Team:Chiba/Notebook/Calendar/2 September 2009

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Latest revision as of 17:42, 23 September 2009

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(Lab Work Start! |>3_September_2009)

Making Fluorescent Transfer Curve for the Practice of Lab Work (Day 1)

prior culture

medium:liquid culture medium antibiotic:Amp

We took this medium to centrifuging tube 50 mL and added antibiotic(Cm) 50 μL.

Then we transferred the medium to test tube 2 mL and added glycerol stock(BW25113, ΔTdk,pCIA3-LuxR,pLux-GFP) and then started shake culture at 37 degrees Celsius. (21:40-)

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-*プレ培養(予備実験)/prior culture(exploratory experiment)
+== Making Fluorescent Transfer Curve for the Practice of Lab Work (Day 1)==
+*prior culture
-使った培地：Amp耐性、液体/medium:liquid culture medium  antibiotic:Amp
+medium:liquid culture medium  antibiotic:Amp
-この溶液50 mLを遠心管にとり、更に抗生物質(Cm)を50 μLを加え、Amp-Cm液体培地とした。/We took this medium to centrifuging tube 50 mL and added antibiotic(Cm) 50 uL.
-これを試験管に2 mLとり、グリセロールストック(BW25113株、Δtdk、pcIA3-LuxR、pLux-GFP)を添加し37℃で振とうした。(21:40-)/Then we transferred the medium to test tube 2 mL and added glycerol stock(BW25113, ΔTdk,pCIA3-LuxR,pLux-GFP) then started shake culture at 37 degrees Celsius.
+We took this medium to centrifuging tube 50 mL and added antibiotic(Cm) 50 &mu;L.
+Then we transferred the medium to test tube 2 mL and added glycerol stock(BW25113, &Delta;Tdk,pCIA3-LuxR,pLux-GFP) and then started shake culture at 37 degrees Celsius.
+(21:40-)
+== Handwritten Lab Notebook ==
 [[Image:20090903-1.jpg|200px]]  [[Image:Chiba_Labwork_02Sep09.jpg|200px]]