Team:Imperial College London/Drylab

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=[[Image:II09_Drylab.png|80px]]Drylab Hub=
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=[[Image:II09_DryLabThumb.png|80px]]<font size='5'><b>Dry Lab Hub</b></font>=
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The goal of the Drylab has been to support the Wetlab by answering questions of interest.  
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Welcome to the Dry Lab!
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The conclusions from the models will provide the team with functional understanding of the system, and provide design constraints and considerations that should be taken into account in the genetic constructs.  
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<br>
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In the Drylab, we have implemented several functional models, explaining the behaviour of different parts of the system.This section will provide a summary of the models, with the conclusions drawn from them.
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The goal of the Dry Lab has been to support the Wet Lab by answering questions of interest. The primary function of the models is to instruct the data analysis once results are gathered from the lab.
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==Autoinduction==
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Conclusions from the models aim to provide the team with a functional understanding of the system as well as design considerations that should be addressed in the genetic constructs. We have implemented several models, explaining the behaviour of different parts of the system. Below is a summary:<br>
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Switching to secondary carbon sources and diauxic growth
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<!--
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*[https://2009.igem.org/Team:Imperial_College_London/Temporal_Control#Autoinduction Overview]<br>
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*[https://2009.igem.org/Team:Imperial_College_London/Drylab/Autoinduction/Analysis The Model]<br>
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*[https://2009.igem.org/Team:Imperial_College_London/Drylab/Autoinduction/Simulations Simulations]<br>
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==Protein production==
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--[[User:Mabult|Mabult]] 17:05, 17 October 2009 (UTC) Do not forget that your models instruct the data analysis- it is actually their primary function-->
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Production of Protein of interest via LacI-IPTG induction
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*[https://2009.igem.org/Team:Imperial_College_London/M1/Modelling Overview]<br>
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*[https://2009.igem.org/Team:Imperial_College_London/Drylab/M1/Protein_production/Analysis The Model]<br>
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*[https://2009.igem.org/Team:Imperial_College_London/Drylab/M1/Protein_production/Simulations Simulations]<br>
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==Kinetics of protein drug==
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[[Image:II09_drylabhub2.png|720px]]
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Michaelis-Menten enzyme kinetics of protein of interest.
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<!--
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*[https://2009.igem.org/Team:Imperial_College_London/M1/Modelling Overview]<br>
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*<b>Autoinduction:</b>
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*[https://2009.igem.org/Team:Imperial_College_London/Drylab/M1/Enzyme_kinetics/Analysis The Model]<br>
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**This section is responsible for switching on the encapsulation process when glucose in the medium is used up by the cell culture.
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*[https://2009.igem.org/Team:Imperial_College_London/Drylab/M1/Enzyme_kinetics/Simulations Simulations]<br>
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** The goal of the model is to quantify this switch point from a primary to a secondary carbon source and provide an understanding of the diauxie phenomenon.  
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*<b>Protein Production:</b>
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** Production of the drug of interest is controlled by the input amount of IPTG in the system. Prior IPTG induction, production of the drug of interest is repressed by LacI.  
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** The goal of the model is to account for factors that may enhance/reduce the output yield of our drug of interest.
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*<b>Drug Kinetics:</b>
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** The drugs we are producing (PAH, cellulase, opiorphin) are enzymes. In order to detect how much drug has been produced, an understanding of their enzymatic activity is required.  
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** The goal of the model is to better understand the drugs of interest produced using the relationships derived by Michaelis-Menten.
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*<b>Genome Deletion:</b>
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** Genome deletion is induced by an increase in temperature. Different temperatures relate to different restriction enzyme concentrations, so here we explore the possible effects of temperature on cell death.  
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** The goal of the model is to explore a possible relationship between live cells and dead cells over time at different culture temperatures.
