Template:Team:KULeuven/9 September 2009/BlueLightReceptor

From 2009.igem.org

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# FACS-ing the liquid cultures that were put under blue light on 8/09 gave no significant results.  
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# FACS-ing the liquid cultures that were put under blue light on 08/09 gave no significant results.  
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# motherplate for {{kulpart|BBa_J23101}} was made
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# Motherplate for {{kulpart|BBa_J23101}} was made
# liquid cultures for checking the ligX were made.
# liquid cultures for checking the ligX were made.
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# LC with {{kulpart|BBa_J23101}} were miniprepped, nanodropped, restrictie digested and put on gel. the signals were good. the vector, pSB1A2, was cut from the gel and gel extracted. this was ligated with {{kulpart|BBa_K238013}}.
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# LC with {{kulpart|BBa_J23101}} were miniprepped, nanodropped, restriction digested and put on gel. The signals were good. The vector, pSB1A2, was cut from the gel and gel extracted (10ng/µl). This was ligated with {{kulpart|BBa_K238013}} (LigY).
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nanodrop:
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Nanodrop:
{| border ="1" align="center"
{| border ="1" align="center"
!| Part || concentration (ng/μl) || 260/280 λ ||  
!| Part || concentration (ng/μl) || 260/280 λ ||  
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|- align="center"
|- align="center"
| {{kulpart|BBa_J23101}}(4) || 141 || 1,95 ||  
| {{kulpart|BBa_J23101}}(4) || 141 || 1,95 ||  
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|}  
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|}
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gel extractie:
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|- align="center"
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| {{kulpart|BBa_J23101}} || 10,8 ||  ||
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Latest revision as of 08:27, 14 September 2009

  1. FACS-ing the liquid cultures that were put under blue light on 08/09 gave no significant results.
  2. Motherplate for was made
  3. liquid cultures for checking the ligX were made.
  4. LC with were miniprepped, nanodropped, restriction digested and put on gel. The signals were good. The vector, pSB1A2, was cut from the gel and gel extracted (10ng/µl). This was ligated with (LigY).

Nanodrop:

Part concentration (ng/μl) 260/280 λ
(1) 141,8 1,94
(2) 139,5 1,99
(3) 177,5 1,95
(4) 141 1,95
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