Team:Chiba/Notebook/Calendar/7 September 2009

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(New page: *AHL水溶液の作成 100 mM AHL水溶液から、10 μM, 1 μM, 1 mM のAHL水溶液を作成した。 *プレートの作成 先ほど作成した各濃度のAHLを用いて、各濃...)
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*AHL水溶液の作成
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100 mM AHL水溶液から、10 μM, 1 μM, 1 mM のAHL水溶液を作成した。
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>[[Team:Chiba/Notebook|Go to the Notebook page]]
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*プレートの作成
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([[Team:Chiba/Notebook/Calendar/6_September_2009|6_September_2009]] <|>[[Team:Chiba/Notebook/Calendar/8_September_2009|8_September_2009]])
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先ほど作成した各濃度のAHLを用いて、各濃度２枚ずつプレートを作成した。
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== Measurement of Receiver's Responce by AHL concentration (1)==
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*Making AHL water solution
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We made 1 &mu;M, 10 &mu;M, 1 mM AHL water solution from 100 mM AHL water solution.
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*プレ培
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BW25113株（ΔTdk, pCIA3-LuxR, plux-GFP）をプレ培した。
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*Making plates
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Using each concentration of AHL water solutions which made ealier, we made two plates per the concentration.
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*prior culture
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We did prior culture using glycerol stock(BW25113, &Delta;Tdk,pCIA3-LuxR,pLux-GFP) .
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== Handwritten Lab Notebook ==
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[[Image:Chiba_Labwork_07Sep09.jpg|200px]]

Latest revision as of 17:50, 23 September 2009

>Go to the Notebook page

(6_September_2009 <|>8_September_2009)

Measurement of Receiver's Responce by AHL concentration (1)

  • Making AHL water solution

We made 1 μM, 10 μM, 1 mM AHL water solution from 100 mM AHL water solution.


  • Making plates

Using each concentration of AHL water solutions which made ealier, we made two plates per the concentration.


  • prior culture

We did prior culture using glycerol stock(BW25113, ΔTdk,pCIA3-LuxR,pLux-GFP) .


Handwritten Lab Notebook

Chiba Labwork 07Sep09.jpg

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