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==Genome deletion==
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Killing induced by thermoinduction
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Modelling in this project has been important in defining the "Engineering constraints" of the project, and in particular, we have focused on quantifiying the [https://2009.igem.org/Team:Imperial_College_London/Temporal_Control temporal control].
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*[https://2009.igem.org/Team:Imperial_College_London/M3/Modelling Overview]<br>
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<!--  --[[User:Mabult|Mabult]] 17:10, 17 October 2009 (UTC) repeating yourself here !!!-->
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*[https://2009.igem.org/IGEM:IMPERIAL/2009/M3/Modelling/Model1l The Model]<br>
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*[https://2009.igem.org/Team:Imperial_College_London/Drylab/M3/Thermoinduction/Simulations Simulations]<br>
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<!-- [[Image:m1.fgc.JPG | 400px.jpg]]-->
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<!--
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<html><center><a href="https://2009.igem.org/Team:Imperial_College_London/Genetic_Circuit"><img width=150px src="https://static.igem.org/mediawiki/2009/d/d9/II09_Temp_ArrowLeft.png"></a>
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<a href="https://2009.igem.org/Team:Imperial_College_London/Ethics"><img width=150px src="https://static.igem.org/mediawiki/2009/c/ce/II09_Temp_ArrowRight.png"></a>
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</html>
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Click on the links to find out more!
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<html><center><a href="https://2009.igem.org/Team:Imperial_College_London/Drylab/Autoinduction"><img style="vertical-align:bottom;" width="20%" src="http://i691.photobucket.com/albums/vv271/dk806/II09_Drylabmainimage1.png"></a><a href="https://2009.igem.org/Team:Imperial_College_London/Drylab/Protein_Production"><img style="vertical-align:bottom;" width="20%" src="http://i691.photobucket.com/albums/vv271/dk806/II09_Drylabmainimage2.png"></a><a href="https://2009.igem.org/Team:Imperial_College_London/Drylab/Enzyme"><img style="vertical-align:bottom;" width="20%" src="http://i691.photobucket.com/albums/vv271/dk806/II09_Drylabmainimage3.png"></a>
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<a href="https://2009.igem.org/Team:Imperial_College_London/Drylab/Genome_deletion"><img style="vertical-align:bottom;" width="20%" src="http://i691.photobucket.com/albums/vv271/dk806/II09_Drylabmainimage5.png"></a></center></html>
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<html><table border="0" style="background-color:transparent;" width="100%">
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<tr><td width="0%">&nbsp;</td>
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<td width="22%"><center><a href="https://2009.igem.org/Team:Imperial_College_London/Drylab/Autoinduction"><b>Autoinduction</b></a></center></td>
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<td width="24%"><center><a href="https://2009.igem.org/Team:Imperial_College_London/Drylab/Protein_Production"><b>Protein Production</b></a></center></td>
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<td width="22%"><left><a href="https://2009.igem.org/Team:Imperial_College_London/Drylab/Enzyme"><b>Drug Kinetics</b></a></left></td>
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<td width="22%"><left><a href="https://2009.igem.org/Team:Imperial_College_London/Drylab/Genome_deletion"><b>Genome Deletion</b></a></left></td>
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<td width="1%"></td>
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</tr></table></html>
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<br>
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<!--
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[https://2009.igem.org/Team:Imperial_College_London/Drylab/Autoinduction <b>Autoinduction</b><br>[[Image:II09_diauxi_illust.jpg|100px|left]]]
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[https://2009.igem.org/Team:Imperial_College_London/Drylab/Protein_Production <b>Protein production</b><br>[[Image:II09_NoIPTG_yesIPTG.jpg|100px|left]]]
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[https://2009.igem.org/Team:Imperial_College_London/Drylab/Enzyme <b>Kinetics of protein drug</b><br>[[Image:II09_drylab3.jpg|100px|left]]]
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[https://2009.igem.org/Team:Imperial_College_London/Drylab/Genome_deletion <b>Genome deletion</b><br>[[Image:II09_hitemp_lotemp.jpg|100px|left]]]
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Latest revision as of 02:58, 22 October 2009

II09 DryLabThumb.pngDry Lab Hub

Welcome to the Dry Lab!
The goal of the Dry Lab has been to support the Wet Lab by answering questions of interest. The primary function of the models is to instruct the data analysis once results are gathered from the lab. Conclusions from the models aim to provide the team with a functional understanding of the system as well as design considerations that should be addressed in the genetic constructs. We have implemented several models, explaining the behaviour of different parts of the system. Below is a summary:

II09 drylabhub2.png


Modelling in this project has been important in defining the "Engineering constraints" of the project, and in particular, we have focused on quantifiying the temporal control.



Click on the links to find out more!

 
Autoinduction
Protein Production
 Drug Kinetics Genome Deletion



Mr. Gene   Geneart   Clontech   Giant Microbes

Retrieved from "http://2009.igem.org/Team:Imperial_College_London/Drylab"