Template:Team:KULeuven/10 September 2009/BlueLightReceptor

(Difference between revisions)

Latest revision as of 08:24, 14 September 2009

Since the results from our light experiments keep failing, some new ideas were computed. pSB1A2 is the backbone for our construct but since this is a high copy number plasmid perhaps the ratio of promotor to repressor is unequal (not enough repressor). A new enting in LC was done with ligX. These were grown for a few hours at 37°C and then put in 16°C room overnight so that more repressor can be made (its promotor is temperature-sensitive).
Ligation from 09/08 was electroporated in competent cells.
Miniprep of LigX, restriction digest and gel electrophoresis. The signals were good so a motherplate for ligX was made. Also, the vector pSB1A2 was extracted from the gel (39,1 ng/µl).

Nanodrop results:

@@ Line 1: / Line 1: @@
-# miniprep of LigX
+# Since the results from our light experiments keep failing, some new ideas were computed. pSB1A2 is the backbone for our construct but since this is a high copy number plasmid perhaps the ratio of promotor to repressor is unequal (not enough repressor). A new enting in LC was done with ligX. These were grown for a few hours at 37°C and then put in 16°C room overnight so that more repressor can be made (its promotor is temperature-sensitive).
-nanodrop results:
+# Ligation from 09/08 was electroporated in competent cells.
+# Miniprep of LigX, restriction digest and gel electrophoresis. The signals were good so a motherplate for ligX was made. Also, the vector pSB1A2 was extracted from the gel (39,1 ng/µl).
+Nanodrop results:
 {| border ="1" align="center"
 !| Part || concentration (ng/&mu;l) || 260/280 &lambda; ||