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Team:Chiba/Notebook/Calendar/9 September 2009
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([[Team:Chiba/Notebook/Calendar/8_September_2009|8_September_2009]] <|>[[Team:Chiba/Notebook/Calendar/10_September_2009|10_September_2009]]) | ([[Team:Chiba/Notebook/Calendar/8_September_2009|8_September_2009]] <|>[[Team:Chiba/Notebook/Calendar/10_September_2009|10_September_2009]]) | ||
| + | == Check of Sender's AHL generation (1) == | ||
| + | === 08:00 main culture === | ||
| + | :*divided injection 12.5ml of the culture to 10 50ml centrifuging tubes | ||
| + | :*WASH 3 times | ||
| + | :*Add 12.5ml new culture | ||
| + | :*Centrifuge the 1st tube 3000rpm 5min 20℃ and pick the upernatant | ||
| + | :*Do same operation every 15min to 2nd~10th tubes | ||
| + | :*after all, mix the supernatant and solid media at 1:1 | ||
| + | :*make 10 concentration varieties of solid media | ||
| + | |||
| + | == Handwritten Lab Notebook == | ||
[[Image:Chiba_Labwork_09Sep09.jpg|200px]] [[Image:Chiba_Labwork_09Sep09-2.jpg|200px]] | [[Image:Chiba_Labwork_09Sep09.jpg|200px]] [[Image:Chiba_Labwork_09Sep09-2.jpg|200px]] | ||
Latest revision as of 18:25, 23 September 2009
(8_September_2009 <|>10_September_2009)
Check of Sender's AHL generation (1)
08:00 main culture
- divided injection 12.5ml of the culture to 10 50ml centrifuging tubes
- WASH 3 times
- Add 12.5ml new culture
- Centrifuge the 1st tube 3000rpm 5min 20℃ and pick the upernatant
- Do same operation every 15min to 2nd~10th tubes
- after all, mix the supernatant and solid media at 1:1
- make 10 concentration varieties of solid media
Handwritten Lab Notebook
