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Team:Imperial College London/Biobricks
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| - | + | =Submitted BioBricks= | |
| - | = | + | Imperial College iGEM 2009's uploaded BioBricks section on the Parts Registry can be found [http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2009&group=Imperial%20College%20London here].<br><br> |
<!--template:----------------------------------------------------------------------------> | <!--template:----------------------------------------------------------------------------> | ||
| - | {| style="color:#CCC; background-color:# | + | {| style="color:#CCC; background-color:#325d97;" cellpadding="6" cellspacing="0" border="3" |
! Registry Code | ! Registry Code | ||
! Type | ! Type | ||
! Sequence Description | ! Sequence Description | ||
| - | |- style="color:#333; background-color:# | + | |- style="color:#333; background-color:#CCCCFF;" cellpadding="6" cellspacing="0" border="1" |
<!----------------------------------------biobricks----------------------------------------> | <!----------------------------------------biobricks----------------------------------------> | ||
| <partinfo>BBa_K200000 </partinfo> | | <partinfo>BBa_K200000 </partinfo> | ||
| Coding | | Coding | ||
| - | | '''RcsB''' is a receiver protein | + | | '''RcsB''' is a receiver protein which acts as a positive regulator of a number of genes including capsule genes responsible for colanic acid production. |
| - | |- style="color:#333; background-color:# | + | |- style="color:#333; background-color:#CCCCFF;" cellpadding="6" cellspacing="0" border="1" |
| <partinfo>BBa_K200001 </partinfo> | | <partinfo>BBa_K200001 </partinfo> | ||
| Coding | | Coding | ||
| - | | '''Dam (DNA Adenine Methylase)''' The methylase encoded by the dam gene ([http://en.wikipedia.org/wiki/Dam_(methylase) Dam methylase]) transfers a methyl group from S-adenosylmethionine to the N6 position of the adenine residues in the sequence GATC | + | | '''Dam (DNA Adenine Methylase)''' The methylase encoded by the dam gene ([http://en.wikipedia.org/wiki/Dam_(methylase) Dam methylase]) transfers a methyl group from S-adenosylmethionine to the N6 position of the adenine residues in the sequence GATC, this protects the DNA from cleavage. |
| - | |- style="color:#333; background-color:# | + | |- style="color:#333; background-color:#CCCCFF;" cellpadding="6" cellspacing="0" border="1" |
| <partinfo>BBa_K200002 </partinfo> | | <partinfo>BBa_K200002 </partinfo> | ||
| Coding | | Coding | ||
| '''Colanic acid global regulator''' ygiV (B3023) increases the production of colanic acid further in conjunction with RcsB by acting as a repressor for mcbR/yncC promoter. YncC/mcbR normally repress colanic acid overproduction so as to increase biofilm formation. | | '''Colanic acid global regulator''' ygiV (B3023) increases the production of colanic acid further in conjunction with RcsB by acting as a repressor for mcbR/yncC promoter. YncC/mcbR normally repress colanic acid overproduction so as to increase biofilm formation. | ||
| - | |- style="color:#333; background-color:# | + | |- style="color:#333; background-color:#CCCCFF;" cellpadding="6" cellspacing="0" border="1" |
| <partinfo>BBa_K200003 </partinfo> | | <partinfo>BBa_K200003 </partinfo> | ||
| Coding | | Coding | ||
| - | | '''Waal Ligase''' is an enzyme responsible for the ligation of an O-antigen to the core oligosaccharide in the Gram-negative bacterium's outer membrane. | + | | '''Waal Ligase''' is an enzyme responsible for the ligation of an [http://en.wikipedia.org/wiki/O_antigen#O-antigen O-antigen] to the core [http://en.wikipedia.org/wiki/Oligosaccharide oligosaccharide] in the Gram-negative bacterium's outer membrane. |
| - | + | ||
| - | + | |- style="color:#333; background-color:#CCCCFF;" cellpadding="6" cellspacing="0" border="1" | |
| - | |- style="color:#333; background-color:# | + | |
| <partinfo>BBa_K200005 </partinfo> | | <partinfo>BBa_K200005 </partinfo> | ||
| Coding | | Coding | ||
| - | | '''OtsA''' is the first of two required in the conversion of glucose to trehalose. | + | | '''OtsA''' is the first of two required in the conversion of glucose to [http://en.wikipedia.org/wiki/Trehalose trehalose]. |
This enzyme catalyses the following reaction: | This enzyme catalyses the following reaction: | ||
| + | |||
UDP-glucose + D-glucose 6-phosphate -> UDP + alpha,alpha-trehalose 6-phosphate | UDP-glucose + D-glucose 6-phosphate -> UDP + alpha,alpha-trehalose 6-phosphate | ||
