Line 11: | ||
8-1 23.2 | 8-1 23.2 | ||
| - | 9 | + | 9 21.1 |
10-A 29.1 | 10-A 29.1 | ||
10-B 25.1 | 10-B 25.1 | ||
| Line 23: | Line 23: | ||
(sample No.) (no. of colonies) | (sample No.) (no. of colonies) | ||
| - | 1-4E ~5 | + | [http://partsregistry.org/wiki/index.php?title=Part:BBa_C0051 1-4E] ~5 |
| - | 1-8E 0 | + | [http://partsregistry.org/wiki/index.php?title=Part:BBa_R1051 1-8E] 0 |
| - | 2-16L 1 | + | [http://partsregistry.org/wiki/index.php?title=Part:BBa_K118005 2-16L] 1 |
| - | 2-16N ~15 | + | [http://partsregistry.org/wiki/index.php?title=Part:BBa_K118006 2-16N] ~15 |
| - | 2-18H 2 | + | [http://partsregistry.org/wiki/index.php?title=Part:BBa_K118014 2-18H] 2 |
ligation sample | ligation sample | ||
| Line 55: | Line 55: | ||
↓<br> | ↓<br> | ||
gel electrophoresis<br> | gel electrophoresis<br> | ||
| + | [[Image:Osaka090824.jpg]] | ||
| - | + | result | |
| - | result | + | |
| - | + | 7 (RBS+cinR) | |
| - | 7 (RBS+cinR) | + | |
7-1 OK | 7-1 OK | ||
| - | + | ||
| - | 8 (RBS+LuxI) | + | 8 (RBS+LuxI) |
8-1 OK | 8-1 OK | ||
| - | + | ||
| - | 9 (RBS+LasR+terminater) | + | 9 (RBS+LasR+terminater) |
9 fialure? | 9 fialure? | ||
| - | + | ||
| - | 10 (RBS+EspE+terminater) | + | 10 (RBS+EspE+terminater) |
10 ??? | 10 ??? | ||
| - | + | ||
| - | 11(p(tetR)+RBS+GFP+terminater) | + | 11(p(tetR)+RBS+GFP+terminater) |
11-B OK | 11-B OK | ||
| + | |||
| + | No. 9 10 → ligation rapid check | ||
| + | |||
| + | [https://2009.igem.org/Team:Osaka/NOTES back to NOTES] | ||
Latest revision as of 08:08, 12 October 2009
1. LB+Amp plate
2.Min prep
Result of Nanodrop concentration check
(sample No.) (concentration)
7-1 27.8 ng/uL
8-1 23.2
9 21.1
10-A 29.1
10-B 25.1
11-A 15.7
11-B 34.1
3.transformed check
Checked the cell cultures transformed and plated out yesterday (8/23) for colony formation and made an approximate count of the number of colonies.
(sample No.) (no. of colonies)
[http://partsregistry.org/wiki/index.php?title=Part:BBa_C0051 1-4E] ~5
[http://partsregistry.org/wiki/index.php?title=Part:BBa_R1051 1-8E] 0
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K118005 2-16L] 1
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K118006 2-16N] ~15
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K118014 2-18H] 2
ligation sample
12 0
13 0
14 0
15 0
5 0
to iquid medium(5mL + Amp 5uL)
4.ligation check
| sample | 5 |
| XbaI | 1 |
| PstI | 1 |
| No.2 | 2 |
| dH2O | 11 |
| total | 20uL |
↓
37 , 2hr
↓
gel electrophoresis
result 7 (RBS+cinR) 7-1 OK 8 (RBS+LuxI) 8-1 OK 9 (RBS+LasR+terminater) 9 fialure? 10 (RBS+EspE+terminater) 10 ??? 11(p(tetR)+RBS+GFP+terminater) 11-B OK
No. 9 10 → ligation rapid check
