"
Team:UNIPV-Pavia/Methods Materials/Ligation
From 2009.igem.org
(Difference between revisions)
(→Ligation) |
(→Ligation) |
||
| (3 intermediate revisions not shown) | |||
| Line 4: | Line 4: | ||
<tr> | <tr> | ||
<td align="center"> | <td align="center"> | ||
| - | <img width="100%" height="180px" src="https://static.igem.org/mediawiki/2009/ | + | <img width="100%" height="180px" src="https://static.igem.org/mediawiki/2009/d/de/UNIPV_title_M%26M.jpg" ></img> |
</td> | </td> | ||
</tr> | </tr> | ||
| Line 21: | Line 21: | ||
<font face="Pristina" size="50" ><b>Protocols</b></font> | <font face="Pristina" size="50" ><b>Protocols</b></font> | ||
</td> | </td> | ||
| + | <td width="50px"></td> | ||
</html> | </html> | ||
</tr> | </tr> | ||
| Line 46: | Line 47: | ||
**Heat ligation at 65°C for 10 min. | **Heat ligation at 65°C for 10 min. | ||
<br><br><br><br> | <br><br><br><br> | ||
| - | <br></td><td | + | <br></td><td align='center' valign='top' width='300px'> |
| - | <table | + | |
| + | <table border='1'> | ||
| + | <tr bgcolor='yellow'><td>INGREDIENTS: | ||
| + | </td></tr> | ||
<tr><td> | <tr><td> | ||
| - | + | <font class='label'> | |
| - | + | '''Roche T4 Ligase'''<hr color='white' width='70%'> | |
| - | + | '''10X Roche T4 Ligase Buffer'''<hr color='white' width='70%'> | |
| - | </td></tr></table | + | '''ddH2O'''</font> |
| + | </td></tr></table><br><br><br><br> | ||
</td></tr></table> | </td></tr></table> | ||
Latest revision as of 10:33, 21 October 2009
|
Ligation
(estimated time: 20 min + 12-16 hours overnight incubation)
|
|
