Team:UNIPV-Pavia/Methods Materials/Transformation

(Difference between revisions)

Latest revision as of 10:26, 21 October 2009

Protocols

(estimated time: 3 hours and 30 min + 12-16 hours overnight incubation)

Put 1-2 µl of DNA resuspension or ligation into thaw TOP10 cells tube (every tube contains approximately 60 µl of competent cells).
Incubate on ice for 30 min.
Heat shock: 42°C for 1 min.
Put transformed TOP10 tube on ice and then add 250 µl SOC medium.
Incubate 1 hour at 37°C, 220 rpm.
Plate 200 µl of the solution on a proper agar plate.
Incubate overnight at 37°C.

INGREDIENTS:

LB agar plates with proper antibiotic

Invitrogen TOP10 cells

Resuspended DNA

SOC medium

@@ Line 4: / Line 4: @@
    <tr>
 	<td align="center">
-	<img width="100%" height="180px" src="https://static.igem.org/mediawiki/2009/c/c2/UNIPV_title_MandM.jpg" ></img>
+	<img width="100%" height="180px" src="https://static.igem.org/mediawiki/2009/d/de/UNIPV_title_M%26M.jpg" ></img>
 	</td>
    </tr>
@@ Line 21: / Line 21: @@
       <font face="Pristina" size="50" ><b>Protocols</b></font>
      </td>
+    <td width="50px"></td>
 </html>
    </tr>
@@ Line 42: / Line 43: @@
 *Incubate overnight at 37°C.
 <br><br><br><br><br><br>
-</td><td align='center' valign='bottom' width='300px'>
+<br></td><td align='center' valign='top'  width='300px'>
-<div align='center'>
 <table border='1'>
+<tr bgcolor='yellow'><td>INGREDIENTS:
+</td></tr>
 <tr><td>
-*'''LB agar plates with proper antibiotic'''
+<font class='label'>
-*'''Invitrogen TOP10 cells'''
+'''LB agar plates with proper antibiotic'''<hr color='white' width='70%'>
-*'''Resuspended DNA'''
+'''Invitrogen TOP10 cells'''<hr color='white' width='70%'>
-*'''SOC medium'''
+'''Resuspended DNA'''<hr color='white' width='70%'>
-</td></tr></table></div><br><br><br><br>
+'''SOC medium'''</font>
+</td></tr></table><br><br><br><br>
 </td></tr></table>