| - | |- style="color:#333; background-color:# | + | |- style="color:#333; background-color:#CCCCFF;" cellpadding="6" cellspacing="0" border="1" |
| <partinfo>BBa_K200006 </partinfo> | | <partinfo>BBa_K200006 </partinfo> | ||
| Coding | | Coding | ||
| - | | '''OtsB''' This enzyme is the second of two required for the conversion of glucose to trehalose. | + | | '''OtsB''' This enzyme is the second of two required for the conversion of glucose to [http://en.wikipedia.org/wiki/Trehalose trehalose]. |
This enzyme catalyses the following reaction: | This enzyme catalyses the following reaction: | ||
| + | |||
alpha,alpha-trehalose 6-phosphate + H2O -> alpha,alpha-trehalose + phosphate | alpha,alpha-trehalose 6-phosphate + H2O -> alpha,alpha-trehalose + phosphate | ||
| - | |- style="color:#333; background-color:# | + | |- style="color:#333; background-color:#CCCCFF;" cellpadding="6" cellspacing="0" border="1" |
| <partinfo>BBa_K200007 </partinfo> | | <partinfo>BBa_K200007 </partinfo> | ||
| Coding | | Coding | ||
| - | | '''Cellulase''' mainly catalyses the reactions that changes crystalline cellulose to cellobiose and then finally to glucose | + | | [http://en.wikipedia.org/wiki/Cellulase '''Cellulase'''] mainly catalyses the reactions that changes crystalline [http://en.wikipedia.org/wiki/Cellulose cellulose] to [http://en.wikipedia.org/wiki/Cellobiose cellobiose] and then finally to glucose. This cellulase is protease resistant. |
| - | |- style="color:#333; background-color:# | + | |- style="color:#333; background-color:#CCCCFF;" cellpadding="6" cellspacing="0" border="1" |
| <partinfo>BBa_K200008 </partinfo> | | <partinfo>BBa_K200008 </partinfo> | ||
| Coding | | Coding | ||
| - | | '''Phenylalanine hydroxylase ''' is the enzyme that breaks down [http://en.wikipedia.org/wiki/Phenylalanine phenylalanine] to [http://en.wikipedia.org/wiki/Tyrosine tyrosine]. Deficiency of this enzyme activity results in the autosomal recessive disorder [http://en.wikipedia.org/wiki/Phenylketonuria phenylketonuria]. | + | | [http://en.wikipedia.org/wiki/Phenylalanine_hydroxylase '''Phenylalanine hydroxylase'''] is the enzyme that breaks down [http://en.wikipedia.org/wiki/Phenylalanine phenylalanine] to [http://en.wikipedia.org/wiki/Tyrosine tyrosine]. Deficiency of this enzyme activity results in the autosomal recessive disorder [http://en.wikipedia.org/wiki/Phenylketonuria phenylketonuria]. |
| - | |- style="color:#333; background-color:# | + | |- style="color:#333; background-color:#CCCCFF;" cellpadding="6" cellspacing="0" border="1" |
| + | | <partinfo>BBa_K200009 </partinfo> | ||
| + | | Coding | ||
| + | | '''Restriction enzyme [http://www.thelabrat.com/restriction/DpnII.shtml DpnII] ''' is a Type II restriction enzyme that recognises the sequence GATC. Its activity can be blocked by dam methylation. | ||
| + | |||
| + | |- style="color:#333; background-color:#CCCCFF;" cellpadding="6" cellspacing="0" border="1" | ||
| + | | <partinfo>BBa_K200010 </partinfo> | ||
| + | | Coding | ||
| + | | '''Restriction enzyme [http://www.thelabrat.com/restriction/TaqI.shtml TaqI] ''' is a Type II restriction enzyme that recognises the sequence TCGA. Its activity can be blocked by dam methylation. | ||
| + | |||
| + | |- style="color:#333; background-color:#CCCCFF;" cellpadding="6" cellspacing="0" border="1" | ||
| + | | <partinfo>BBa_K200011 </partinfo> | ||
| + | | Coding | ||
| + | | This '''Lamda cI repressor ''' has a cI857 mutation that results in denaturation of the repressor when the temperature is raised from 30 to 42°C, thereby allowing lambda promoter expression. | ||
| + | |||
| + | When the temperature is raised, typically to 42°C, the functionality of the protein is lost and the cI repressor is no longer able to bind to the operators on its promoter. Therefore, lambda promoter expression increases. | ||
| + | |- style="color:#333; background-color:#CCCCFF;" cellpadding="6" cellspacing="0" border="1" | ||
| + | | <partinfo>BBa_K200012 </partinfo> | ||
| + | | Regulatory | ||
| + | | '''Lambda promoter (cIts responsive) ''' is different from the common lambda promoter in that it is able to be repressed by the temperature sensitive cI protein (BBa_K200011). When it is not being repressed after 42°C induction, it acts as a strong promoter. | ||
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{{Imperial/09/TemplateBottom}} | {{Imperial/09/TemplateBottom}} | ||
Latest revision as of 11:41, 15 September 2009
Submitted BioBricks
Imperial College iGEM 2009's uploaded BioBricks section on the Parts Registry can be found [http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2009&group=Imperial%20College%20London here].
