"
Template:Team:KULeuven/3 August 2009/VanillinProduction
From 2009.igem.org
(Difference between revisions)
| Line 1: | Line 1: | ||
| - | We received the | + | We received the ''COMT, sam5'' and ''sam8'' from Edinburgh today. Unfortunately, the tube containing the ''sam5'' + RBS (with the PstI site) was opened during transport and all DNA in it was lost. The others were transferred into competent cells by electroporation. |
| - | * | + | * ''sam5'' + RBS in {{kulpart|pSB1A2}} (with PstI site removed), {{kulpart|BBa_K238007}} |
| - | * | + | * ''sam8'' + RBS in {{kulpart|pSB1A2}}, {{kulpart|BBa_I742106}} |
| - | * | + | * ''sam8'' in {{kulpart|pSB1A2}} coding sequence, {{kulpart|BBa_I742142}} |
| - | * | + | * ''COMT'' in {{kulpart|pSB1A2}} coding sequence, {{kulpart|BBa_I742107}} |
| - | The cells were put | + | The cells were put into liquid LB to recuperate from the procedure, transferred onto LB/amp plates and grown overnight at 37°C. |
| - | We also made liquid cultures of the RBS + fcs and RBS + ech cultures so | + | We also made liquid cultures of the RBS + ''fcs'' and RBS + ''ech'' cultures so we can miniprep them tomorrow. |
Latest revision as of 09:46, 14 September 2009
We received the COMT, sam5 and sam8 from Edinburgh today. Unfortunately, the tube containing the sam5 + RBS (with the PstI site) was opened during transport and all DNA in it was lost. The others were transferred into competent cells by electroporation.
- sam5 + RBS in (with PstI site removed),
- sam8 + RBS in ,
- sam8 in coding sequence,
- COMT in coding sequence,
The cells were put into liquid LB to recuperate from the procedure, transferred onto LB/amp plates and grown overnight at 37°C.
We also made liquid cultures of the RBS + fcs and RBS + ech cultures so we can miniprep them tomorrow.
