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Uppsala-Sweden/3 September 2009
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Karl.brune (Talk | contribs) (New page: {{Uppsala-Sweden Template}} ==Transformation from registry plates== Top 10 cells from rack 10 (-80C) Thaw on ice for 30 minutes Re-suspend DNA in 15µl of dH20 Add 1µl of DNA to TOP10 ...) |
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B0034-Yadh2 (pSB1AK3) | B0034-Yadh2 (pSB1AK3) | ||
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| + | ==Measurement of RBS-Adh2Y construct== | ||
| + | 100 times dilution: [[Media:Plasmidprep_090904.txt]] | ||
Latest revision as of 12:42, 4 September 2009
Transformation from registry plates
Top 10 cells from rack 10 (-80C)
Thaw on ice for 30 minutes Re-suspend DNA in 15µl of dH20 Add 1µl of DNA to TOP10 µl stir gently Let sit for 30min (I just did for 10min)
Put on SOC @ RT for 60min (Just did this for 5min; not so good idea with kan or ch as selective antibiotic but amp works fine) Transfer to plate or liquid culture
Inoculation ON cultures
B0034-Yadh2 (pSB1AK3)
Measurement of RBS-Adh2Y construct
100 times dilution: Media:Plasmidprep_090904.txt