| Registry Code | Type | Sequence Description |
|---|---|---|
| Coding | RcsB is a receiver protein which acts as a positive regulator of a number of genes including capsule genes responsible for colanic acid production. | |
| Coding | Dam (DNA Adenine Methylase) The methylase encoded by the dam gene ([http://en.wikipedia.org/wiki/Dam_(methylase) Dam methylase]) transfers a methyl group from S-adenosylmethionine to the N6 position of the adenine residues in the sequence GATC, this protects the DNA from cleavage. | |
| Coding | Colanic acid global regulator ygiV (B3023) increases the production of colanic acid further in conjunction with RcsB by acting as a repressor for mcbR/yncC promoter. YncC/mcbR normally repress colanic acid overproduction so as to increase biofilm formation. | |
| Coding | Waal Ligase is an enzyme responsible for the ligation of an [http://en.wikipedia.org/wiki/O_antigen#O-antigen O-antigen] to the core [http://en.wikipedia.org/wiki/Oligosaccharide oligosaccharide] in the Gram-negative bacterium's outer membrane. | |
| Coding | OtsA is the first of two required in the conversion of glucose to [http://en.wikipedia.org/wiki/Trehalose trehalose].
This enzyme catalyses the following reaction: UDP-glucose + D-glucose 6-phosphate -> UDP + alpha,alpha-trehalose 6-phosphate | |
| Coding | OtsB This enzyme is the second of two required for the conversion of glucose to [http://en.wikipedia.org/wiki/Trehalose trehalose].
This enzyme catalyses the following reaction: alpha,alpha-trehalose 6-phosphate + H2O -> alpha,alpha-trehalose + phosphate | |
| Coding | [http://en.wikipedia.org/wiki/Cellulase Cellulase] mainly catalyses the reactions that changes crystalline [http://en.wikipedia.org/wiki/Cellulose cellulose] to [http://en.wikipedia.org/wiki/Cellobiose cellobiose] and then finally to glucose. This cellulase is protease resistant. | |
| Coding | [http://en.wikipedia.org/wiki/Phenylalanine_hydroxylase Phenylalanine hydroxylase] is the enzyme that breaks down [http://en.wikipedia.org/wiki/Phenylalanine phenylalanine] to [http://en.wikipedia.org/wiki/Tyrosine tyrosine]. Deficiency of this enzyme activity results in the autosomal recessive disorder [http://en.wikipedia.org/wiki/Phenylketonuria phenylketonuria]. | |
| Coding | Restriction enzyme [http://www.thelabrat.com/restriction/DpnII.shtml DpnII] is a Type II restriction enzyme that recognises the sequence GATC. Its activity can be blocked by dam methylation. | |
| Coding | Restriction enzyme [http://www.thelabrat.com/restriction/TaqI.shtml TaqI] is a Type II restriction enzyme that recognises the sequence TCGA. Its activity can be blocked by dam methylation. | |
| Coding | This Lamda cI repressor has a cI857 mutation that results in denaturation of the repressor when the temperature is raised from 30 to 42°C, thereby allowing lambda promoter expression.
When the temperature is raised, typically to 42°C, the functionality of the protein is lost and the cI repressor is no longer able to bind to the operators on its promoter. Therefore, lambda promoter expression increases. | |
| Regulatory | Lambda promoter (cIts responsive) is different from the common lambda promoter in that it is able to be repressed by the temperature sensitive cI protein (BBa_K200011). When it is not being repressed after 42°C induction, it acts as a strong promoter. | |
