Template:Team:KULeuven/24 August 2009/BlueLightReceptor

(Difference between revisions)

Latest revision as of 12:25, 14 September 2009

Plates with LigA were put under blue light. The LEDs were put on their max capacity.
Restriction digest with
- tubes (1,3,5,7,9) of LigA (BLP + ) cut with EcoRI en PstI
- promotor cut with EcoRI en SpeI (4x)
Gel electrophoresis with the RD of LigA and followed by a gel extraction:
- Note: tube 5 of LigA was loaded poorly on the gel and could not be used.
- Note: the samples of promotor were barely visible. Only 2 of the 4 samples were recovered by extraction.

4. Enting of liquid cultures with kanamycin and

@@ Line 1: / Line 1: @@
-# Plates with ligA were put under blue light. the LEDs were put on their max capacity.
+# Plates with LigA were put under blue light. The LEDs were put on their max capacity.
 # Restriction digest with
-#*tubes (1,3,5,7,9) of ligA (BLP + {{kulpart|BBa_E0240}}) cut with EcoRI en PstI
+#*tubes (1,3,5,7,9) of LigA (BLP + {{kulpart|BBa_E0240}}) cut with EcoRI en PstI
 #*promotor {{kulpart|BBa_J23101}} cut with EcoRI en SpeI (4x)
-#Gel electroforesis with the RD of ligA and {{kulpart|BBa_J23101}} followed by a gel extraction:
+#Gel electrophoresis with the RD of LigA and {{kulpart|BBa_J23101}} followed by a gel extraction:
-#* Note: tube 5 of ligA was loaded poorly on the gel and could not be used.
+#* Note: tube 5 of LigA was loaded poorly on the gel and could not be used.
 #* Note: the samples of promotor {{kulpart|BBa_J23101}} were barely visible. Only 2 of the 4 samples were recovered by extraction.
 {| border ="1" align="center"
 !| Part || concentration (ng/&mu;l) || 260/280 &lambda; ||
 |- align="center"
-| ligA 1 ||7,9 || 2,16 ||
+| LigA 1 ||7,9 || 2,16 ||
 |- align="center"
-| ligA 3 ||10,8 || 1,88 ||
+| LigA 3 ||10,8 || 1,88 ||
 |- align="center"
-| ligA 7 ||4,8 || 2,49 ||
+| LigA 7 ||4,8 || 2,49 ||
 |- align="center"
-| ligA 9 ||8,0 || 3,02 ||
+| LigA 9 ||8,0 || 3,02 ||
 |- align="center"
 | {{kulpart|BBa_J23101}} (A) ||20,8|| 1,70 ||