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Template:Team:KULeuven/11 September 2009/BlueLightReceptor
From 2009.igem.org
(Difference between revisions)
(New page: # the electroporated ligY did not grow. A new restrictie digest on pcr product {{kulpart|BBa_K238013}} was performed with EcoRI and PstI.) |
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| - | # | + | # The electroporated ligY did not grow. A new restriction digest on pcr product {{kulpart|BBa_K238013}} was performed with EcoRI and PstI.This was put on gel. There was a good signal so it was used to perform a ligation with vector pSB1A2. |
| + | # A liquid culture that was made from pSB3K3 a few days ago showed growth after all. This was miniprepped and restriction digested to check whether the vector is actually present. | ||
| + | # The liquid cultures with our blue light construct (lig) that stood overnight at 16°C was FACS-ed. Half of the cultures were exposed to blue light during the day and put under the FACS in the evening. | ||
Latest revision as of 08:20, 14 September 2009
- The electroporated ligY did not grow. A new restriction digest on pcr product was performed with EcoRI and PstI.This was put on gel. There was a good signal so it was used to perform a ligation with vector pSB1A2.
- A liquid culture that was made from pSB3K3 a few days ago showed growth after all. This was miniprepped and restriction digested to check whether the vector is actually present.
- The liquid cultures with our blue light construct (lig) that stood overnight at 16°C was FACS-ed. Half of the cultures were exposed to blue light during the day and put under the FACS in the evening.
