Team:Chiba/Notebook/Calendar/7 September 2009

(Difference between revisions)

Latest revision as of 17:50, 23 September 2009

We made 1 μM, 10 μM, 1 mM AHL water solution from 100 mM AHL water solution.

Using each concentration of AHL water solutions which made ealier, we made two plates per the concentration.

We did prior culture using glycerol stock(BW25113, ΔTdk,pCIA3-LuxR,pLux-GFP) .

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+== Measurement of Receiver's Responce by AHL concentration (1)==
+*Making AHL water solution
+We made 1 &mu;M, 10 &mu;M, 1 mM AHL water solution from 100 mM AHL water solution.
+*Making plates
+Using each concentration of AHL water solutions which made ealier, we made two plates per the concentration.
-*AHL水溶液の作成
+*prior culture
-mM AHL水溶液から、10 μM, 1 μM, 1 mM のAHL水溶液を作成した。
+We did prior culture using glycerol stock(BW25113, &Delta;Tdk,pCIA3-LuxR,pLux-GFP) .
-*プレートの作成
-先ほど作成した各濃度のAHLを用いて、各濃度２枚ずつプレートを作成した。
+== Handwritten Lab Notebook ==
-*プレ培
+[[Image:Chiba_Labwork_07Sep09.jpg|200px]]
-BW25113株（ΔTdk, pCIA3-LuxR, plux-GFP）をプレ培した。