Template:Team:KULeuven/20 August 2009/VanillinReceptor

From 2009.igem.org

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(New page: * Continued with ethanol precipitation, electroporation and outplating (of R mutation 202))
 
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* Continued with ethanol precipitation, electroporation and outplating (of R mutation 202)
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* Restriction digest of X, Y and {{kulpart|BBa_K145201}} with EcoR1 and Pst1
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* Gelelectrophoresis to check fragments and gelextraction
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* Fluid medium from outplated cells (overgrowth)
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* TOPO-cloning (with new protocol from Invitrogen) for W and Z

Latest revision as of 09:25, 14 September 2009

  • Restriction digest of X, Y and with EcoR1 and Pst1
  • Gelelectrophoresis to check fragments and gelextraction
  • Fluid medium from outplated cells (overgrowth)
  • TOPO-cloning (with new protocol from Invitrogen) for W and Z