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Team:Chiba/Notebook/Calendar/2 September 2009
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>[[Team:Chiba/Notebook|Go to the Notebook page]] | >[[Team:Chiba/Notebook|Go to the Notebook page]] | ||
| - | [[Team:Chiba/Notebook/Calendar/2_September_2009|Lab Work Start!]] |>[[Team:Chiba/Notebook/Calendar/3_September_2009|3_September_2009]] | + | ([[Team:Chiba/Notebook/Calendar/2_September_2009|Lab Work Start!]] |>[[Team:Chiba/Notebook/Calendar/3_September_2009|3_September_2009]]) |
| + | <html><link rel="stylesheet" href="https://2008.igem.org/wiki/index.php?title=User:Maiko/chiba.css&action=raw&ctype=text/css" type="text/css" /></html> | ||
| - | + | == Making Fluorescent Transfer Curve for the Practice of Lab Work (Day 1)== | |
| + | *prior culture | ||
| - | + | medium:liquid culture medium antibiotic:Amp | |
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| - | |||
| - | |||
| + | We took this medium to centrifuging tube 50 mL and added antibiotic(Cm) 50 μL. | ||
| + | |||
| + | Then we transferred the medium to test tube 2 mL and added glycerol stock(BW25113, ΔTdk,pCIA3-LuxR,pLux-GFP) and then started shake culture at 37 degrees Celsius. | ||
| + | (21:40-) | ||
| + | == Handwritten Lab Notebook == | ||
| - | [[Image:20090903-1.jpg|200px]][[Image:Chiba_Labwork_02Sep09.jpg|200px]] | + | [[Image:20090903-1.jpg|200px]] [[Image:Chiba_Labwork_02Sep09.jpg|200px]] |
Latest revision as of 17:42, 23 September 2009
(Lab Work Start! |>3_September_2009)
Making Fluorescent Transfer Curve for the Practice of Lab Work (Day 1)
- prior culture
medium:liquid culture medium antibiotic:Amp
We took this medium to centrifuging tube 50 mL and added antibiotic(Cm) 50 μL.
Then we transferred the medium to test tube 2 mL and added glycerol stock(BW25113, ΔTdk,pCIA3-LuxR,pLux-GFP) and then started shake culture at 37 degrees Celsius. (21:40-)
Handwritten Lab Notebook
