Team:British Columbia

From 2009.igem.org

(Difference between revisions)
(→E.coli Traffic Light: A flexible, modular, and transparent system for multi-level assessment of variable inputs.')
 
(51 intermediate revisions not shown)
Line 1: Line 1:
-
<!-- *** What falls between these lines is the Alert Box!  You can remove it from your pages once you have read and understood the alert *** -->
+
{{Template:UBCiGEM2009_menu_home}}
 +
=<font color="#00CC66">E.coli</font> <font color="#FFCC66">Traffic</font> <font color="#FF0000">Light</font>: <br> A ''flexible'', ''modular'', and ''transparent'' system for multi-level assessment of variable inputs.'=
 +
Biosensors have a diverse variety of real-world functions, ranging from measuring blood glucose levels in diabetes patients to assessing environmental contamination of trace toxins. The majority of these sensors are highly specific for a single input, and their outputs often require specialized equipment such as surface plasmon resonance chips.
 +
 
 +
Our project aims to create a biosensor that recognizes a specific target and alters its output fluorescence from green, to yellow, to red as a function of concentration up to critical levels (hence, a biological "traffic light").
 +
 
 +
 
 +
Click the colours of the traffic light to learn about its different subparts!
 +
<!-- [[Image:BritishColumbia-Trafficlight.png|center|400px]] -->
<html>
<html>
-
<div id="box" style="width: 700px; margin-left: 137px; padding: 5px; border: 3px solid #000; background-color: #fe2b33;">
 
-
<div id="template" style="text-align: center; font-weight: bold; font-size: large; color: #f6f6f6; padding: 5px;">
 
-
This is a template page. READ THESE INSTRUCTIONS.
 
-
</div>
 
-
<div id="instructions" style="text-align: center; font-weight: normal; font-size: small; color: #f6f6f6; padding: 5px;">
 
-
You are provided with this team page template with which to start the iGEM season.  You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki.  You can find some examples <a href="https://2009.igem.org/Help:Template/Examples">HERE</a>.
 
-
</div>
 
-
<div id="warning" style="text-align: center; font-weight: bold; font-size: small; color: #f6f6f6; padding: 5px;">
 
-
You <strong>MUST</strong> have a team description page, a project abstract, a complete project description, and a lab notebook.  PLEASE keep all of your pages within your teams namespace. 
 
-
</div>
 
-
</div>
 
-
</html>
 
-
<!-- *** End of the alert box *** -->
 
 +
<img src="/wiki/images/thumb/f/fc/E_coli_Traffic_Light_General.png/950px-E_coli_Traffic_Light_General.png" width=950 usemap="#trafficlight">
 +
 +
<map name="trafficlight">
 +
<area shape="circle"
 +
      coords="774,121,54"
 +
      alt="Sensors: This links to the pBAD promoters that sense the arabinose."
 +
      title="Sensors: This links to the pBAD promoters that sense the arabinose."
 +
      href="/Team:British_Columbia/pBAD"
 +
/>
 +
 +
<area shape="circle"
 +
      coords="775,236,55"
 +
      alt="Lock and Key: this controls when the colors are produced"
 +
      title="Lock and Key: this controls when the colors are produced"
 +
      href="/Team:British_Columbia/LockandKey"
 +
/>
 +
 +
<area shape="circle"
 +
      coords="774,350,54"
 +
      alt="The Jammer: this mRNA sequence blocks the key from opening the lock."
 +
      title="The Jammer: this mRNA sequence blocks the key from opening the lock."
 +
      href="/Team:British_Columbia/Jammer"
 +
/>
 +
 +
</map>
 +
</html>
-
{|align="justify"
 
-
|You can write a background of your team here.  Give us a background of your team, the members, etc.  Or tell us more about something of your choosing.
 
-
|[[Image:Example_logo.png|200px|right|frame]]
 
-
|-
 
-
|
 
-
''Tell us more about your project.  Give us background.  Use this is the abstract of your project.  Be descriptive but concise (1-2 paragraphs)''
 
-
|[[Image:Team.png|right|frame|Your team picture]]
 
-
|-
 
-
|
 
-
|align="center"|[[Team:Example | Team Example]]
 
-
|}
 
-
<!--- The Mission, Experiments --->
 
-
{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"
+
<!--
-
!align="center"|[[Team:British_Columbia|Home]]
+
[[Image:E_coli_Traffic_Light_General.png|thumb|center|950px|Schematic black-box representation of the E. coli Biosensor that detects various concentration inputs and color outputs. The idea is discrete analog outputs based on a user-specified threshold for each range of concentration.]]
-
!align="center"|[[Team:British_Columbia/Team|The Team]]
+
-->
-
!align="center"|[[Team:British_Columbia/Project|The Project]]
+
-
!align="center"|[[Team:British_Columbia/Parts|Parts Submitted to the Registry]]
+
-
!align="center"|[[Team:British_Columbia/Modeling|Modeling]]
+
-
!align="center"|[[Team:British_Columbia/Biosensor_Sensitivity|Biosensor Sensitivity Notebook]]
+
-
!align="center"|[[Team:British_Columbia/Biosensor_Logic_Gate|Biosensor Logic Gate Notebook]]
+
-
|}
+
-
(''Or you can choose different headings.  But you must have a team page, a project page, and a notebook page.'')
+

Latest revision as of 03:58, 22 October 2009

E.coli Traffic Light:
A flexible, modular, and transparent system for multi-level assessment of variable inputs.'

Biosensors have a diverse variety of real-world functions, ranging from measuring blood glucose levels in diabetes patients to assessing environmental contamination of trace toxins. The majority of these sensors are highly specific for a single input, and their outputs often require specialized equipment such as surface plasmon resonance chips.

Our project aims to create a biosensor that recognizes a specific target and alters its output fluorescence from green, to yellow, to red as a function of concentration up to critical levels (hence, a biological "traffic light").


Click the colours of the traffic light to learn about its different subparts!

Sensors: This links to the pBAD promoters that sense the arabinose. Lock and Key: this controls when the colors are produced The Jammer: this mRNA sequence blocks the key from opening the lock.



Retrieved from "http://2009.igem.org/Team:British_Columbia"